骨巨细胞瘤组织块的冻存与复温培养观察

本文以10％二甲亚砜或15％甘油为保护剂,在液氮温度(-196℃)下将6例骨巨细胞瘤的组织块保存了3—180天.在复温培养中冻存组织块的各种细胞成分均能存活,其细胞形态、生长行为及乳酸脱氢酶同功酶谱等特点均与用新鲜组织块培养的细胞相同.电子显微镜观察显示二甲亚砜对细胞超微结构的冷冻保护作用优于甘油.本实验证明骨巨细胞瘤组织块的冻存以缓慢降温、快速复温为宜,因为在上述条件下冰晶主要形成于细胞间隙,对细胞损伤较小.     

TISSUE CRYOPRESERVATION AND REWARMING STUDY ON GIANT CELL TUMOR OF BONE

An attempt has been made of cryopreservation of tissue fragments from 6 GCTB surgicalspecimens. They have been kept in 10% DMSO or 15% glycerol medium and stored in liquidnitrogen for periods up to 180 days. Tumor cellular components survive the process of slowfreezing and rapid thawing as cellular growth is successful in subsequent culture. Thoughcertain reversible ultrastructural changes have been detected, no apparent distinction couldbe found in microscopic morphology, motility, growth behavior and LDH isozyme pattern be-tween cultured cells of the cryopreserved fragments and those of the fresh ones. Electronmicroscopy shows that DMSO exceeds glycerol in protecting the ultrastructure of the cells.Slow cooling and rapid thawing is recommended because ice-crystals form in the intercellularspace. which causes only light damage to the cells.