过江光缆施工技术

以新长铁路为例，从施工前、施工中及施工后的具体情况出发，对过江光缆施工技术进行了详细的说明，就如何减小风险提出了一些建议。     

高速公路路堑边坡稳定模拟分析及治理措施

基于极限平衡理论,针对湖南长沙机场高速公路沿线路堑边坡,分析了边坡的物质成分、结构构造、雨水渗透下岩土渗透性、抗剪强度以及裂隙等对边坡稳定性的影响,并采用快速拉格朗日法对该段边坡进行了数值模拟计算.研究结果表明:该边坡在构造上具有走向与公路轴线基本一致,倾向与坡面一致的特点,同时,上部强风化的泥质粉砂岩裂隙十分发育,岩石具有遇水软化的特征,在雨水的作用下,岩土的抗剪强度急剧降低,导致顺层滑坡;该路堑边坡的稳定性较低.通过对边坡采用锚固的治理措施,既能保持高速公路沿线整体性,又能达到加固的目的.     

超载下预应力CFRP布加固腐蚀钢梁抗弯性能试验

为研究预应力CFRP布加固腐蚀钢梁静载、超载的抗弯性能,进行了7根钢梁的抗弯试验,研究了加固梁在静载及超载下的破坏形态、承载力和刚度.结果表明,加固钢梁可以提高钢梁的承载力与刚度,预应力的存在,可以显著提高CFRP布的利用率;腐蚀程度影响钢梁的刚度与极限承载力,腐蚀程度增加1倍,钢梁的承载力降低50%左右.超载次数增加,钢材发生时效硬化,钢梁刚度可提高13%左右,但承载力有所降低;超载下,预应力的施加可提高钢梁的延性.建立了预应力CFRP布加固腐蚀钢梁的承载力计算公式,理论计算结果与试验结果吻合良好.     

基因重组型枯草芽孢杆菌芽孢表面展示技术与应用

枯草芽孢杆菌是一种好氧型革兰氏阳性益生菌,在营养匮乏的环境下,可形成具有强抗逆性的芽孢。其芽孢独特的结构和生理功能使得芽孢表面展示技术相较于其他表面展示技术具有多种优势,构建的重组芽孢不但易纯化、回收率高,而且安全性好。近年来,枯草芽孢杆菌芽孢表面展示技术得到迅猛发展,已成功利用CotB、CotC、CotG、CotZ、CotA、OxdD、CotE、CotZ、CgeA等多种锚定蛋白将外源蛋白或多肽展示在芽孢表面,并应用于工业化酶、口服疫苗和药物、大分子量多聚体蛋白的生产以及环境污染的生物治理等领域。本文首先介绍了芽孢展示系统整合策略,并重点阐述了基因重组型枯草芽孢杆菌芽孢展示技术中涉及的锚定蛋白以及该技术在各领域的应用与展望。     

Alternating-current losses in two-layer superconducting cables consisting of second-generation superconductors coated by U-shaped ferromagnetic materials

Alternating-current losses in a two-layer superconducting cable, each layer being composed of 15 closely-spaced rectangular wires made up of second-generation superconductors when the ends of wires are coated by either a non-magnetic or strong ferromagnetic material having a U profile is numerically investigated. Computations are carried out through the finite-element method. The alternating-current losses do not increase significantly if the relative permeability of the coating is increased three orders of magnitude, provided that the current amplitude is less than half of the critical current in a superconducting wire. However, the losses are much higher for ferromagnetic coating if the amplitude of the applied current oscillating at 50 Hz is close to the critical current. The ferromagnetic coating is seen to accumulate the magnetic field lines normally on its surfaces, while the field lines are parallel to the long axes of the wires, leading to more significant flux penetration in the coated regions. This facilitates a uniform low-loss current flow in the uncoated regions of the wires. In contrast, coating with a non-magnetic material gives rise to a considerably smaller current flow in the uncoated regions, whereas the low-loss flow is maintained in the coated regions. Moreover, the current flows in opposite directions in the coated and uncoated regions, where the direction in each region is converse for the two materials.     

特殊条件下巷道锚索支护结构与参数研究

现场采集五家沟矿5#煤层及顶板煤岩试样,实验室测定其力学参数。运用数值模拟方法,研究简式桁架锚索与单体锚索不同组合结构围岩控制效果,得出厚煤顶块裂煤巷简式桁架锚索关键支护参数。结合工程类比法设计5#煤层辅运大巷简式桁架锚索与单体锚索"交错布置"组合支护方案。实测顶底板相对移近量152 mm、两帮移近量125 mm,围岩控制效果良好。     

一起400V户外电缆短路事故的分析与处理

针对一起400 V户外电力电缆短路故障,通过现场查勘及对事故原因三阶展开分析、运用鱼骨图揭示造成事故的各种因素,并结合矩阵图提出改进办法和应吸取的经验教训。     

侯西铁路线K81＋061立交桥梁体后张法预应力筋伸长值偏差探讨与计算机辅助计算

结合工程实例,从施工材料和施工技术、混凝土弹性模量增长滞后、设计伸长值等方面,对后张法预应力筋伸长值超限问题进行了系统的论述,最终采用理论计算的手段解决了该问题,并针对理论计算的难度及特点提出了计算机高级语言辅助计算的解决方法与思路。     

基于联合分步APIT定位算法的改进

本文研究了无线传感网络( Wireless Sensor Network,WSNs)的节点定位问题,并针对APIT由于锚节点在低密度环境下的节点误判和节点失效等问题给出了改进,在APICT定位算法的基础提出了联合分步定位算法UNION-APICT(Union Approximate Point-In-Circumcircle Test),该算法是结合连通性的测距技术,RSSI测距技术以及质心定位和APICT等技术,来联合解决对未知节点定位问题。通过仿真实验结果表明,改进后的UNION-APICT在APICT算法的基础之上平均定位误差减少了10%-25%,定位性能有了明显的提升;随着通信半径R和最大探测距离rmax的增加,定位误差也在逐渐减小,该算法较APIT和APICT定位算法在锚节点密度、节点覆盖率和定位精度上都有所提高。     

Semisynthesis of Functional Glycosylphosphatidylinositol‐Anchored Proteins

Glypiation is a common posttranslational modification of eukaryotic proteins involving the attachment of a glycosylphosphatidylinositol (GPI) glycolipid. GPIs contain a conserved phosphoglycan that is modified in a cell‐ and tissue‐specific manner. GPI complexity suggests roles in biological processes and effects on the attached protein, but the difficulties to get homogeneous material have hindered studies. We disclose a one‐pot intein‐mediated ligation (OPL) to obtain GPI‐anchored proteins. The strategy enables the glypiation of folded and denatured proteins with a natural linkage to the glycolipid. Using the strategy, glypiated eGFP, Thy1, and the Plasmodium berghei protein MSP1₁₉ were prepared. Glypiation did not alter the structure of eGFP and MSP1₁₉ proteins in solution, but it induced a strong pro‐inflammatory response in vitro. The strategy provides access to glypiated proteins to elucidate the activity of this modification and for use as vaccine candidates against parasitic infections.