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81.
Clemens?Uanschou Roswitha?Frieht Fritz?PittnerEmail author 《Monatshefte für Chemie / Chemical Monthly》2005,136(8):1365-1381
Summary. In contrast to eukaryotic cells certain eubacterial strains have acquired the ability to utilize L-carnitine (R-(–)-3-hydroxy-4-(trimethylamino)butyrate) as sole source of energy, carbon and nitrogen. The first step of the L-carnitine degradation to glycine betaine is catalysed by L-carnitine dehydrogenase (L-CDH, EC 1.1.1.108) and results in the formation of the dehydrocarnitine. During the oxidation of L-carnitine a simultaneous conversion of the cofactor NAD+ to NADH takes place. This catabolic reaction has always been of keen interest, because it can be exploited for spectroscopic L-carnitine determination in biological fluids – a quantification method, which is developed in our lab – as well as L-carnitine production.Based on a cloned L-CDH sequence an expedition through the currently available prokaryotic genomic sequence space began to mine relevant information about bacterial L-carnitine metabolism hidden in the enormous amount of data stored in public sequence databases. Thus by means of homology-based and context-based protein function prediction is revealed that L-CDH exists in certain eubacterial genomes either as a protein of approximately 35 kDa or as a homologous fusion protein of approximately 54 kDa with an additional putative domain, which is predicted to possess a thioesterase activity. These two variants of the enzyme are found on one hand in the genome sequence of bacterial species, which were previously reported to decompose L-carnitine, and on the other hand in gram-positive bacteria, which were not known to express L-CDH. Furthermore we could not only discover that L-CDH is located in a conserved genetic entity, which genes are very likely involved in this L-carnitine catabolic pathway, but also pinpoint the exact genomic sequence position of several other enzymes, which play an essential role in the bacterial metabolism of L-carnitine precursors. 相似文献
82.
Takashi Hayashita Akiyo Yamauchi Ai-Jun Tong Jong Chan Lee Bradley D. Smith Norio Teramae 《Journal of inclusion phenomena and macrocyclic chemistry》2004,50(1-2):87-94
The design and function of novel supramolecular fluoroionophore/cyclodextrin (CyD) complex sensors for ion and molecule recognition in water are reviewed. For the crown ether fluoroionophore/-CyD complex, the dimerization of the fluoroionophore inside the -CyD is found to be selectively promoted by alkali metal ion binding, thereby resulting in metal-ion-selective pyrene dimer emission in water. This supramolecular function is successfully utilized in the design of a podand fluoroionophore/-CyD complex for sensing toxic lead ion in water. The boronic acid fluoroionophore/-CyD complex binds sugars and produces increased fluorescence emission in water. The response mechanism appears to be due to the suppression of the photoinduced electron transfer (PET) from pyrene donor to trigonal phenylboronic acid acceptor. This is a novel emission function provided by the boronic acid fluoroionophore/-CyD complex sensors in water. 相似文献
83.
A. A. Tyshchenko O. F. Filatova S. M. Khodzhibaeva K. D. Davranov 《Chemistry of Natural Compounds》2004,40(1):75-78
The ability to bind reversibly molecular oxygen was established for a free-radical product of gossypol oxidation. Conversion of dianhydrogossypol into the stable biradical dioxodianhydrogossypol was viewed as the reason for interruption of the gossypol redox conversion cycle in the extracellular milieu of cotton steles. 相似文献
84.
Eupatilin (5,7-dihydroxy-3',4',6-trimethoxy flavone) is an active ingredient of an ethanol extract of Artemisia asiatica (DA-9601) that is used in the treatment of gastritis. In vitro and in vivo metabolism of eupatilin in the rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of eupatilin in the presence of NADPH and UDPGA resulted in the formation of four metabolites (M1-M4). M1, M2, M3 and M4 were tentatively identified as 3'- or 4'-O-demethyl-eupatilin glucuronide, eupatilin glucuronide, 6-O-demethyleupatilin and 3'- or 4'-O-demethyl-eupatilin, respectively. Those metabolites from in vitro study were also characterized in bile, plasma or urine samples after an intravenous administration of eupatilin to rats. In rat bile, plasma and urine samples, eupatilin glucuronide (M2) was a major metabolite, whereas M3, M4 and M4 glucuronide (M1) were the minor metabolites. 相似文献
85.
《Tetrahedron》2004,60(3):529-534
Cyclodextrin glucosyltransferase (CGTase) from Thermoanaerobacter sp. was covalently immobilized on Eupergit C and used for the synthesis of maltooligosyl fructofuranosides employing soluble starch as donor and sucrose as acceptor. Using a weight ratio starch-sucrose of 1:2, the conversion of starch into acceptor products catalyzed by soluble and immobilized CGTases was higher than 80% in 48 h. Under these conditions, the reaction was selective for the formation of maltosyl fructofuranoside. 相似文献
86.
Xi Li Yi Liu Jun Wu Huigang Liang Songsheng Qu 《Journal of Thermal Analysis and Calorimetry》2002,67(3):589-595
The action of three kinds of the selenomorpholine compounds on a strain ofEscherichia coli was studied by microcalorimetry. Differences in their capacities to affect the metabolism of this bacterium were observed.
The extent and duration of the effect on the metabolism as judged from the rate constant (k) of Escherichia coli (in log phase) varied with the different drugs. The kinetics show that selenomorpholine compounds had an effect on the metabolism
process of Escherichia coli. The k of Escherichia coli in the presence of the drugs increased with the increasing concentrations of the drugs (C) at low concentration; but at high concentration, the rate constant decreased with the increasing concentrations of the drugs.
The experimental results reveal that the sequence of antibiotic activity of selenomorpholines is: N-selenomorpholinemethyl
succinimide and its hydrochloride>N-(α-selenomorpholinebenzyl) succinimide.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
87.
《Analytical letters》2012,45(1):171-183
Based on wavelet transformation (WT) and mutual information (MI), a simple and effective procedure is proposed for multivariate calibration of near-infrared spectroscopy. In such a procedure, the original spectra of the training set are first transformed into a set of wavelet representations by wavelet prism transform. Then, the MI value between each wavelet coefficient variable and the dependent variable is calculated, resulting in a MI spectrum; by retaining a subset set of coefficients with higher MI, an update training set consisting of wavelet coefficients is obtained and reconstructed/converted back to the original domain. Based on this, a partial least square (PLS) model can be constructed and optimized. The optimal wavelet and decomposition level are determined by experiment. A NIR quantitative problem involving the determination of total sugar in tobacco is used to demonstrate the overall performance of the proposed procedure, named RPLS, meaning PLS in reconstructed original domain coupled with MI-induced variable selection in wavelet domain (RPLS). Three kinds of procedures, that is, conventional full-spectrum PLS in original domain (FPLS), PLS in original domain coupled with MI-induced variable selection (OPLS), and direct PLS in MI-based wavelet coefficients (WPLS), are used as reference. The result confirms that it can build more accurate and robust calibration models without increasing the complexity. 相似文献
88.
89.
Johannes?Winkler Ernst?Urban Christian R.?NoeEmail author 《Monatshefte für Chemie / Chemical Monthly》2004,135(1):109-116
Summary. A synthesis of the amino sugar 2-amino-2,3-didesoxyribose is described. Starting from D-glucosamine, -methylfuranoside was obtained in eight steps in 20% yield. This carbohydrate is a novel building block for nucleosides and for backbone modified antisense oligonucleotides with 2–5 amide linkages. 相似文献
90.
The cofactors NADH and NADPH, hereafter NAD(P)H [NAD(P)= nicotinamide adenine dinucleotide (phosphate)], belong to the principal endogenous indicators of energetic cellular metabolism. Since the metabolic activity of cells is given by the ratio between the concentrations of free and protein-bound NAD(P)H, the development of autofluorescence techniques which accurately measure the modifications to this ratio is particularly significant. Hitherto the methods applied in the monitoring of cellular metabolism have provided either imprecise results, due to interference of the NAD(P)H signal by perturbing factors, or they have required a complicated internal calibration. We employ biexponential fluorescence lifetime imaging (FLIM) in order to discriminate between the free and protein-bound NAD(P)H without any previous calibration. Thus, we have obtained directly, and for the first time, a high-resolution map of cellular metabolism, that is, an image of the contribution of the protein-bound NAD(P)H to the cumulative NAD(P)H fluorescence signal. Moreover, we demonstrate that protein-NAD(P)H complexes characterised by different fluorescence lifetimes are not uniformly distributed all over the cell, as assumed until now, but are concentrated in certain cellular regions. The different fluorescence lifetimes indicate either different protein-NAD(P)H complexes or different bond strengths between NAD(P)H and the protein in these complexes. Since an important aspect in biological applications is to monitor the dynamics of the relevant processes (such as cellular metabolism), rapid dynamical techniques, for example, rapid biexponential fluorescence lifetime imaging, are needed. Furthermore, it is necessary to reduce the evaluation effort as much as possible. Most of the evaluation techniques in multiexponential FLIM are time-expensive iterative methods. The few exceptions are connected with a loss of information, for example, global analysis; or a loss in accuracy, for example, the rapid evaluation technique (RLD). We implement for the first time in FLIM a noniterative, nonrestrictive method originally developed by Prony for approximations of multiexponential decays. The accuracy of this method is verified in biexponential FLIM experiments in time-domain on mixtures of two chromophores both in homogenous and in heterogeneous media. The resulting fluorescence lifetimes agree (within error margins) with the lifetimes of the pure substances determined in monoexponential FLIM experiments. The rapidity of our evaluation method as compared to iterative pixel-by-pixel methods is evidenced by a reduction of the evaluation time by more than one order of magnitude. Furthermore, the applicability of this method for the biosciences is demonstrated in the investigation of cellular metabolism by means of NAD(P)H endogenous fluorescence. 相似文献