Microfluidic‐Printed Microcarrier for In Vitro Expansion of Adherent Stem Cells in 3D Culture Platform

Microcarrier‐based stem cell expansion cultures can increase the dimensions of in vitro stem cell cultures from 2D to 3D. The culture handling process then becomes more efficient compared with conventional 2D cultures. However, the use of spherical plastic microcarriers complicates the monitoring of cell culture. To facilitate monitoring, transparent disc‐shaped microcarriers are manufactured using a light‐initiated microfluidic printing system and the obtained microcarriers are named as 2.5D microcarrier. The 2.5D microcarriers (diameter/height ≈ 5) enable us to use conventional monitoring tools in 2D‐based platform during the in vitro expansion on a 3D culture platform. Surface modification via a 1 h‐long poly‐dopamine (PDA) reaction can maintain the transparent nature of the microcarriers while optimizing the cell attachment. The surface marker expression and differentiation potential of the 2.5D microcarrier‐expanded stem cells reveal that the characteristics and functionalities preserved during expansion. The 2.5D microcarrier is readily integrated into an on‐bead assay to conserve reagents and permit a high number (n = 9) of repeated measurements with reliable results. These results demonstrate that the 2.5D microcarrier‐based scale‐up culture provides a valuable tool for the in vitro expansion of adherent stem cells, especially if repetitive monitoring is required.     

TTF-TCNQ complex based printed biosensor for long-term operation

A printed amperometric glucose sensor based on glucose oxidase adsorbed on crystals of tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) is described. The sensitivity and the stability of the sensor are affected by the binder and solvent used for the preparation of the GOD.TTF-TCNQ paste. The sensors are continuously used in a flow injection analysis (FIA) system under continuous polarization at 0.15 V (vs Ag/AgCl) at 37°C. The developed sensors exhibit a large response current, an extended linear range and oxygen independence. The sensors can be used for more than 3 months. The GOD.TTF-TCNQ paste is suitable for the preparation of planar sensor by screen printing method.     

On the origin of printing in the light of new archaeological discoveries

30 years have pas. since a printeddhārani sūtra scroll was discovered in 1966 in Kyongju. Korea. However. there have been different views about the date and place of its printing and publication in Korea and abroad. Some Korean scholars think that it was translated into Chinese from Sanskrit in 704 in China and printed during 706–751 in the Silla period in Korea. After a further research it is now proved that this sutra was translated into Chinese in 701 and printed in the following year, 702, in Luoyang, during the reign of Empress Wu. This shows that the sutra could not have been printed in the Silla period. The discoveries of theSnddharma pundarik sūtra printed during 690–699 and the Sanskritdharani charm printed around 650–670 in China also show that thedhārani sutra found in Korea is not the earliest one. Both literary records and archaeological findings prove that printing originated in China.     

Synthesis of patterned polymer brushes from flexible polymeric films

Surface-initiated polymerizations were carried out from polymeric surfaces of commercially important polyester films, poly(ethylene terephthalate) (PET) and poly(ethylene naphthalate) (PEN). These plastic films were modified prior to polymerization by plasma oxidation, exposing surface hydroxyl groups, in order to immobilise patterned self assembled monolayers (SAMs) of trichlorosilane initiator, through the soft lithographic method of microcontact printing (μCP). Subsequently, polymerizations were initiated from the surface via controlled atom transfer radical polymerization (ATRP), under aqueous conditions, to create patterned brushes of the thermo-responsive polymer poly(N-isopropyl acrylamide) (PNIPAM). By creating patterned, rather than homogeneous brushes characterization was made possible by atomic force microscopy (AFM).     

基于溶胶-凝胶及印制技术制备复合陶瓷碳pH电极

将溶胶－凝胶技术与丝网印制技术相结合研制了一种新型复合陶瓷碳pH电极和参比电极，并组合制备了PH复合电极，PH电极利用天然沸石作质子接受体，用溶胶－凝胶法进行固定，对溶胶－产法与印制技术相结合制作包埋沸石的复合陶瓷碳敏感的条件进行了实验，并对电极参数进行了测试，该电极测定范围为PH1－12，响应斜率为0.06V/dec。电极稳定性，重现性好，响应愉，利用此电极对多种水样进行了分析，取得了满意效果，采用该法制备离子选择性电极具有简易，价廉，易批量化生产和推广应用等特点。     

Fourier transform infrared spectroscopy applied to ink characterization of one-penny postage stamps printed 1841-1880

Fourier transform infrared spectroscopy (FTIR) was applied to the characterization of red inks used to print one-penny stamps in Britain during the period 1841-1880.Micro- and macro-accessories were compared in both transmission and reflection mode. The best spectra were obtained using a microscope with a diamond cell coupled to an infrared spectrometer. In this case, the extraction of a single paper fibre was enough to obtain a good spectrum. Calcium carbonate, calcium sulfate, lead chromate, cyano compounds, cellulose, and oil were identified. When bigger surfaces need to be analyzed, diffuse reflection or variable specular reflectance accessories can be used. Nevertheless, only cyano compounds and calcium carbonate can be characterized.Results are in agreement with those obtained using scanning electron microscopy.The chronological order of the 71 stamps studied showed that the chemical composition of the red ink changed during the period studied.     

胶印制版工艺数据化与规范化研究

对胶印制版工艺的数据化和规范化进行了详细的理论分析，设计了印刷测试版，并应用最新的G7控制工艺，在陕西省印刷厂进行了打样工艺的数据化与规范化研究。实验获得了网点扩大量、网点补偿量以及灰平衡数据，提高了印刷品质量及质量稳定性，为胶印制版工艺数据化与规范化研究提供了参考。     

丝网印刷制版重氮感光胶及其光化学反应机理

分析了丝网印刷制版重氮感光胶的组成、性质、用途、基本配方、配制及其光化学反应机理，有利于丝网印刷工作者根据需要配制出个性化的感光胶。     

论服饰印花在服装设计中的作用

从服饰印花所具有的特性和印花方法、工艺多样性方面展开分析,论述了服饰印花在服装设计中的重要作用．     

内电解-混凝-生化工艺处理长毛绒染色废水的试验研究

进行了用内电解－混凝土－生化工艺对长毛染色废水处理试验研究,结果表明：该工艺对可生化性很低的废水ＣＯＤ和色度具有较高的去除率,而且投资省,运行费用低、操作简便、在原水ＣＯＤＣＲ为４００－７００ｍｇ／Ｌ时,出水ＣＯＤＣＲ低于１００ｍｇ／Ｌ;原水色度为２００－３００倍时,出水色度低于５０保。