ＣaＭ激酶及其在植物体内的功能

介绍了CaM激酶的结构和生化特点，以及植物体内CaM激酶的研究进展。从植物细胞信号转导、发育和基因表达等３个方面讨论了CaM激酶激酶在植物体内的生理功能。     

Development of highly potent inhibitors of the ras-targeting human acyl protein thioesterases based on substrate similarity design

A matter of common sense: A common recognition motif consisting of a negatively charged group five to six bonds away (red) from the (thio)ester functionality (green) and a positively charged tail group ten to twelve bonds away (blue) was identified in two native acyl protein thioesterase?1 (APT1) substrates. This similarity led to the design of potent inhibitors of the Ras-depalmitoylating enzyme APT1.     

重组腺相关病毒基因药物三种滴度的比较与分析

采用酶联免疫吸附测定(ELISA)、实时荧光定量聚合酶链反应(Q-PCR)和转导方法对重组腺相关病毒(rAAV)的衣壳滴度、基因组滴度、转导滴度进行定量,并比较衣壳滴度/基因组滴度(P/GC)和基因组滴度/转导滴度(GC/TU).实验结果表明:3批rAAV的平均衣壳滴度为3.65×10¹³ P·mL^-1,平均基因组滴度为8.67×10¹¹ GC·mL^-1,平均转导滴度为9.85×10⁹ TU·mL^-1,P/GC平均值为41.70,GC/TU平均值为88.20,P/GC和GC/TU平均值接近于AAV2标准品,说明文中方法的制备工艺成熟、稳定,制备的rAAV感染活性较高.     

生物工程技术的开发现状与展望

简述了当代生物工程技术开发和应用的现状，归纳了我国在生物工程领域研究开发所取得的成就，并展望了生物工程技术的未来发展。     

Synthesis and applications of phosphatidylinositols and their analogues

There is an upsurge of interest in the chemistry and biochemistry of phosphoinotides, their analogues and the related enzymes due to their involvement in the inositol phosphates mediated cellular signal transduction pathways. The present review deals with the recent developments in the synthesis and applications of phosphatidylinositol and its derivatives. NCL commun, no. 6014     

Molecular mechanisms of visual excitation: A concatenation of nonlinear cellular processes

The mechanism of transduction in the photoreceptor remains the least understood link in the chain of molecular and cellular processes that are responsible for vision. This paper reviews the molecular species and processes involved in the absorption of light and subsequent biochemical responses of the photoreceptor cell. The cells' electrical response and the probable ionic basis of the response are described. We develop the outlines of a mechanistic model that attempts to link biochemical and electrophysiological responses of photoreceptors of the vertebrate retina.