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101.
The piezoelectric bulk acoustic wave (BAW) impedance analysis method was employed to monitor in situ the proteinase-catalyzed hydrolyzation of casein and the effect of pesticide D-allethrin on the proteinase activity in the midgut of the spider Pardosa pseudoannulata, and the results obtained were verified by ultraviolet spectroscopy. The hydrolyzation was significantly increased in the presence of low-dose D-allethrin, whereas significantly inhibited in the presence of high-dose D-allethrin, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of D-allethrin, with a significant frequency response observed even at a 1.5×10-5 volume ratio of D-allethrin to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose D-allethrin pesticide and quick detection of proteinase activity. 相似文献
102.
The molecular evolutionary tree, also known as a phylogenetic tree, of the serine proteinase superfamily was constructed by means of structural alignment. Three-dimensional structures of proteins were aligned by the SSAP program of Orengo and Taylor to obtain evolutionary distances. The resulting evolutionary tree provides a topology graph that can reflect the evolution of structure and function of homology proteinase. Moreover, study on evolution of the serine proteinase superfamily can lead to better understanding of the relationship and evolutionary difference among proteins of the superfamily, and is of significance to protein engineering, molecular design and protein structure prediction. Structure alignment is one of the useful methods of research on molecular evolution of protein. 相似文献
103.
The interaction betweenBacillis subtilis neutral proteinase (B.S.NP) and inorganic metal compounds (CoCl2, NiCl2) was investigated by1H NMR spectroscopy. It has been shown that the Zn(II) ion in the active center of the native enzyme may directly interact with external CoCl2 and NiCl2, producing Co(II)- and Ni(II)-substituted derivatives, and their1H NMR spectra were obtained for the first time. From the1H NMR spectra, the coordinated structure of the active center in the native enzyme was described. 相似文献
104.
沈萍;王小英 《武汉大学学报(理学版)》1991,(1):109-113
用嗜麦假单胞菌（Pseudomonas maltophilia）的静息细胞悬液研究了高温对该菌产生胞外蛋白酶的影响。细胞在37℃培养30min 后,其产酶能力完全受到抑制。将在30℃合成蛋白酶的细胞置37℃处理30min 后重新放回30℃时,有一个30min 的延缓期。利福平能阻止细胞在30℃恢复蛋白酶的合成,当细胞经37℃处理30min 后,利福平非敏感的蛋白酶合成能继续30min。实验结果表明;细胞经37℃处理30min 后,合成蛋白酶的 mRNA 库遭到破坏,延缓期反映了新的 mRNA 库的合成。 相似文献
105.
106.
嗜热蛋白酶生产菌的筛选 总被引：4，自引：0，他引：4
张云峰 《西南师范大学学报(自然科学版)》2006,31(2):157-161
从温泉水、高温堆肥等样品中初筛到62株嗜热蛋白酶生产菌,进一步复筛出一株高产嗜热蛋白酶的生产菌株DPE7(菌株来自温泉水),该菌株被初步鉴定为芽孢杆菌(Bacillus sp．)．嗜热蛋白酶生产的最适碳源是葡萄糖, 最佳氮源为酵母粉加硫酸铵,Ca2 对菌株产酶具有促进作用．在65℃、175 r／min、pH 9的产酶培养基中振荡培养 24 h后,发酵液中的酶产量高达23．4 U／mL(60℃测定)． 相似文献