Determination of Size Distribution of Nano-particles by Capillary Zone Electrophoresis

A new method was developed for the determination of the size distribution of nano-particles by capillary zone electrophoresis (CZE). Scattering effect of nanoparticles was studied. This method for the determination of size distribution was statistical.     

Determination of aristolochic acid by capillary zone electrophoresis

A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.     

Simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by capillary zone electrophoresis

A capillary electrophoretic method for the simultaneous determination of sulfamethoxazole and trimethoprim in plasma was developed. Sulfamethoxazole and trimethoprim extracted from human plasma with ethyl acetate were analyzed at 20 kV and 25 degrees C using 15 mm phosphate buffer (pH 6.2) as the electrolyte. The detection was by UV at 220 nm. The run time was 8.0 min and the limit of quantification was 10.00 microg/mL for sulfamethoxazole and 2.00 microg/mL for trimethoprim. The recovery was >99% for both compounds. This method enabled the detection of sulfamethoxazole and trimethoprim in plasma of patients after oral ingestion of their combined formulation. The present simple and rapid method is applicable to drug monitoring in immunocompromised patients who are taking the combined formulation of these compounds for the treatment or prophylaxis of Pneumocystis carinii pneumonia.     

Determination of oxidized and reduced glutathione, by capillary zone electrophoresis, in Brassica juncea plants treated with copper and cadmium

A rapid method of capillary zone electrophoresis is described to determine the oxidized (GSSG) and reduced (GSH) form of glutathione in plant tissue. In order to separate both analytes in a fused-silica capillary, the pH and composition of the electrolyte solution were optimized. The electrolyte composition was 100 mmol/L, borate 25 mmol/L Tris, and 0.2% w/v metaphosphoric acid (MPA), pH 8.2. Some instrumental conditions used to run the samples were hydrostatic injection for 30 s, 30 kV applied voltage, and UV detection (185 nm) at 25 degrees C. Linearity and useful range obtained for the calibration curves were optimum, with correlation coefficients about 0.999 in the 0-120 micromol/L range. The migration time was highly reproducible, less than 5 min being afforded to run a sample. Electrolyte buffer and samples required a careful pH control for optimal separation of both analytes. This aspect constitutes a critical analytical step when acids are used in the procedure for sample preparation. Simultaneous analysis of GSH and GSSG may provide a useful tool for comparative studies of plants in order to select those species with a potential capacity for detoxification from toxic elements or those appearing promising from phytoremediation for these elements.     

相似路径台风风暴潮过程对比

基于MIKE 21模型和Holland台风风场模型,构建了1909号利奇马和0509号麦莎2次相似路径台风期间的风场和气压场,并模拟了相应的风暴潮过程。结果表明：台风中心附近增水变化显著,浙江东北部海域在台风登陆期间的增水更大;受台风强度影响,台风利奇马期间增水高值区更大,部分海域2次台风期间最大增水差值接近1.0 m;潮致欧拉余流速度较大值主要分布在100 m水深以浅的海域,量值超过20 cm/s,江苏沿海、台湾东北部海域存在欧拉余环流;台风利奇马期间近海斯托克斯余流值普遍小于麦莎期间;斯托克斯余流项对台风期间长江口、浙闽等近海浅水区短期物质输运过程起一定主导作用。用EMD方法和Hillbert变换分析了典型站点风暴潮位的特征,发现站点IMF1模态和天文潮位较吻合;水深和IMF3模态的最大功率谱密度呈现出较高的相关性,并且随着水深的增加,IMF3模态的最大功率谱密度逐步变大。     

Adaptive Constrained Control of Unknown Strict Feedback Nonlinear Systems With Dead Zone Input北大核心CSCD

研究了具有死区输入的预设约束未知高阶严格反馈非线性系统的控制问题,提出了一种基于免疫函数的自抗扰预设漏斗约束自适应控制策略。首先,针对系统内部的未知问题,采用免疫函数与扩张状态观测器结合对系统内部未知项进行观测;其次,通过Lyapunov方法与漏斗控制相结合设计控制器,使得跟踪误差能够维持在预先设定的漏斗约束范围内;同时,利用双曲正切函数速率变化快这一特性设计自适应控制律,引入指令滤波器避免反步法中重复求导问题,分析证明了闭环系统所有信号的有界性。仿真实例表明了控制方法的有效性。     

Evaluation of micellar liquid chromatography and capillary zone electrophoresis for dope control in sport

Micellar liquid chromatography (MLC) and capillary zone electrophoresis (CZE) have been evaluated for the analysis of twelve banned drugs in sport including diuretics and -blockers. In MLC, a sodium dodecylsulphate aqueous solution has been used as mobile phase using an octadecylsilica column. In CZE, a pH 8 buffer solution and a silica capillary have been employed. Parameters of retention and efficiency have been compared. Limits of detection with UV detection at 254 nm and relative standard deviations for atenolol, furosemide, nadolol, spironolactone and triamterene were established and compared in both techniques. Examples of direct urine injection into the separation systems are presented. Drugs overlapping in MLC are well resolved in CZE, while the opposite is true for a limited number of drugs. Some interferences from urine may arise in CZE. The selectivity of analysis would be greatly enhanced by using both techniques, which require only filtration as pre-treatment.     

16MnR钢中疲劳裂纹扩展的细观研究

采用显微疲劳试验方法和显微疲劳试验装置对低合金钢１６ＭｎＲ单边缺口试样进行了疲劳试验,研究了短裂纹萌生和扩展的特征。结果表明,短裂纹萌生及扩展分三个阶段,每一阶段的形貌都有其特点,在裂纹扩展后期,短裂纹的发展以裂纹密度不断增加并形成损伤区为主要特征,然后在损伤区选择一条主裂纹扩展。     

篮球比赛中三分远投运用的探讨

通过现场观察、统计和数据的归纳整理,对远投命中率、个人和战术三分球、远投区域、远投时间和远投队员等进行讨论和分析．篮球比赛中,必须加强终场前的远投战术配合训练,提高战术远投,加强弧顶和两侧底角区域的远投,提高组织后卫和中锋的远投能力,提高远投队员的心理训练水平和对抗能力．     

晋江市潮间带底质,生物体中重金属含量及其分布特征

研究福建省晋江市潮间带底质、生物体内重金属含量的分布特征及其主要影响因素．结果表明：表层底质重金属除总Ｈｇ之外,Ｃｕ、Ｐｂ、Ｃｄ和总Ｃｒ含量在河口、半封闭海湾明显高于紧临台湾海峡的海湾及开敞海区,并且,近年来底质的这些重金属含量大多数呈递增趋势;底质的重金属除总Ｈｇ之外,其他的含量都与底质类型及有机质含量有较好的相关性;３种经济贝类对重金属的富集能力分别为：牡蛎,缢蛏和蛤对Ｃｕ的富集系数分别为ｎ×１０４,ｎ×１０３和ｎ×１０３;３种贝类对Ｐｄ和Ｃｄ的富集系数均为ｎ×１０２．