QTL mapping of resistance to sheath blight in maize（Zea mays L.）

Maize sheath blight (Rhizoctonia Solani) is a widely occurring fungus disease with great harm to corn-pro- ducing regions in the world. The first happening of sheath blight in China was reported in Jilin Province as early as in 1966[1]. Since the 1970s, the enlargement of corn- growing regions, the application of maize hybrids, the increasing use of fertilizers, especially the nitrogenous fertilizer, and a higher growth-density, all have caused a quick spread of sheath blight, the occurring …     

Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE‐ESI‐MS/MS system

We report the first use of CZE for absolute characterization of host cell proteins (HCPs) in recombinant human monoclonal antibodies. An electrokinetically pumped nanoelectrospray interface was used to couple CZE with a tandem mass spectrometer. Three isotopic‐labeled peptides (LSFDKDAMVAR, VDIVENQAMDTR, and LVSDEMVVELIEK) were synthesized by direct incorporation of an isotope‐labeled lysine or arginine. The heavy‐labeled peptides were spiked in the HCP digests at known concentrations. After CZE‐ESI‐MS/MS analysis, the peaks of native and isotopic‐labeled peptides were extracted with mass tolerance ≤ 5 ppm from the electropherograms, and the ratios of peak area between native and isotopic‐labeled peptides pairs were calculated. Calibration curves (the ratios of peak area versus spiked peptide amount) with R² values of 0.999, 0.997, and 0.999 were obtained for the three HCP peptides, and the absolute amounts of the three proteins present were determined to be at the picomole level in a 20 μg sample of digested HCPs. The target proteins were present at the 7–30 ppt level in the purified HCP samples.     

Numerical study of the effect of secondary electron emission on the sheath characteristics in q-non-extensive plasma

In this paper, a plasma sheath containing primary electrons, cold positive ions, and secondary electrons is studied using a one-dimensional fluid model in which the primary electrons are described by q-non-extensive distribution according to the Tsallis statistics. Based on the Sagdeev potential method and the current balance relation, a modified sheath criterion, and floating potential are established theoretically. The effect of secondary electron emission on q-non-extensive plasma sheath characteristics have been numerically examined. A significant change is observed in the quantities characterizing the non-extensive plasma sheath with the presence of the secondary electrons. It is found that the sheath properties with super-extensive distribution and sub-extensive distribution are different compared with plasma sheath with Maxwell distribution .     

激波风洞设施中的等离子体包覆目标电磁散射实验研究

利用JF10高焓激波风洞设施, 进行了等离子鞘包覆目标的电磁散射测量实验. 基于矢量网络仪的步进扫频体制, 在C波段进行实验, 观测到等离子鞘对目标雷达散射截面(radar cross section, RCS)的影响. 并且, 目标散射测量值中直接体现了激波风洞的高速气流状态信息: 气流前段会造成散射回波的剧烈变化且稳定性差, 持续0.5–1 ms; 激发的等离子鞘有效持续时间仅约为2 ms, 衰减了目标RCS回波.     

水泥环弹性模量对套管外挤载荷的影响分析

利用弹性力学解析及有限元方法,分别研究了均匀地应力和非均匀地应力条件下水泥环弹性模量对套管外挤载荷的影响规律。研究结果表明,增加水泥环弹性模量对套管外挤载荷有一定的减缓作用,但效果远没有降低水泥环弹性模量明显。理想水泥环的性能应该是高强度、低刚度。在固井作业时,对软地层推荐采用弹性模量大的水泥,对硬地层推荐采用弹性模量小的水泥。     

水稻同工酶与叶鞘颜色连锁遗传的研究

通过水稻两个杂交组合三个性状的遗传特点和相互关系的研究，表明水稻酯酶同工酶１２Ａ，过氧化物酶１５Ａ和叶鞘颜色分别受三个基因位点（ＥＳｔ—３，Ｐｏｘ—４和Ｐｓｈ）控制。三个性状间存在明显的连锁关系。三点测交结果表明，控制上述三个性状的基因间的排列顺序是Ｐｓｈ～Ｅｓｔ—３～Ｐｏｘ—４。基因间的遗传距离为，Ｐｓｈ～Ｅｓｔ—３之间５．６，Ｅｓｔ—３～Ｐｏｘ—４之间１３．５．     

丁丙诺啡对小鼠中脑导水管周围灰质Ca~(2)分布的影响:电镜及电子探针研究

用透射电镜及电子探针X-射线显微分析法研究了丁丙诺啡(Buprenorphine,BN)引起镇痛期间小鼠中脑导水管周围灰质区钙离子分布的改变.按照改进的Komnick方法,脑组织用含有2％焦锑酸钾的1％锇酸固定.实验结果表明,动物经腹腔注射BN(0.8mg/kg)30分钟后,在髓鞘、轴突、线粒体和细胞核中均可见到电子致密的沉淀颗粒,尤其在髓鞘的环状片层中形成大量的、密集的颗粒状沉淀.电子探针X-射线显微分析证实髓鞘中的沉淀颗粒含有元素钙,提示BN镇痛时髓鞘结合大量的钙离子,并且可能经过髓鞘的转运,钙离子进入轴突,贮存于线粒体中.本文讨论了在中枢神经系统中的钙离子转运的可能途径.     

探针电流调制法在射频低压等离子体诊断中的应用

应用探针电流调制法设计的三探针用于诊断无内电极放电的射频低压等离子体中的电子能量分布函数。测量了氮气等离子体在压强为０．１３～１３Ｐａ时的电子能量分布函数。由此得到的电子平均能量，与由探针Ｖ－Ｉ特性计算得到的电子平均能量相差不超过５％，证明了本测量系统的可靠性。     

气体放电阴极鞘层氩离子的自洽蒙特卡罗模拟

建立了气体放电阴极鞘层离子的自洽蒙特卡罗模拟模型,考虑了离子与中性原子的电荷交换碰撞和弹性散射,用到了精确依赖于离子能量的电荷交换和动量输运截面。模拟了氩离子在阴极鞘层中的运动,得到了不同气压下自治电场分布,离子的能量分布和角分布,发现:离子由鞘层边界向阴极运动过程中,离子能量分布的高能部分逐渐增大,角分布向小角度部分压缩,鞘层中的强电场加速和聚焦了离子;在鞘层边界附近的电场呈非线性。     

重粒子在阴极鞘层中输运的理论模型

建立了重粒子在阴极鞘层中输运的理论模型,推导出离子、中性粒子入射阴极表面速度分布 函数和到达阴极的离子流、中性粒子流的解析表达式.理论结果与蒙特-卡罗模拟相一致. 关键词： 阴极鞘层 重粒子输运 理论模型