孕激素对应激性胃溃疡的保护和治疗作用

目的：探讨孕激素对应激性胃溃疡的保护和治疗作用．方法州、鼠分组。注射生理盐水及不同剂量的孕激素后,束缚加冷水刺激,计算溃疡指数及测定胃酸的pH值;束缚加冷水刺激后,注射生理盐水及孕激素,于应激后即刻、应激后5h、应激后7h计算溃疡指数．结果发现应激前注射孕激素对胃溃疡有明显的预防和保护作用;而溃疡发生后,注射孕激素有较好的治疗作用．     

过期妊娠产妇外周血和羊水雌二醇及孕酮水平的测定

目的 :测定过期妊娠产妇外周血血清和羊水中的雌二醇 (E2 )、孕酮 (P4 )水平 ,探讨E2 、P4水平变化与过期妊娠及宫缩发动的相关性。方法 :放射免疫法测定过期妊娠与足月妊娠产妇 (各2 0例 )羊水和外周血血清E2 、P4 浓度。结果 :1)过期妊娠组产妇外周血血清和羊水P4 水平明显高于足月妊娠组 (2 0 4 13± 4 1 16vs 176 4 6± 2 4 89,t =2 5 86 ,P <0 0 5 ;10 7 0 6± 2 8 6 6vs 71 2 3±13 6 7,t=5 0 4 6 ,P <0 0 1) ,E2 /P4 值明显低于足月妊娠组 (0 78± 0 17vs 0 90± 0 16 ,t=- 2 2 4 7,P<0 0 5 ;0 19± 0 10vs 0 2 6± 0 0 6 ,t=- 2 5 5 2 ,P <0 0 5 ) ,差异有显著性。 2 )临产组与未临产组产妇外周血血清中E2 、P4 水平及E2 /P4 值均无明显差异 (147 98± 12 0 0vs 16 2 0 5± 2 8 38,t =-1 76 2 ,P >0 0 5 ;175 0 2± 2 2 84vs 198 5 2± 4 0 0 8,t =- 2 0 17,P >0 0 5 ;0 88± 0 18vs 0 82±0 17,t=1 136 ,P >0 0 5 ) ;而临产组羊水中的P4 水平低于未临产组 (74 39± 16 73vs 97 0 9± 30 77,t=- 2 5 5 4 ,P <0 0 5 ) ,E2 /P4 值高于未临产组 (0 2 9± 0 0 9vs0 18± 0 0 6 ,t=- 4 4 41,P <0     

STUDY ON REPRODUCTIVE ENDOCRINOLOGY OF HUMAN PLACENTA (Ⅲ)——HORMONAL REGULATION OF PROGESTERONE PRODUCTION BY TROPHOBLAST TISSUE OF FIRST TRIMESTER

The effect of hormones on progesterone secretion by 6-8 week human trophoblast tissue cultured in serum-free medium has been investigated. GnRH at low concentration (10-10-10-8 mol/L) stimulated progesterone secretion, while high dose (10-6-10-5 mol/L) produced inhibitory effect. The progesterone secretion could be significantly decreased by addition of anti-hCG antiserum or monoclonal anti-hCG IgG in a dose- and time-dependent manner. Various concentrations of TRH, PGE2, PGF2α, testosterone and estradiol were found to be ineffective. These data indicate clearly that progesterone production by human trophoblast tissue at early gestation stage is under the modulation of GnRH and hCG.     

Derivative Spectrophotometric Analysis of Oestradiol Esters,Testosterone and Progesterone in Oily Injections

Abstract

The use of derivative and difference-derivative spectrophotometry for the determination of certain drugs in oily injection is presented. The method is illustrated by the determination of oestradiol valerate, oestradiol dipropionate, oestradiol benzoate, testosterone propionate and progesterone in their oily injections. This method is simple, rapid and accurate. The results obtained are reasonably reproducible with a coefficient of variation less than 2%.     

紫草多糖对hCG诱导大鼠黄体细胞分泌功能的影响

采用体外黄体细胞培养,观察中药紫草的水溶成分——紫草多糖对hCG诱导的黄体细胞分泌孕酮影响,并探讨其可能机制。选取未成年雌性SD大鼠,分离黄体细胞,分为基础组和hCG组,分别添加不同浓度紫草多糖,放射免疫检测各组细胞内和培养液中的孕酮(Progesterone,P4)含量;将细胞悬液分为4组:空白对照组、1.50g.L-1紫草多糖组、2×104 U/L hCG组以及hCG(2×104 U/L)+紫草多糖(1.50g.L-1)组,在培养的不同时间点(0、0.5、1、2h时),分别测P4产量,观察紫草多糖对培养黄体细胞作用的起效时间和动力学变化。此外,上述4组培养0.5h后离心分离细胞和培养液,分别测得细胞内和培养液中的cAMP和cGMP含量,将细胞内和培养液中的含量之和作为总量,并计算其cAMP/cGMP比值。结果表明:1.紫草多糖呈剂量依赖性地抑制hCG刺激下黄体细胞P4的生成。2.加大剂量(>3.00g.L-1)紫草多糖亦可抑制基础P4的生成。3.1.50g.L-1紫草多糖组从作用0.5h起,即显著抑制黄体细胞hCG刺激下的P4生成(P<0.01)。4.hCG可明显增加黄体细胞cAMP含量,导致cAMP/cGMP增加。添加1.50g.L-1紫草多糖可增加cAMP、cGMP含量,但cAMP/cGMP比值下降。结论:紫草多糖可直接作用于促性腺激素的靶细胞——黄体细胞,并抑制hCG诱导黄体细胞分泌功能,该抑制作用起效快,并可能通过cGMP介导。     

Investigation of Progesterone Loaded Poly(D,L-Lactide) Microspheres Using TMDSC,SEM and PXRD

Poly(d,l-lactide) microspheres with progesterone loadings of 0, 10, 20, 30 and 50% w/w were manufactured using an interrupted solvent evaporation process. Spherical microspheres with loadings close to the theoretical values were produced. The glass transition of the polymer could be identified by a step change in the heat capacity measured by TMDSC. Progesterone was found to plasticise the glass transition temperature at contents of 20% w/w or less. At a 30% loading, cold crystallisation of progesterone was seen indicating that an amorphous form of the drug was present; these microspheres were found to exhibit a pitted surface. TMDSC of the 50% progesterone samples suggested that most of the drug was present as crystals. This was supported by the SEM and PXRD results. This revised version was published online in July 2006 with corrections to the Cover Date.     

由孕甾-3S,5R,6R,16S,20S-五醇合成黄体酮

首次利用薯蓣皂甙元降解产物, 孕甾-3S,5R,6R,16S,20S-五醇(3)完成了黄体酮的合成. 孕甾-3S,5R,6R,16S,20S-五醇可方便地通过薯蓣皂甙元经由30%双氧水原地产生的过酸氧化降解得到. 它经过缩酮化反应、乙酰化反应和串联的脱缩酮-溴代乙酰化反应被转化成关键合成中间体16R-溴孕甾-3S,5R,6R,20S-四醇-3,6,20-三乙酸酯(6). 化合物6经锌粉还原、C-3乙酰氧基选择性水解氧化反应和C-5羟基消除反应生成6R,20S-二乙酰氧基孕甾-4-烯-3-酮(10). 化合物10经C-6乙酰氧基还原和C-20乙酰氧基水解-氧化生成目标分子黄体酮. 合成经10步反应, 反应总收率达45.1%.     

Shotgun metabolomic profiles in maternal urine identify potential mass spectral markers of abnormal fetal biochemistry – dihydrouracil and progesterone in the metabolism of Down syndrome

In Down syndrome (DS) in particular, the precise cellular mechanisms linking genotype to phenotype is not straightforward despite a clear mapping of the genetic cause. Metabolomic profiling might be more revealing in understanding molecular–cellular mechanisms of inborn errors of metabolism/syndromes than genomics alone and also result in new prenatal screening approaches. The urinary metabolome of 122 maternal urine from women with and without an aneuploid pregnancy (predominantly Down syndrome) were compared by both zwitterionic hydrophilic interaction chromatography (ZIC‐HILIC) and reversed‐phase liquid chromatography (RPLC) coupled to hybrid ion trap time of flight mass spectral analysis. ZIC‐HILIC mass spectrometry resolved 10‐fold more unique molecular ions than RPLC mass spectrometry, of which molecules corresponding to ions of m/z 114.07 and m/z 314.20 showed maternal urinary level changes that significantly coincided with the presence of a DS fetus. The ion of m/z 314.20 was identified as progesterone and m/z 114.07 as dihydrouracil. A metabolomics profiling‐based maternal urinary screening test modelled from this separation data would detect approximately 87 and 60.87% (using HILIC‐MS and RPLC‐MS, respectively) of all DS pregnancies between 9 and 23 weeks of gestation with no false positives. Copyright © 2014 John Wiley & Sons, Ltd.     

Synthesis of 15β, 16β-methyleneprogesterone

15,16-Methyleneprogesterone was produced from 15,16-methylene-3-hydroxyandrost-5-en-17-one with Wittig olefination of 17-ketone by methoxymethylenetriphenylphosphorane as the key stage.Translated fromIzvestiya Akademii Nauk, Seriya Khimicheskaya, No. 1, pp. 203–207, January, 1993.     

High Performance Liquid Chromatographic Determination of Testosterone Propionate,Progesterone and Estradiol Benzoate in Bovine Implants

Abstract

A Method for the determination of testosterone propionate, progesterone, and estradiol benzoate in bovine implants is presented. The method utilizes a Dupont, zorbax DDS analytical column with a mobile phase consisting of methanol, deionized water, and tetrahydrofuran (65:30:15 v/v). Sample preparation consists of of dissolution of the implant, followed by dilution and injection onto the HPLC system. The procedure is accurate, precise, linear, and specific for the implant steroids with quantitation by peak height, using dipenty 1 phthalate as the internal standard.