排序方式: 共有4条查询结果,搜索用时 15 毫秒
1
1.
2.
3.
The synthesis of Leu-enkephalin gene by an alternative approach was described in thispaper. The sequence of the synthetic gene, 26 base-pairs in length, was derived from the ami-no acid sequence of the hormone peptide Leu-enkephalin. It bears single-stranded cohesive ter-mini for the restriction endonucleases EcoR I and BamH I so that it may be inserted into apBR 322 plasmid. The 4 deoxyoligonucleotide fragments, varying in length from octanucleotideto octadecanucleotide, were synthesized by an improved phosphotriester method developed inour laboratory. All chemically synthetic fragments were pure and had the correct sequences.The assembly of the Leu-enkephalin gene was carried out in a one-step ligation reaction cata-lysed by T_4-DNA ligase with good yield, Finally, the purified products from polyacrylamidegel electrophoresis had the correct joining-points as predicted. 相似文献
4.
The synthetic leu-enkephalin (LEK) gene was joined with pBR322 and transformed toE. coli. The recombinant plasmids containing the LEK gene were selected by colony hybri-dization, and characterized by restriction mapping and Southern's technique. The lac operonwas used to control the expression of the LEK gene. A recombinant plasmid, pEL 103, inwhich the lac operon and LEK gene are transcribed in the same direction, produces LEK inE. coli. The level of LEK detected by radioimmunoassay reaches 426 ng per mg of bacte-rial protein. 相似文献
1