Use of zwitterionic buffer additives to improve the separation of proteins in capillary zone electrophoresis

In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are evaluated in the separation of acidic, neutral, and basic proteins, including their effect on protein efficiency, mobility, separation, and resolution; the difference between the effects of the different additives are also highlighted. The method has proved to be a possible means of reducing protein adsorption, especially for basic proteins.     

断裂带中岩石硅化的微束研究

断裂带中岩石的硅化现象,通常称之为硅化岩或硅化破碎带,即经动力变质作用的断层岩,通过微束技术对其观察,它们显示出下列特征:在扫描电镜(SEM)下,硅化物质表现为细脉状,而在偏光里微镜下则表现为浸染状;对硅化岩进行组构观察,可见压扁 S 面和剪切 S 面;在透射电镜(TEM)下,可进一步区别不同的位错现象;断裂带岩石的硅化现象是受组成岩石的化学元素的构造地球化学作用所制约的,包括动力分异和化学亲和作用,动热变质作用和退化变质作用,氧化和还原作用以及脱水和水解作用。     

Characterization of strongly interacted multiple cracks in an infinite plate

Based on the Kachanov method and the alternating iteration technique, a new method is proposed to deal with the problem of the strongly interacted multiple cracks in an infinite plate. Unlike the Kachanov method which neglects the interaction of the tractions of the non-uniform components, the tractions of the non-uniform components on the surfaces of cracks are considered through the alternating technique. The accuracy and efficiency of present method are validated by comparing the results of two collinear and two parallel overlapped open the cracks obtained by the present method with those of the exact solutions, the results of the Kachanov method and the alternating iteration technique. Applications of present method in solving sliding close crack problems and evaluating the plastic zones demonstrate the versatility of present method.     

辽宁西北地区主要生态环境问题及其整治对策

辽宁西北地区的北部处于中国东部森林区向西部草原区的过渡带上.也是由暖温带半湿润气候向冷温带半干旱气候过渡、农业向牧业过渡的交错带.这一过渡带是中国一级生态敏感带的一部分.近年来,由于气候逐渐趋于暖旱化和人为的不合理开发,这一地区的生态环境逐渐恶化.列出了辽宁西北地区的7个主要生态环境问题,并相应的提出了8个生态环境整治对策.     

Synthesis and studies of antibacterial activity of pongaglabol

Pongaglabol [8-hydroxy-5-phenyl-furo[2,3-h]benzo(b)pyran-7-one] was synthesized and tested for antibacterial effects againstShigella dysenteriae, Salmonella typhi, Streptococcus β-haemolyticus andStaphylococcus aureus. The synthesized compounds were characterized using UV, IR and¹H NMR spectral data     

Thermoelastoplastic deformation of a thick-walled cylinder with a radial crack

The thermoelastoplastic fracture mechanics problem of a thick-walled cylinder subjected to internal pressure and a nonuniform temperature field is solved by the method of elastic solutions combined with the finite-element method. The correctness of the solution is provided by using the Barenblatt crack model, in which the stress and strain fields are regular. The elastoplastic problem of a cracked cylinder subjected to internal pressure and a nonuniform temperature field are solved. The calculation results are compared with available data. __________ Translated from Prikladnaya Mekhanika i Tekhnicheskaya Fizika, Vol. 49, No. 3, pp. 173–183, May–June, 2008.     

Simultaneous determination of cloramphenicol, salicylic acid and resorcinol by capillary zone electrophoresis and its application to pharmaceutical dosage forms

This study demonstrates the separation of active ingredients in acne formulations (salicylic acid, cloramphenicol and resorcinol in presence of azulene) by capillary zone electrophoresis. Factors affecting their separations were the buffer pH and concentration, applied voltage, sample preparation, and presence of additives. Optimun results were obtained with a 50 mM sodium tetraborate-50 mM sodium phosphate, pH 9.0. The carrier electrolyte gave baseline separation with good resolution, short migration times (<6 min), great reproducibility and accuracy. Calibration plots were linear over at least three orders of magnitude of analyte concentrations, the lower limits of detection being within the range 0.39-1.25 μg ml⁻¹. The procedure was fast and reliable and commercial pharmaceuticals could be analysed without prior sample clean-up procedure.     

Determination of contact stresses in problems on bending of a package of transversely isotropic bars

A mechanomathematical model for bending of packages of transversely isotropic bars of rectangular cross section is proposed. Adhesion, slippage, and separation zones between the bars are considered. The resolving equations for deflections and tangential displacements are supplemented with a system of linear differential equations for determining the normal and tangential contact stresses, and boundary conditions are formulated. A scheme for analytical solution of two contact problems—a package under the action of a distributed load and a round stamp—is considered. For these packages, a transition is performed from the initial system of differential equations for determining the contact stresses, where the unknown functions are interrelated by recurrent relationships, to one linear differential equation of fourth order and then to a system of linear algebraic equations. This transition allows us to integrate the initial system and get expressions for the contact stresses.Translated from Mekhanika Kompozitnykh Materialov, Vol. 40, No. 6, pp. 761–778, November–December, 2004.     

Security Applications of Optical Face Recognition System: Access Control in E-Learning

The educational system has gradually shifted from a face-to-face to an e-learning system, which has become prevalent in advanced countries with the advance of information technology, and connection of global networks. Accordingly, a growing demand is emerging for more reliable individual certification with technical precision in order to measure and record learning achievements and credentials of participants. The present system has a limit in terms of registration capacity, therefore, its accuracy has often been questioned. Against this background, an individual certification system is proposed particularly for access control in e-learning. Under our proposed system, a compact optical correlator for facial recognition is employed. This correlator was previously tested for of biometrics authentication accuracy and proved highly reliable, having recorded remarkably low error rates (below 1%). The recorded error rate is sufficiently robust that the system itself can be regarded as a valid and practical viable attestation system.     

旧式大型工具显微镜数显化

大型工具显微镜是用途较广的精密长度计量仪器,主要用于测量螺纹中径、圆柱的直径等。目前实验室和工厂现有的仪器属50年代生产的老型号产品,用目视螺旋测微鼓上的刻度读数,人工记录和处理数据,测量时费时费工。针对大型工具显微镜数显化的问题,阐述了光栅、光电显微镜、单片机、新型传感器在数显上的应用。利用新技术和新型传感器改造老设备,使其实现数字化和智能化,以提高测量效率和测量精度。改造的结果表明,改造后的仪器测量效率大大提高,精度可提高一个数量级。