STUDIES ON PREPARATION OF INACTIVATED VACCINES FROM CELL CULTURES OF MINK ENTERITIS VIRUS (MEV) AND THEIR IMMUNITY

In this paper, superhigh reproductive rate strains of MEV with titre more than HA8192* or TCID50 log9.7 10 have been achieved both by cultivation in cell lines with different susceptibility to MEV and by isolating and identifying in field by the author. The systematic tests proved that S18 and L12 strains of MEV are the best strains for vaccine preparation. In this study, the best means for the tissue cultivation of MEV and the most advanced technological process for the production and detection of serum-free cell-cultured MEV fluids with super-high HA titre in batches in large quantities have been established for the first time. Optimum conditions for MEV inactivation were determined, and safe and effective inactivated vaccines with mineral oil or A1(OH)3 gel adjuvant were successfully prepared with serum-free cell-cultured MEV fluids. Both vaccines with different adjuvants can be manufactured in batches in large quantities and have been widely used all over China since 1986. The change laws of the imm     

Citric acid production from raw glycerol by acetate mutants of Yarrowia lipolytica

Three acetate mutants of the yeast species Yarrowia lipolytica were screened using batch cultivation. The strain Y. lipolytica 1.31 was found to be the most suitable for citric acid production from raw glycerol, a by-product of biodiesel production from rapeseed oil. At the initial concentration of glycerol of 200 g dm⁻³, the citric acid production of 124.5 g dm⁻³, yield of 0.62 g g⁻¹, and productivity of 0.88 g dm⁻³ h⁻¹ were achieved. Presented at the 33rd International Conference of the Slovak Society of Chemical Engineering, Tatranské Matliare, 22–26 May 2006.     

Solubility of 1-Butene in Water and in a Medium for Cultivation of a Bacterial Strain

Solubility measurements of 1-butene in water, from 20 to 50°C and at atmospheric pressure, were carried out using a Ben-Naim/Baer-type apparatus. The experimental results have a precision of about ±0.3%. Using accurate thermodynamic relations, the Ostwald coefficients at the experimental conditions and at infinite dilution, the mole fractions of the dissolved gas at the gas partial pressure of 101.325 kPa and the Henry coefficients at the water vapor pressure were calculated. The mole fraction of dissolved gas were fitted to the Clarke, Glew, and Weiss equation and thermodynamic quantities, standard molar Gibbs energy, entropy, and enthalpy changes, for the process of transferring the 1-butene molecules from the gaseous to the water phase, were computed. Moreover, solubility measurements of 1-butene in an aqueous medium for the cultivation of Xanthobacter Py2 in the same temperature range were also performed at atmospheric pressure. These solubility data are approximately 2.6% lower than those observed in pure water.     

高等学校培养高层次创新人才的积极因素

探讨了高等学校培养创新人才的若干积极因素。在高校推行师生的同化关系，师生之间相互融合，教学相长；对师生的权力进行重新分配，形成互动的互补的课堂氛围；使学生提出质疑成为一种习惯，才能真正地培养出高层次创新性人才。     

浅议对学生创新意识和思维能力的培养

文章试图从解释化学教学中学生创新意识和思维能力的重要性出发，探讨教学过程中如何加强学生创新意识和思维能力的培养，从而鼓励学生创新思维，调动学生学习积极性和主动性。     

用分类与回归树算法进行人才识别

高校人才的引进对学校的发展十分重要．传统的人才识别方式是考察引进人员的一些定性的指标来进行人才识别，这样难以保证识别结果的全面性、科学性和准确性．给出了一种新的定量的人才识别的方法，它是利用数据挖掘中分类与回归树(CART)的方法为理论基础对人才进行定量的识别，更具科学性．分类与回归树方法利用过去已有的引进人才的经验数据分析提取规则，为以后的人才识别提供合理的、科学的决策支持．     

植物学中的探究式教学

文章论述了探究式教学与创新教育的关系；讨论了探究式教学对创新人才培养的意义以及植物学中怎样应用探究式教学。     

培养复合型体育人才是实施《标准》的需要

体育教学是实现学校体育目的任务的基本途径,其实施过程及其效果与多种因素相关,该文通过对《标准》的学习、理解和应用,根据系统原理,从教育理论,教学方法等方面论述了建立一支新型的高素质体育教师队伍的时代要求和现实意义.     

事业单位人才流失现状及对策研究

阐述了人才的重要性，事业单位人才流失现状、原因及防止人才流失的策略。     

药用蜘蛛养殖技术研究

本文介绍了蜘蛛的药用价值与生物学特性,详细分析了蜘蛛的人工养殖及饲养管理技术,还对蜘蛛的疾病防治及蜘蛛的采收与加工做了深入的讨论。