应用电子显微镜X射线显微分析法研究缺血骨骼肌细胞内钙的分布

在暂时性和持续性缺血组家兔背阔肌切片中,可见不同程度的细胞变性坏死,线粒体中出现中度深染的绒球样结构。在暂时性缺血组还见肌原纤维间有另外两种电子致密颗粒。根据颗粒的分布,大小和结构特征,可认为大颗粒是变性的线粒体,小颗粒是变性的肌质网。X射线显微分析的结果证明,这两种颗粒都含有较高浓度的钙,而绒球样结构仅含微量的钙。这说明光镜下在交性坏死肌细胞中所见的钙沉着,系发生于暂时性缺血肌肉的线粒体和肌质网中。本文结合实验结果对钙沉积的条件作了讨论。     

X-RAY MICROANALYSIS OF CALCIUM IN ISCHEMIC SKELETAL MUSCLE CELLS

Tissue sections of both transiently and persistently ischemic musculus latissimus dorsi of rabbitsshow degenerated and necrotic cells in varying degrees. Degenerated mitochondria appearing in the cellscontain moderate electron-dense fluffy intramatrical deposits. However, only in the transient ischemia sam-ple can other two kinds of electron-dense granules be seen between myofibrils. According to their ultra-structure, size and distribution, the larger granules are considered the degenerated mitochondria, and thesmaller ones the degenerated sarcoplasmic reticulum. X-ray microanalysis proves that these two kinds ofgranules contain high concentration of calcium, while the fluffy deposits contain little calcium. The find-ings suggest that the calcium deposits in the degenerated and necrotic cell seen under light microscopeoccur in mitochondria and sarcoplasmic reticulum of the transiently ischemic muscle cell. The conditionsof calcium accumulation in ischemic muscle cells are discussed.