负载扰动观测器在SF型矢量控制系统中的应用

文章提出广义逆矩阵来设计离散型状态观测器的方法,用该法设计的观测器,可对SF型矢量控制系统的负载力矩进行估计,再进行前馈补偿。实验证明,该法可有效改善系统的动态性能。     

一种改进的树形矢量量化编码算法

矢量量化中的一个最严重的问题是在一本码本中搜索最近码字的高计算复杂度。本文在研究树形矢量量化的基础上提出了一种改进的树形矢量量化编码算法。实验结果表明,本文提出的编码算法相对于树形矢量量化算法可大大改善峰值信噪比（ＰＳＮＲ）。     

稳定非线性动力系统周期的计算机分析

提出采用后继函数法来确定非线性动力系统的周期,并编制计算机程序对Ｖａｎ ｄｅｒ Ｐｏｌ方程实例计算周期和绘制相图,结果表明,该方法计算结果准确且实用性强,本方法不但适合于求解保守系统和具 周期解非线性动力系统的周期,也适合于耗散系统和不稳定系统的分析。     

烯烃的分子距离边数矢量(μ)及沸点的估计与预测

基于双键相对于单键键长的大小,提出了烯烃的分子距离边数（ＭＤＥ）矢量（μ矢量）;进而通过多元线性回归方法建立μ矢量与烯烃沸点之间的关系模型并进行预测,计算沸点与实验值之间的均方根误差仅为３．７８Ｋ,相关系数为０．９９９３。     

信号流图的扩展及其应用

将信号流图扩展到状态空间构成所谓向量流图,用公式可实现组合系统规范化;按工答过程绘制信号流图构成所谓过程流图,可对系统组件作深入的分析。     

Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

Research advances in gene therapy for Parkinson's disease

During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade.     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

Construction of High Level Expression Cell Lines of Erythropoietin *

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