硼上去烃化反应中的共生效应

考察三（２－苯并呋喃基）硼烷、三（２－呋喃基）硼烷及三（２－噻吩基）硼烷被某些β－二酮（乙酰丙酮、二苯甲酰基甲烷、二茂铁基甲酰丙酮）去烃化的能力，及其产物β－二酮螯合物在电子电离质谱反应中的再去烃化差异．结果证实，受共生效应支配，共生性配体２－苯并呋喃核及２－呋喃核比非共生性配体２－噻吩核均较难从硼原子上裂去．     

改进的变电站蓄电池内阻测量方法的研究与系统设计

蓄电池作为变电站直流系统的备用电源,亦为关键电源失掉后的最后防线,其可靠性日益受到人们的重视.很多研究人员早已致力于蓄电池智能安全监测系统的研究,研究表明,蓄电池的内阻与荷电程度有较高的相关性,通过测量蓄电池内阻可较准确地预测其剩余容量.目前,国内外普遍认可四线法测量蓄电池内阻,但是这种方法的数据处理相当复杂.在该方法测量原理的基础上,提出了一种新的测量数据处理方法,很大程度上简化了测量.并详细介绍了该方法的原理和应用系统设计实现过程.实验表明,该方法简单可靠,精度高,具有良好的实用价值和推广意义.     

基于Proteus的电子电路设计研究

本文使用Proteus对典型的函数信号发生器（模拟电路）、四路彩灯（数字电路）和数显直流稳压电源（模数混合电路）进行了设计与分析,仿真结果表明,在电子工程设计领域,利用Proteus进行电子电路设计,既省时省力省材、低碳环保,又可以降低设计成本、提高设计效率．     

戴维南定理验证实验错误结果的分析

指出了戴维南定理验证实验存在理论分析和实测结果不相同的问题，分析了问题产生的原因，提出了解决问题的方法。     

A Diffusion Approximation for a GI/GI/1 Queue with Balking or Reneging

Consider a single-server queue with a renewal arrival process and generally distributed processing times in which each customer independently reneges if service has not begun within a generally distributed amount of time. We establish that both the workload and queue-length processes in this system can be approximated by a regulated Ornstein-Uhlenbeck (ROU) process when the arrival rate is close to the processing rate and reneging times are large. We further show that a ROU process also approximates the queue-length process, under the same parameter assumptions, in a balking model. Our balking model assumes the queue-length is observable to arriving customers, and that each customer balks if his or her conditional expected waiting time is too large.     

MOS管沟道长度及衬底调制效应对其特性曲线影响的计算机模拟

借助CAD电路仿真工具PSpice模拟研究了MOS管沟道长度调制效应及衬底调制效应对其特性曲线的影响。     

电热原子化器的单片机程控电源研制

用高性能、低功耗的微处理器与直流稳压电源结合,设计了用于电热原子化器实验装置的程控电源。同时,制作了原子化器电源的供给电路和信号控制电路,还编写了硬件的控制及信号处理软件。用该程控电源结合钨丝电热原子化器对实际样品进行了测定,其结果令人满意。由于采用了单片机,使得电源简单、小巧、价廉,能满足电热原子光谱法实验过程中的实际需求。该程控电源具有方便、可靠、性价比高的特点。     

An analytical approach for on-site analysis of breath samples for Δ9-tetrahydrocannabinol (THC)

Increased acceptance of cannabis containing the psychoactive component, Δ9-tetrahydrocannabinol (THC), raises concerns about the potential for impaired drivers and increased highway accidents. In contrast to the “breathalyzer” test, which is generally accepted for determining the alcohol level in a driver, there is no currently accepted roadside test for THC in a motorist. There is a need for an easily collectible biological sample from a potentially impaired driver coupled with an accurate on-site test to measure the presence and quantity of THC in a driver. A novel breath collection device is described, which includes three separate sample collectors for collecting identical A, B, and C breath samples from a subject. A simple one-step ethanol extraction of the “A” breath collector sample can be analyzed by UHPLC/selected ion monitoring (SIM) liquid chromatography/mass spectrometry (LC/MS) to provide qualitative and quantitative determination of THC in breath sample in less than 4 min for samples collected up to 6 h after smoking a cannabis cigarette. SIM LC/MS bioanalyses employed d3-THC as the stable isotope internal standard fortified in negative control breath samples for quantitation including replicates of six calibrator standards and three quality control (QC) samples. Subsequent confirmation of the same breath sample in the B collectors was then confirmed by a reference lab by LC/MS/MS analysis. Fit-for-purpose bioanalytical validation consistent with pharmaceutical regulated bioanalyses produced pharmacokinetic (PK) curves for the two volunteer cannabis smokers. These results produced PK curves, which showed a rapid increase of THC in the breath of the subjects in the first hour followed by reduced THC levels in the later time points. A simpler single-point calibration curve procedure with calibrators and QC prepared in ethanol provided similar results. Limitations to this approach include the higher cost and operator skill sets for the instrumentation employed and the inability to actually determine driver impairment.     

Molecular Dynamics Simulations in Designing DARPins as Phosphorylation-Specific Protein Binders of ERK2

Extracellular signal-regulated kinases 1 and 2 (ERK1/2) play key roles in promoting cell survival and proliferation through the phosphorylation of various substrates. Remarkable antitumour activity is found in many inhibitors that act upstream of the ERK pathway. However, drug-resistant tumour cells invariably emerge after their use due to the reactivation of ERK1/2 signalling. ERK1/2 inhibitors have shown clinical efficacy as a therapeutic strategy for the treatment of tumours with mitogen-activated protein kinase (MAPK) upstream target mutations. These inhibitors may be used as a possible strategy to overcome acquired resistance to MAPK inhibitors. Here, we report a class of repeat proteins—designed ankyrin repeat protein (DARPin) macromolecules targeting ERK2 as inhibitors. The structural basis of ERK2–DARPin interactions based on molecular dynamics (MD) simulations was studied. The information was then used to predict stabilizing mutations employing a web-based algorithm, MAESTRO. To evaluate whether these design strategies were successfully deployed, we performed all-atom, explicit-solvent molecular dynamics (MD) simulations. Two mutations, Ala → Asp and Ser → Leu, were found to perform better than the original sequence (DARPin E40) based on the associated energy and key residues involved in protein-protein interaction. MD simulations and analysis of the data obtained on these mutations supported our predictions.     

柴油机燃用F-T/生物柴油混合燃料的排放性能研究

以未做调整的四缸增压中冷发动机为试验样机,分别燃用不同比例的F-T/生物柴油混合燃料,对混合燃料的常规排放尤其是非常规排放进行了对比研究。结果表明:F-T/生物柴油混合燃料相对于0#柴油可明显改善NO_X、CO和碳烟的排放,但随着生物柴油比例的提高,NO_X、CO排放略有升高,碳烟排放则随着生物柴油比例增加而降低;外特性下,混合燃料随着生物柴油比例的提高,HC排放相对于0#柴油有明显下降;非常规排放中混合燃料可有效降低甲醛排放;SO_2排放也有明显下降,但随生物柴油配比的增加而有上升的趋势。混合燃料能有效抑制苯类排放。