Two heptadentate Co(III) and Mn(III) complexes with partially deprotonated cyclen derivative bearing four hydroxypropyl pendants: structure,DNA binding and DNA cleavage

Two new complexes, (Co^III)₂(H₃L^?)₂(0.5H₂O)₂(ClO₄)₄ (I) and (Mn^III)₂(H₃L^?)₂(0.5H₂O)₂ (ClO₄)₄ (II), were synthesized and crystallographically characterized [H₄L = 1,4,7,10‐tetra‐(2‐hydroxypropyl)‐l,4,7,10‐tetraazacyclododecane] using electrospray ionization mass spectrometry and X‐ray photoelectron spectrometery. The characterizations confirmed that the valences of the metal ions increased from divalent to trivalent due to deprotonation of one OH group (H₄L was in the form of H₃L^?). Owing to the instability of Co(III) and Mn(III) in both air and in solution, they preferred to exist in divalent form. The two heptadentate complexes are extraordinary in that the chiral pendants of the complexes are different in configuration. Spectroscopic studies, viscosity measurements, thermal denaturation experiments and circular dichroism spectra demonstrated that the complexes were prone to interact with DNA by groove binding. At micromolar concentrations and under physiological conditions, the two complexes were able to oxidatively cleave the supercoiled pUC19 plasmid DNA into its nicked and linear forms. Mechanistic studies using various additives suggest the complexes had structures different from those of other inorganic complexes. These are the first reported inorganic complexes not containing planar aromatic ligands and yet binding at the major groove. Copyright © 2012 John Wiley & Sons, Ltd.     

前缀码与极大前缀码的一种新刻画

在语言图Γ(X*)概念的基础上,用新引入的语言竹竿l(X*)和语言竹竿集L(X*)的概念形象地刻画了前缀码与极大前缀码:A是前缀码l(X*)∈L(X*),S_l_((X*))∩A或为单点集或为Φ;A是极大前缀码l(X*)∈L(X*),i)S_(l(X*))∩A或为单点集或为Φ.ii)S_(l(X*))∩A=Φ■u∈S_(l(X*)),■a∈A,ω∈X~+使a=uw.     

Wound Healing Attributes of Polyelectrolyte Multilayers Prepared with Multi‐l‐arginyl‐poly‐l‐aspartate Pairing with Hyaluronic Acid and γ‐Polyglutamic Acid

Biodegradable multi‐l ‐arginyl‐poly‐l ‐aspartate (MAPA), more commonly cyanophycin, prepared with recombinant Escherichia coli contains a polyaspartate backbone with lysine and arginine as side chains. Two assemblies of polyelectrolyte multilayers (PEMs) are fabricated at three different concentration ratios of insoluble MAPA (iMAPA) with hyaluronic acid (iMAPA/HA) and with γ‐polyglutamic acid (iMAPA/γ‐PGA), respectively, utilizing a layer‐by‐layer approach. Both films with iMAPA and its counterpart, HA or γ‐PGA, as the terminal layer are prepared to assess the effect on film roughness, cell growth, and cell migration. iMAPA incorporation is higher for a higher concentration of the anionic polymer due to better charge interaction. The iMAPA/HA films when compared to iMAPA/γ‐PGA multilayers show least roughness. The growth rates of L929 fibroblast cells on the PEMs are similar to those on glass substrate, with no supplementary effect of the terminal layer. However, the migration rates of L929 cells increase for all PEMs. γ‐PGA incorporated films impart 50% enhancement to the cell migration after 12 h of culture as compared to the untreated glass, and the smooth films containing HA display a maximum 82% improvement. The results present the use of iMAPA to construct a new layer‐by‐layer system of polyelectrolyte biopolymers with a potential application in wound dressing.     

基于微博文本的个性化兴趣关注点及情绪变迁趋势研究

随着社交网络的快速发展,人们可利用微博平台发表、分享自己的观点以及抒发某种情绪,进而产生了大量针对不同话题的博文和情绪信息,但传统的文本挖掘算法在处理这些短小且具富含个性化情感信息的微博文本方面有所欠缺。在此提出一种基于微博文本的特征权重计算方法,可据此得到博主在不同时间段的关注点,通过情绪分类,分析用户在不同时间段内的情绪变迁情况。实验结果证明此方法具有一定的可行性。     

基于6LoWPAN６的PRL路由协议研究

鲁迅对日本文化和日本国民性的关注更多的是从中国国民性批判的角度来进行的,他的日本认识包含两个层面:一是作为国民性改造过程中的一个参照物的日本,他过多地强调日本民族的优点而少谈日本民族的缺点,就在于他是以日本民族的优点作为救治本民族缺点的"药",日本民族的优点的选择是出于本民族现实需要的目的;二是作为独立民族文化的日本,他摆脱了中日冲突背景下的激进和狭隘的民族主义,以冷静的态度进行中日文化的交流,以期促成民族间的理解和友好。     

农业院校学生农村就业的利与弊

在当今社会竞争如此激烈的大环境下,高校毕业生的规模又如此的庞大,就业的压力越来越大,有一种呼声:农业院校的毕业生到农村就业是唯一途径,这种声音普遍受到社会和人们的关注。其实现在的农业院校并非以前仅仅是农业专业,因经济全球化的快速发展让农业院校在专业方向的建设上也开始多元化。再且,由于现在的毕业生都是80后、90后,去农村就业有利有弊,所以农村并不是农业院校毕业生的唯一出路。     

大学生马基雅维利主义倾向探微

个体的马基雅维利主义倾向影响其对人际交往的态度与实践。文章采用西方成熟量表,通过对南京某高校274名大学生样本研究结果发现:多数人的马基雅维利主义水平比较低,约三分之一强的人属于高马基雅维利主义者;男生要比女生表现出较高的马氏水平;非商科学生比商科学生表现出较高的水平;三个年级中,大一学生的马氏水平最高;在所有学生中,城市大学生的马氏水平最高,来自农村与小城镇大学生的马氏水平几乎相同。     

Preparation and characterization of a polystyrene/bovine serum albumin nanoparticle‐coated capillary for chiral separation using open‐tubular capillary electrochromatography

Polystyrene (PS) nanoparticles coated by BSA, hereafter denoted as PS/BSA, were prepared and chemically immobilized for the first time onto a capillary inner wall for open‐tubular CEC (OTCEC). EOF and scanning electron micrography were used to characterize the prepared nanoparticle‐coated capillaries. To investigate the performance of the prepared columns in OTCEC, chiral separation of d ,l ‐tryptophan (dl ‐Trp) was performed in monolayer BSA‐modified capillary and PS/BSA nanoparticle‐coated columns. The results indicated that the nanoparticle‐modified column afforded a higher resolution compared with the monolayer type. Rapid enantioseparation of dl ‐Trp (within 3 min) was achieved with the PS/BSA‐immobilized column using an electroosmotic pump‐assisted CEC. Enantiomer separations of other compounds like dl ‐tyrosine and warfarin were also achieved with the column. Besides, run‐to‐run and column‐to‐column repeatabilities of the PS/BSA‐coated column in the chiral separation were systematically introduced.