植物中钙解码机制的研究进展

Ca2＋作为重要的第二信使,在植物生长发育中起着非常重要的作用．目前已经鉴定的植物Ca2＋传感器蛋白有：钙调素（CaM）、类钙调素蛋白（CMLs）、钙依赖型蛋白激酶（CDPKs）和类钙调磷酸酶B亚基蛋白（CBLs）及其互作蛋白激酶（CIPKs）,这些传感器蛋白多以基因家族形式存在,并且在植物中能够形成错综复杂的钙离子信号传递网络系统．主要从植物Ca2＋传感器蛋白的生化特性、生理功能和靶蛋白3个方面,综述了植物解码钙信号机制的最新研究进展．     

十堰辣椒根腐病病原菌分离鉴定及高氏15号的生物防治作用研究

为明确辣椒根腐病病原,从田间采集分离、纯化、鉴定、柯赫氏法则验证病原菌,并开展高氏15号生物菌剂对辣椒根腐病的防治效果和促生作用研究.结果表明,通过形态学和分子生物学鉴定(ITS、EF-1a、IGS基因序列比对及进化树分析)发现,引起该地区辣椒根腐病的病原物为茄病镰刀菌(Fusarium solani).柯赫氏法则完成了辣椒根腐病的回接鉴定试验.田间防效和促生结果显示,多粘类芽孢杆菌在240 g/667 m2,高氏15号菌剂在50 g/穴和75 g/穴,甲霜·恶霉灵1 500倍液防治辣椒根腐病效果最好,均达到70%以上.经试验处理的株高、根长、整株鲜重、根鲜重和根干重和空白对照相比有显著差异性.     

三峡工程左岸岸边厂房坝段抗滑稳定研究

三峡工程左岸厂房1~#～5~#机组坝段位于左岸山体缓坡地段,原始地面高程110～140m,坝基开挖高程原定98m,厂房建基面高程最低达22.2m,致使坝下基岩下游边坡开挖形成坡度约54°,临时坡高约70余m的高陡临空边坡.此坝段为缓倾角裂隙相对发育区,建基岩体内有多条倾向下游的长大缓倾角裂隙和少量倾向下游的中倾角裂隙,构成了高陡边坡沿缓(中)倾角结构面的深层滑动稳定问题,对坝基和高陡边坡稳定十分不利.应用不同的计算方法对1~#～5~#机组坝段的抗滑稳定进行计算、校核和验证以确保大坝的稳定安全.     

Natural Products in Preventing Tumor Drug Resistance and Related Signaling Pathways

Drug resistance is still an obstacle in cancer therapy, leading to the failure of tumor treatment. The emergence of tumor drug resistance has always been a main concern of oncologists. Therefore, overcoming tumor drug resistance and looking for new strategies for tumor treatment is a major focus in the field of tumor research. Natural products serve as effective substances against drug resistance because of their diverse chemical structures and pharmacological effects. We reviewed the signaling pathways involved in the development of tumor drug resistance, including Epidermal growth factor receptor (EGFR), Renin-angiotensin system (Ras), Phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt), Wnt, Notch, Transforming growth factor-beta (TGF-β), and their specific signaling pathway inhibitors derived from natural products. This can provide new ideas for the prevention of drug resistance in cancer therapy.     

机顶盒硬件接口信号的研究及应用

数字电视机顶盒的技术含量非常高,它集中反映了多媒体、计算机、通信技术和网络技术的发展水平.本文对其接口信号进行的分析比较研究,对于数字电视信号的高质量传输,硬件设备的合理配置以及充分发挥音视频设备的最大效能,具有重要的实践意义.     

结构化主体信息披露过程中相关问题研究

《企业会计准则第41号——在其他主体中权益的披露》的实施,使结构化主体备受关注.在国际会计准则理事会发布《国际财务报告准则第12号——在其他主体中权益的披露》的背景下,我国的第41号准则充分表达了国际趋同.针对第41号准则的核心内容——结构化主体做出分析,对信息披露存在的问题进行探讨,指出存在的问题并提出了改进的相关建议.     

Screening and identification of Caulis Sinomenii bioactive ingredients with dual‐target NF‐κB inhibition and β2‐AR agonizing activities

Caulis Sinomenii (CS) is a valuable traditional medicine in China. Its extract can act as an anti‐inflammatory agent and a vascular smooth muscle relaxant. However, the underlying mechanisms remain unknown. In this study, we developed a simple dual‐target method based on ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry combined with a dual‐target bioactive screening assay for anti‐inflammatory and antispasmodic activities to characterize the chemical structure of various bioactive compounds of CS rapidly. Seven potential NF‐κB inhibitors were identified, including laudanosoline‐1‐O‐xylopyranose, 6‐O‐methyl‐laudanosoline‐1‐O‐glucopyranoside, menisperine, sinomenine, laurifoline, magnoflorine and norsinoacutin. Furthermore, IL‐6 and IL‐8 assays confirmed the anti‐inflammatory effects of these potential NF‐κB inhibitors, in which laudanosoline‐1‐O‐d ‐xylopyranose and menisperine were revealed as novel NF‐κB inhibitors. Among the seven identified alkaloids, three potential β₂‐adrenergic receptor agonists, including sinomenine, magnoflorine and laurifoline, were characterized using a luciferase reporter system to measure for the activity of β₂‐adrenergic receptor agonists. Finally, sinomenine, magnoflorine and laurifoline were identified not only as potential NF‐κB inhibitors but also as potential β₂‐adrenegic receptor agonists, which is the first time this has been reported. Molecular dynamic simulation and docking results suggest that the three dual‐bioactive constituents could not only inhibit Pseudomonas aeruginosa PAK strain‐induced inflammatory responses via a negative regulation of the Braf protein that participates in MAPK signaling pathway but also activate the β₂‐adrenegic receptor. These results suggest that CS extract has dual signaling activities with potential clinical application as a novel drug for asthma.