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991.
语言是文化的一个重要部分,没有语言作为工具,人们将无法交流和相互理解;与此相对应,文化也通过语言来体现,各种不同的语言体现着不同的文化。透过中英植物类词语,可看到不同的文化对语言的影响及其在语言中的体现,同时也可以看到不同的语言对其文化的能动作用。 相似文献
992.
Cell cycle regulates proliferative cell capacity under normal or pathologic conditions, and in general it governs all in vivo/in vitro cell growth and proliferation processes. Mathematical simulation by means of reliable and predictive models represents an important tool to interpret experiment results, to facilitate the definition of the optimal operating conditions for in vitro cultivation, or to predict the effect of a specific drug in normal/pathologic mammalian cells. Along these lines, a novel model of cell cycle progression is proposed in this work. Specifically, it is based on a population balance (PB) approach that allows one to quantitatively describe cell cycle progression through the different phases experienced by each cell of the entire population during its own life. The transition between two consecutive cell cycle phases is simulated by taking advantage of the biochemical kinetic model developed by Gérard and Goldbeter (2009) which involves cyclin-dependent kinases (CDKs) whose regulation is achieved through a variety of mechanisms that include association with cyclins and protein inhibitors, phosphorylation–dephosphorylation, and cyclin synthesis or degradation. This biochemical model properly describes the entire cell cycle of mammalian cells by maintaining a sufficient level of detail useful to identify check point for transition and to estimate phase duration required by PB. Specific examples are discussed to illustrate the ability of the proposed model to simulate the effect of drugs for in vitro trials of interest in oncology, regenerative medicine and tissue engineering. 相似文献
993.
Hofer S Ronacher A Horak J Graalfs H Lindner W 《Journal of chromatography. A》2011,1218(49):8925-8936
The aim of this study was to investigate functional increments of ion exchange type ligands, which may improve the performance of mixed-modal ligands for antibody capture out of feed solutions with pH above 6.0 and containing sodium chloride concentrations of 150 mM and higher. For this purpose several functional groups such as sulfonyl, sulfanyl, amide, methoxy, short alkyl and aromatic moieties were tested in combination with a strong sulfonic acid and/or a weak carboxylic acid group. Therefore a series of ligands were synthesized and subsequently coupled onto epoxide activated Fractogel® EMD. In the first instance, all materials were tested by static binding capacity measurements (SBC) under test conditions, comprising a wide variety of different sodium chloride concentrations and differing pH values ranging from 4.5 to 7.5. From these preliminary experiment it was found that especially the aromatic groups improved the binding of human immunoglobulin G (h-IgG) under isotonic conditions, while other increments, e.g. thiophilic or amide groups, were not able to increase the capacity significantly. Taking the SBC results into account, the most promising materials were investigated under dynamic binding conditions (DBC) with a reduced selection of test conditions (pH 5.5, 6.5 and 7.4 at 75 and 150 mM NaCl). N-benzoyl-homocysteine (material J) and 3,5-dimethoxybenzoyl-homocysteine (material K) showed 100% DBCs of 37 mg/mL and 32 mg/mL in the presence of 75 mM NaCl and pH 6.5. Material L carrying mercaptobenzoic acid as a ligand and tested with the same solution provided a 100% DBC of 68 mg/mL. The influence of Pluronic F68 in a mock feed solution as well as in cell culture supernatant was investigated with the best performing bio-affinity type adsorbent, material L. For the real sample feed subsequent SDS-PAGE was conducted for the collected fractions. 相似文献
994.
文化科技融合政策的变迁是文化科技融合创新的发展结果和导向。基于2011—2020年39项政策文本,采用内容分析法和政策文本量化分析等基本统计分析方法的研究表明,文化与科技融合政策的嬗变,是从探索阶段的松散、多点式的初级特征到创新阶段的深层次、宽领域、多方向的系统性布局扩展的,政策内容从简单集中到类型多元,政策目标从相加、相融到深度融合,政策趋向从科技引领、产业主导到双轮驱动、民生为本转变。这些特征客观反映出政府决策关注点、政策结构、政策价值取向的变化,预示着中国文化与科技融合政策将进一步以新发展理念统领文化建设和科技创新,推动制度优势转化效率优势,充分发挥科技创新在文化强国建设中的支撑作用。 相似文献
995.
媒介文化即具有伦理属性的生活方式,被赋予“权力”属性的媒介文化仅系单向度的权能范畴,仅适合于特定的媒介制度。自律失效所致的媒介文化“公地悲剧”可描述为违背法律的、悖离道德的或充满伦理争议的缺陷,技术因素不能证成“公地悲剧”不可避免性。伦理而非权利范畴的媒介文化,其价值倚重在于“内在美”的尺度性、实体而非形式的“公正”品质,内在美排斥了单纯生物性的悦目与感观享受,凸显“理想美”“悲剧美”。蕴涵丰富的实体公正关注超越对善、恶判断的表层认识,更多地寻求在显著符号意义之外所暗含的“外溢效应”。 相似文献
996.
应用组织培养和快速繁殖技术对最适大花蕙兰培养的培养基、激素、培养方式、培养基质等进行了筛选.结果表明,在含有2.0mg/L6-苄氨基腺嘌呤的改良MS培养基中,以液体振荡培养最有利于原球茎的增殖;改良MS培养基 0.3mg/L萘乙酸有利于试管苗的生根;在腐殖土:棉子壳=3:2(体积比)下移栽的成活率最高. 相似文献
997.
张晋峰 《科技情报开发与经济》2008,18(8):87-88
通过问卷调查和实地访谈,对太原市民余日医时间文娱活动与体育活动两方面的现状进行研究,在分析调查结果的基础上提出了一些相关建议。 相似文献
998.
詹雪梅 《科技情报开发与经济》2008,18(15):117-119
论述了作为文化资源大省的河南如何充分利用文化资源的优势发展文化产业的问题,分析了河南文化的历史,提出了发展河南旅游文化的对策与建议。 相似文献
999.
Alexey Sulimov Ivan Ilin Danil Kutov Khidmet Shikhaliev Dmitriy Shcherbakov Oleg Pyankov Nadezhda Stolpovskaya Svetlana Medvedeva Vladimir Sulimov 《Molecules (Basel, Switzerland)》2022,27(17)
Candidates to being inhibitors of the main protease (M) of SARS-CoV-2 were selected from the database of Voronezh State University using molecular modeling. The database contained approximately 19,000 compounds represented by more than 41,000 ligand conformers. These ligands were docked into M using the SOL docking program. For one thousand ligands with best values of the SOL score, the protein–ligand binding enthalpy was calculated by the PM7 quantum-chemical method with the COSMO solvent model. Using the SOL score and the calculated protein–ligand binding enthalpies, eighteen compounds were selected for the experiments. Several of these inhibitors suppressed the replication of the coronavirus in cell culture, and we used the best three among them in the search for chemical analogs. Selection among analogs using the same procedure followed by experiments led to identification of seven inhibitors of the SARS-CoV-2 replication in cell culture with EC values at the micromolar level. The identified inhibitors belong to three chemical classes. The three inhibitors, 4,4-dimethyldithioquinoline derivatives, inhibit SARS-CoV-2 replication in Vero E6 cell culture just as effectively as the best published non-covalent inhibitors, and show low cytotoxicity. These results open up a possibility to develop antiviral drugs against the SARS-CoV-2 coronavirus. 相似文献
1000.
郑淑玉 《黔西南民族师范高等专科学校学报》2020,(2):19-21
自第一部苗族题材电影《山间铃响马帮来》(王为一,1954)问世以来,苗族题材电影共创作出27部影片。苗族题材电影在生动反映苗族文化的同时,也积极参与苗族文化的影像建构。苗族题材电影集中展现了苗族的图腾崇拜、苗族服饰、苗族民居、苗族舞蹈、苗族民俗等苗族非物质文化遗产。 相似文献