Development and validation of a highly sensitive LC‐MS/MS‐ESI method for the determination of bicalutamide in mouse plasma: application to a pharmacokinetic study

A highly sensitive, rapid assay method has been developed and validated for the estimation of bicalutamide in mouse plasma using liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative‐ion mode. The assay procedure involves extraction of bicalutamide and tolbutamide (internal standard, IS) from mouse plasma with a simple protein precipitation method. Chromatographic separation was achieved using an isocratic mobile phase (0.2% formic acid:acetonitrile, 35:65, v/v) at a flow rate of 0.5 mL/min on an Atlantis dC₁₈ column (maintained at 40 ± 1°C) with a total run time of 3.0 min. The MS/MS ion transitions monitored were m/z 428.9 → 254.7 for bicalutamide and m/z 269.0 → 169.6 for IS. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. The lower limit of quantitation achieved was 1.04 ng/mL and the linearity range extended from 1.04 to 1877 ng/mL. The intra‐ and inter‐day precisions were in the ranges of 0.49–4.68 and 2.62–4.15, respectively. Copyright © 2012 John Wiley & Sons, Ltd.     

采用IC传感器的相对湿度测量

本文在比较各种相对湿度测量方法及传感器的基础上，结合实例介绍了集成湿度传感器的特性及使用方法，指出使用条件的影响因素及补偿措施，给出了带有温度效应补偿作用的相对湿度检测线路，并提供了检测软件计算的具体表达式，分段实施的对比实验结果，以及检测过程的外界条件限制。     

百麦外用中药制剂对小鼠乳腺增生的实验研究

目的:观察外用中药制剂对小鼠乳腺增生的预防作用并探讨其机理。方法:采用苯甲酸雌二醇腹腔注射造模,分对照组、模型组与预防组。模型组与预防组腹腔注射苯甲酸雌二醇,隔天1次,并且预防组小鼠乳房涂抹百麦外用中药制剂,每天1次,共30天;对照组小鼠腹腔注射生理盐水,隔天1次,共30 d。测定各组小鼠体重变化,乳晕及乳头直径变化,血清雌激素(E2)、孕激素(P)指标,并取小鼠第二对乳房组织病理切片检查。结果:预防组与模型组小鼠体重、乳晕及乳头直径比较均有显著性差异(P0.05);预防组与模型组比较,E2水平明显降低(P0.05),P水平明显升高(P0.05);预防组小鼠增生的乳腺小叶、腺泡数、腺泡腔直径、导管直径以及胞浆面积较模型组降低。结论:百麦外用中药制剂具有预防乳腺增生的作用,其机理可能是通过抑制血清雌二醇水平而实现。     

Used digital speckle correlation method to realize orientation function of optical mouse

Used digital speckle correlation method (DSCM) to realize orientation function of optical mouse is researched. The experiment is designed to obtain the dynamic speckle patterns which are shot by CCD, then the DSCM is used to process the sequential images and also the experiment is simulated. The experimental results show the DSCM can confirm orientation function of the optical mouse, and have a good agreement with the simulation results, and the resolution we obtained is higher than the resolution of mouse on the market.     

用TOC仪分析连二亚硫酸钠中碳酸钠含量

本方法介绍了采用TOC(总有机碳)分析仪测定连二亚硫酸钠(俗称保险粉)中的IC(无机碳)即碳酸钠含量,比目前使用的容量分析法测定更接近所含碳酸钠的真实值,对各种保险粉中的碳酸钠含量的测定均适用.尤其在保险粉生产控制分析中,判定是否因碳酸钠加入过量而造成保险粉质量不合格具有公正性、可比性.     

太湖猪淋巴细胞和小鼠骨髓瘤细胞杂交细胞的建立

以我国优良猪种小梅山（属太湖猪种）的脾淋巴细胞和小鼠骨髓瘤细胞（ＳＰ２／０）作亲本，经聚乙二醇（ＰＥＧ）促融，ＨＡＴ培养基选择培养，先后得到２５株杂种细胞，并对杂种细胞进行染色体分析和同工酶电泳等研究。结果显示，杂种细胞系含有全部小鼠骨髓瘤细胞染色体，而猪染色体丢失迅速，仅保留猪的４号、５号、７￣１０号、１２号、１３号、１５号和１７号等部分染色体，杂种细胞内能检测到全部小鼠同工酶活性，但仅能检测到     

A system of treatment for correlation signals

Correlator is the critical equipment of the correlational technique. A kind of correlator with double count systems, composed of IC (Integrate Circuit) chips is developed. It is linked to a computer through interface. Using this system, the autocorrelation function of a digital signal (trains of pulses) is obtained and sent to a computer for further treatment. Supported by the National Natural Science Foundation of China and National Educational Committee of China (No. 19674042) Zhang Shihai: born in 1973. Master graduate student     

微机控制的巨型石英钟设计

给出了种利用单片机微机８０３１和集成实时时钟芯片ＭＣ１４６８１８控制的巨型石英钟的电路结构图以及部分程序流图。     

C60基Ebselen衍生物：合成及尤其对鼠胸腺细胞增强保护效应

丙二酸酯衍生物2用2-氯硒基苯甲酰氯和2-(2-胺基乙氧基)乙醇为原料，经三步合成制得。2与C₆₀的Bingel环加成反应制得C₆₀基Ebselen衍生物3，产率为42% (基于已反应的C₆₀)，¹HNMR、¹³CNMR、IR、FAB-MS和元素分析鉴定了其结构。为了证实3对鼠胸腺细胞的活力具有增强效应，C₆₀衍生物4和2被选择，并采用与3相同的方法来处理鼠胸腺细胞，结果显示：3处理组的MTT(OD)值(0.335 ± 0.021)高于4处理组(MTT(OD): 0.283 ± 0.031)和2处理组(MTT(OD):0.247 ± 0.025)，表明3在促进鼠胸腺细胞活力方面优于2 和 4。