具有原核基因启动子活性的家蚕核多角体病毒的DNA片段

本文利用大肠杆菌启动子克隆用质粒pHE5,克隆和分离了家蚕核多角体病毒基因组的8种HindⅢ酶解DNA片段和一种SalⅠ酶解DNA片段。我们发现它们都具有指导抗四环素基因表达的启动子活性。测定了含有这些DNA片段的重组质粒的菌株抗四环素能力。其中最高的可以抗四环素至每毫升约30μg。我们分析了一个DNA片段的部分核苷酸顺序。发现它具有与原核基因启动子极相似的顺序结构。     

DNA FRAGMENTS OF Bombyx mori NUCLEAR POLYHEDROSIS VIRUS CONTAINING THE PROMOTERS ACTIVE IN PROKARYOTES

Using a promoter probe plasmid in E. coli called pHE5, eight different HindⅢ and one SalⅠDNAfragments of Bombyx mori nuclear polyhedrosis virus, directing the expression of the tetracycline re-sistance gene, have been cloned and isolated. The tetracycline resistance levels of the strains containing therecombinant plasmids were measured. Among them, the highest level of the resistance to tetracyclinewas 30μg/ml. Part of the nucleotide sequence of a DNA fragment was determined. A sequence similarto the E. coli promoter was found.