诊断超声波对人中孕胎儿大脑皮质神经细胞超微结构影响的初步研究

目的；研究B超定点扫描是否对人中孕胎儿大脑皮质神经细胞超微结构造成影响。方法：将8名健康因避孕失败而自愿要求引产的单胎中孕妊娠（18～25周）的妇女随机分成两组，每组各4例，行水囊引产术，对照组在引产前不作B眼扫描；实验组在引产前30min用频率3.5MHz70%的输出B超（I-SPTA=124.1mW/cm^2）经孕双腹部定点扫描胎儿大脑颞叶皮质10min，引产术后胎儿娩出即取大脑颞叶皮质作透射电镜标本的常规制备与观察。结果：与对照组相比，B超定点扫描10min，胎儿大脑皮质神经细胞的主要变化：（1）细胞核染色质分布不均，成团块状浓缩或边聚；（2）核周质（胞质）内糖原与游离核糖体稀疏；（3）部分线粒体肿胀，呈空泡化；（4）次级溶酶体多见。结论：B超定点扫描10min，胎儿大脑皮质细胞开始有变性改变，由于受损神经细胞不易再生，因此，B超用于产前胎儿颅脑检查尤其早，中孕检查时不应超过10min为宜。     

指纹识别式鼠标

该产品开发的目的是将具有唯一性、稳定性的人体生物特征如指纹等用于计算机用户的身份识别，以加强存储在计算机中重要信息的安全。其核心是现场采集的指纹特征与已登记、存储在计算机中的指纹通过可靠的匹配算法，进行一比一的对比来验证用户身份。根据鼠标内部较大空间、贴片式指纹识别芯片及微处理器体积很小的特点，将三者集成为集鼠标与用户身份识别功能于一体的“指纹识别式鼠标”，既方便快捷、又唯一可靠。其开发主要包括硬件设备及驱动程序、系统应用软件、网络安全认证中心建立等。     

IC-CIRCOX:啤酒厂的废水处理新工艺

为了改善我国啤酒行业的废水处理状况，对珠江啤酒厂进行了实地调研，并参照上海富士达啤酒有限公司、沈阳华润雪花啤酒有限公司等几家有代表性地企业的废水处理工程实例，提出IC－CIRCOX处理工艺值得推广。     

二氧化硫对小鼠骨髓细胞微核的诱发及沙棘油的防护作用

研究了大气中二氧化硫 (SO2 )污染物的遗传毒理效应。采用 SO2 吸入染毒法 ,模拟 SO2 大气污染 ,从整体水平直接对短期与长期 SO2 吸入诱发小鼠骨髓嗜多染红细胞 (PCE)形成微核 (MN)的效应及沙棘油的防护作用进行了研究。结果表明 :(1)短期与长期 SO2 吸入均可引起小鼠骨髓 PCE细胞微核率和微核细胞率显著升高 ,且有明确的剂量 -效应关系 ;(2 )随着吸入的 SO2 浓度的增高 ,不仅单微核细胞率 ,而且双微核细胞率均显著升高 ;(3) SO2对阳性致突变剂乌拉坦诱发微核的作用有显著抑制效应 ;(4 )沙棘油对 SO2 诱发 PCE微核的效应有防护作用。从此可以结论 :SO2 空气污染物是哺乳类细胞染色体断裂剂和基因毒性因子、SO2 与阳性致突变剂的联合作用是复杂的 ,某些天然物质对 SO2 的毒效应有防护作用     

补充谷氨酰胺对运动小鼠谷氨酰胺代谢的观察

文章研究大强度长时间运动情况下,补充谷氨酰胺(Glutamine ,Gln)对血浆Gln、骨骼肌Gln合成酶和谷氨酰胺酶活性变化的影响,证明大强度运动时,经补充Gln能有效地提高血浆、骨骼肌Gln浓度,维持小鼠在运动中的Gln正常水平。     

D574集成稳压器二次击穿的研究

本文分析了Ｄ５７４集成稳压器热二次击穿和电二次击穿的机理，详细论述了发生这两类二次击穿的条件和原因。     

Molecular genetic basis for the rhino mouse from Chinese KM subcolony

Both the rhino mouse and hairless mouse resulted from hairless gene mutation, but they show different phenotypes of skin physiology. The rhino mouse has more similar histological characters to human papular alopecia.Therefore rhino mouse is a good experimental animal model for human papular alopecia. This study reports a hairless mouse named rhino KIZ, arose from KM colony in Kunming Institue of Zoology, by systematic studies on morphology,skin histopathology, gene sequence, pedigree and protein domain analysis. The results demonstrate that a C-to-T transition in exon 11 of hr gene (The mutant gene has been applied for a Chinese patent (patent No. 03135280)) results in the rhino KIZ. The rhino KIZ with clear genetic mechanism will be a useful animal model.     

Topography of acoustic response characteristics in the midbrain inferior colliculus of Kunming mouse

Topography of acoustic response characteristics of the midbrain inferior colliculus(IC)of the Kunming mouse was studied by using extracellular recording techniques.The characteristic frequency(CF)range represented in the different divisions of the IC differed markedly:4-15 kHz in the dorsal cortex(DC),10-70 kHz in the central nucleus(CN),and 4-35kHz in the external cortex(EC).The CF in the CN increased from dorsal and lateral to ventral and medial,higher CFs represented at its ventromedial part and lower CFs at its dorsal part.The isofrequency contours of CFs were incurvate.Minimum thresholds(MT)of the auditory neurons in DC and the central part of CN were lower(about 10dB SPL),but considerably higher in the dorsal and ventral region of EC.Results suggest that each of the divisions in the mouse IC may have different auditory functions.     

Expression of recombinant human lysozyme in the milk of transgenic mice

Human lysozyme is a 130-aa (amino acid) alkaline polypeptide, and has both anti-bacterial and anti-viral properties which make it an important component of human natural immunity system. As a first step toward the ultimate goal of improving the anti-bacterial properties of bovine and ovine milk, a transgenic mouse that contains the genomic DNA sequence of the human lysozme gene has been generated for the first time. From 83 mice generated by microinjection, a total of 6 positive transgenic mice were identified by PCR and Southern blot. F1 mice positive for transgene in lines were also detected by PCR. This shows that transgene could be transmitted from founder transgenic mice to their offspring. Recombinant human lysozyme (rHlys) was found in the whey of 3 female positive transgenic mice by Western blot. The highest concentration of rHlys for transgenic mice was 0.2mg/mL. The antibacterial activity of the whey for transgenic mice was highly enhanced up to 0.4 times as much as that of human, while that of non-transgenic mouse was very low. Although the lysozyme activity of transgenic mice is still lower than that of human, the rHlys exhibits the same specific activity as that of human lysozyme. It provides a strong basis for further studies into the possible application of rHlys express in mammary gland.