Rearrangements of microtubule cytoskeleton in stomatal closure of Arabidopsis induced by nitric oxide

NO (nitric oxide), known as a key signal molecule in plant, plays important roles in regulation of stomatal movement. In this study, microtubule dynamics and its possible mechanism in the NO signal pathway were investigated. The results were as follows: (i) In vivo stomatal aperture assays revealed that both vinblastine (microtubule-disrupting drug) and SNP (exogenous NO donor) prevented stomatal opening in the light, and vinblastine even could enhance the inhibitory effect of SNP, whereas taxol (a microtubule-stabilizing agent) was able to reduce this effect; (ii) microtubules in the opening Arabi- dopsis guard cells expressing GFP:α-tubulin-6 (AtGFP:α-tubulin-6) were organized in parallel, straight and dense bundles, radiating from the ventral side to the dorsal side, and most of them were localized perpendicularly to the ventral wall; (iii) under the same environmental conditions, treated with SNP for 30 min, the radial arrays of microtubules in guard cells began to break down, twisted partially and be- came oblique or exhibited a random pattern; (iv) furthermore, the involvement of cytosolic Ca2 in this event was tested. Stomatal aperture assays revealed that BAPTA-AM (a chelator of Ca2 ) greatly sup- pressed the effect of NO on stomatal closure; however, it did not show the same function on stomatal closure induced by vinblastine. When BAPTA-AM was added to the SNP-pretreated solution, the SNP-induced disordered microtubulue cytoskeleton in guard cells underwent rearrangement in a time-dependent manner. After 30 min of treatment with BAPTA-AM, the cortical microtubules resumed the original radial distribution, almost the same as the control. All this indicates that NO may promote rearrangement of microtubule cytoskeleton via elevation of [Ca2 ]cyt (free Ca2 concentration in the cy- toplasm), finally leading to stomatal closure.     

Fabrication and Tribological Properties of Pb Nanoparticles

In this paper, we describe a surfactant-assisted solution dispersion method to obtain metal nanoparticles, which involves dispersing and stabilizing metal droplets in an appropriate solvent. This method has been successfully used to prepare Pb nanoparticles from bulk Pb. The X-ray powder diffraction and transmission electron microscopy investigations show the formation of Pb nanoparticles possessing the same crystal structure as bulk metal and an average particle diameter of 40 nm. Thermal analysis indicates that Pb nanoparticles have organic shell, which is in agreement with the excellent oil-solubility. In addition, the tribological properties of Pb nanoparticles as additive in oil are discussed.     

竞赛用投篮自动机器人机构运动分析

为参加全国大学生机器人电视大赛，研制了投篮自动机器人。根据机器人的工作任务和环境，确定了投篮自动机器人的主要技术指标，对机器人在投球过程中机械臂的运动做了分析计算，并对完成投球动作的随机事件做了概率分析，为机器人的设计及控制系统的设计提供了依据。     

铁酸盐纳米微粒的制备及催化性能

采用溶胶－凝胶法制备了含Zn、Ni、Co的铁酸盐纳米微粒催化剂，运用DTA－TG、IR、XRD以及BET比表面测试等手段对所制备的样品进行了表征，同时，考察了铁酸盐对二氧化碳选择性氧化乙苯制苯乙烯的催化性能。结果表明：制备的铁酸盐均为尖晶石型晶体，粒了的比表面积为31．9－36．3m^2/g，晶粒大小约为30－35nm，在由二氧化碳选择性氧化乙苯制苯乙烯的反应中表现出较好的催化活性。     

甲基磺酸铜制备的绿色工艺与动力学

甲基磺酸(ＭＳＡ)及其盐类能改善镀层性能,并可实现无氰电镀[1-4],本文采用空气氧化法直接由紫Ｃｕ与ＭＳＡ水溶液反应生产(ＣＨ3ＳＯ3)2Ｃｕ·5Ｈ2Ｏ,达到了工艺绿色化的要求。1　实验部分1 1　试剂与仪器70%ＭＳＡ(宝鸡科隆化工有限公司);99 9%紫铜片(沈阳冶炼厂);氧气(宝鸡有色金属加工厂氧气分厂);其余试剂均为分析纯试剂。ＡＡ7003Ａ原子吸收光谱仪(北京市东西电子技术研究所);ＰＩＣ-8型离子色谱仪(青岛普仁仪器有限公司);超级恒温槽。1 2　实验将500ｍｌ三口磨口烧瓶装于超级恒温槽中,装温度计、鼓气管,第三只磨口连续接装3支磨…     

徒手体操术语与定位法舞谱的比较研究

徒手体操术语与定位法舞谱作为描述形体动作的工具，它们在理论体系上是不同的，本文通过比较，总结了构成它们各自理论体系的基本原理、描述依据与构成因素等，并指出二者在运用时的准确性、简炼性、易懂性的差异．     

Influence of ultrasound on the preparation of ruthenium catalysts supported on alumina

Ultrasound waves are commonly used to clean or to erode the surface of solid materials thanks to the strength of the jets of solvent, hitting the surface itself, created by the collapse of the liquid phase. In the present work, ultrasound was used to obtain highly dispersed ruthenium on alumina catalysts. The samples were characterized with different techniques to study the influence of the sonication on both the metal crystallites and the support.     

受断层影响地表移动规律的研究

在建立了地表移动观测站及现场观测的基础上，对云盖山煤矿地表移动观测站观测资料进行系统的计算和分析，得出了该区域地质采矿条件下地表移动变形规律及有关参数。并研究分析了该区域在断层影响下地表移动和变形规律的特殊性及其形成原因。为本矿区及类似条件下“三下“采煤技术的研究提供了可靠的理论依据。     

电镜样品的激光镀膜技术

非导电的样品用扫描电镜进行观测时需要对样品进行镀膜处理以获得清晰的图像, 本文介绍应用脉冲激光沉积(PLD)技术对非导电样品的表面进行镀膜处理的方法和实验装置及实验结果.