Characterization of a putative Xylella fastidiosa diffusible signal factor by HRGC-EI-MS

Xylella fastidiosa (X.f.) is a plant pathogen with high levels of genomic similarity to Xanthomonas campestris pv. campestris (X.c.c.). It has been shown that X. fastidiosa synthesizes a putative diffusible signal factor (X.f.-DSF) that activates regulation of pathogenicity factor (rpf) genes in a X.c.c. reporter system, which might be involved in the regulation of pathogenesis associated genes as in X.c.c., as well as in quorum-sensing. The nature of the X.f.-DSF is not known, whereas the X.c.c.-DSF has been identified as cis-11-methyl-2-dodecenoic acid. In this work, the chemical nature of a putative X.f.-DSF molecule, able to restore endoglucanase activity in a X.c.c. rpfF mutant, was investigated as if it was a fatty acid derivative. Bioassays with X.c.c. reporter bacterium and X.f. culture extracts, based on endoglucanase restoration activity, were also carried out in order to confirm the DSFs molecules similarities. For this reason, a gas chromatography-mass spectrometry method was developed with standard fatty acids methyl esters mixtures. The retention time, as well as the fragmentation patterns, of each standard was used to identify the DSF molecule synthesized by X.f. in the culture medium. Typical ester fragmentation patterns (the derivatized analyte) were observed, such as: McLafferty rearrangement and migration of the Hdelta followed by 1,4-hydrogen shift and cleavage of the bond Cbeta-Cgamma, confirming the nature of this molecule. This confirmation was corroborated by the common peaks in both spectra. Besides, the observed retention time reinforces our conclusion since it corresponds to a methyl ester with 15 carbons. Since the X.f.-DSF molecule was tentatively identified as 12-methyl-tetradecanoic acid (by mass spectra library comparison), this standard compound was also analyzed, strongly suggesting that this is the identification of such a molecule. To our knowledge, this is the first time a DSF produced by X.f. has been characterized.     

碘喷孔位置的变化对激光性能的影响

 在主、副气流流量不变的情况下,仅改变氧碘化学激光器中碘喷孔的位置,数值模拟了其混合气体流场特性和小信号增益分布等的变化。计算结果表明,碘喷孔在喷管叶片上的位置沿气流方向往下游移动,混合状况变差,光腔中小信号增益降低,输出功率减小,饱和光强沿气流方向的衰减变得平缓。     

红外探测器光电性能测量系统方案研究

本文提出了一种新型的由微机控制的全自动红外探测器件光电性能测量系统,针对系统的硬件组成部分及其设计思想做了详细的讨论.     

Time-Domain Quantification of Multiple-Quantum-Filtered Na Signal Using Continuous Wavelet Transform Analysis

The application of continuous wavelet transform (CWT) analysis technique is presented to analyze multiple-quantum-filtered (MQF) ²³Na magnetic resonance spectroscopy (MRS) data. CWT acts on the free-induction-decay (FID) signal as a time-frequency variable filter. The signal-to-noise ratio (SNR) and frequency resolution of the output filter are locally increased. As a result, MQF equilibrium longitudinal magnetization and the apparent fast and slow transverse relaxation times are accurately estimated. A developed iterative algorithm based on frequency signal detection and components extraction, already proposed, was used to estimate the values of the signal parameters by analyzing simulated time-domain MQF signals and data from an agarose gel. The results obtained were compared to those obtained by measurement of signal height in frequency domain as a function of MQF preparation time and those obtained by a simple time-domain curve fitting. The comparison indicates that the CWT approach provides better results than the other tested methods that are generally used for MQF ²³Na MRS data analysis, especially when the SNR is low. The mean error on the estimated values of the amplitude signal and the apparent fast and slow transverse relaxation times for the simulated data were 2.19, 6.63, and 16.17% for CWT, signal height in frequency domain, and time-domain curve fitting methods, respectively. Another major advantage of the proposed technique is that it allows quantification of MQF ²³Na signal from a single FID and, thus, reduces the experiment time dramatically.     

最大熵谱的振幅谱和相位谱计算

最大熵谱估计是高分辩率的现代谱估计方法,它有着传统谱估计方法不可比拟的优越性。但最大熵谱估计中振幅失真,使它的应用受到很大限制。本文着重介绍了运用准确最小二乘法计算最大熵谱信号的最佳频率成分,再利用三角函数回归法求取原始信号的真实振幅谱和相位谱。对最佳频率成分位置的确定,采用-种既简便又准确的方法,比前人的方法更为优越。     

一种X射线聚焦光学及其在X射线通信中的应用

基于栅控脉冲发射X射线源与单光子探测技术的X射线通信已经实现了实验室语音通信验证,并对通信系统的误码率性能进行了分析,为探索未来X射线深空应用打下了坚实的基础.针对目前X射线通信面临的信号发散角大、通信距离短、难以实现工程化应用的情况,迫切需要对X射线通信天线系统进行深入研究.为了提高信号增益、增大X射线通信的距离,提出了多层嵌套式X射线聚焦光学作为X射线通信的"收发天线",理论分析了X射线聚焦光学用于X射线通信"收发天线"的可行性,分析了X射线聚焦光学的理论基础与结构设计,对"发射天线"发散角、"接收天线"有效面积与焦斑尺寸、信号增益等性能做了探讨.结果表明:在信号发射端,"天线"的发散角为3 mrad左右,发射增益23 d B;在信号接收端,"接收天线"的有效面积5700 mm2@1.5 keV,焦斑直径为4.5 mm,接收增益为25 d B,通信系统总的增益可达48 d B.     

Wavelet analysis in testing signals

I.IntroductionAseriesofnon-stationary,shocksignalsareoftenpresentedinengilleeringpractice.Forexample,thevibrationresponseofautomobilesrunningonanunevenroad,aircraftstlyinginchangingairflowandtheresponseofhighbuildingstructurestoearthquakewaves.Thetraditionaltime-frequencyanalysingmethodwhichisbasedonFouriertranstlormcanonlyprovidetheaveragestatisticalresultsinanalysingshocksignals.It'sdiftlcultl'OrFourierTransformtodiscribetilelocalandprecisecharacteristicsofnon-stationarychocksignals.ButW…     

HP数据采集系统与IBM计算机的实验数据通讯

HP计算机数据采集系统的先进性和工作可靠性勘称当今世界领先,但由于它与IBM机的不兼容性使它的应用受一定的限制。本文介绍的通讯方法成功地解决了这一问题,使得HP计算机数据采集系统所获得的实验数据可方便地传送给IBM计算机进行数据处理或存盘保留,从而扩大了数据采集系统所获得数据的应用范围。     

Singularly perturbed nonlinear second order elliptic equation

In this paper, we study singular perturbation problems of some semi-linear second order elliptic equations with nonlinear boundary value conditions: where ε is a small positive parameter and u/ l is a directional derivative, which lies on an oblique vector (x,ε). We have given a construction of the asymptotic solutions and proof of their asymptotic correctness, which is based on the principle of contraction mapping.     

行波管三维非线性计算机模拟的改进

采用宏粒子模型计算空间电荷力,导出行波管三维大讯号工作方程组,开发出ＶｉｓｕａｌＣ＋＋数值分析软件。随后进行计算机模拟,提出将三维空间电荷力处理为二维,大大节省了计算时间,数值结果与三维计算吻合很好。