N-乙酰氨基葡萄糖对绛红小单孢菌合成庆大霉素的影响

研究了N-乙酰氨基葡萄糖对绛红小单孢菌产素的影响,实验探索了N-乙酰氨基葡萄糖添加工艺,在250mL摇瓶发酵过程中培养48h,60h分别添加0.05%,0.15%N-乙酰氨基葡萄糖可使庆大霉素生测效价达2 030U/mL,较对照1 228U/mL提高了65%以上(三次平均基本一致);5L发酵罐试产三批次发酵终结,生测效价平均为1 727U/mL(厡工艺1 097U/mL)提高了57%,效果十分明显.同时测定了发酵过程中菌体代谢各种参数的变化,证明了N-乙酰氨基葡萄糖在绛红小单孢菌生物合成中的促进作用.本方法操作简单,基本不改变原工艺,生产成本低廉,对提高庆大霉素产量效果非常明显.     

The Effect of Bio-Synthesized Silver Nanoparticles on Germination,Early Seedling Development,and Metabolome of Wheat (Triticum aestivum L.)

Changes in the metabolome of germinating seeds and seedlings caused by metal nanoparticles are poorly understood. In the present study, the effects of bio-synthesized silver nanoparticles ((Bio)Ag NPs) on grains germination, early seedlings development, and metabolic profiles of roots, coleoptile, and endosperm of wheat were analyzed. Grains germinated well in (Bio)Ag NPs suspensions at the concentration in the range 10–40 mg/L. However, the growth of coleoptile was inhibited by 25%, regardless of (Bio)Ag NPs concentration tested, whereas the growth of roots gradually slowed down along with the increasing concentration of (Bio)Ag NPs. The deleterious effect of Ag NPs on roots was manifested by their shortening, thickening, browning of roots tips, epidermal cell death, progression from apical meristem up to root hairs zone, and the inhibition of root hair development. (Bio)Ag NPs stimulated ROS production in roots and affected the metabolic profiles of all tissues. Roots accumulated sucrose, maltose, 1-kestose, phosphoric acid, and some amino acids (i.e., proline, aspartate/asparagine, hydroxyproline, and branched-chain amino acids). In coleoptile and endosperm, contrary to roots, the concentration of most metabolites decreased. Moreover, coleoptile accumulated galactose. Changes in the concentration of polar metabolites in seedlings revealed the affection of primary metabolism, disturbances in the mobilization of storage materials, and a translocation of sugars and amino acids from the endosperm to growing seedlings.     

Network Bending: Expressive Manipulation of Generative Models in Multiple Domains

This paper presents the network bending framework, a new approach for manipulating and interacting with deep generative models. We present a comprehensive set of deterministic transformations that can be inserted as distinct layers into the computational graph of a trained generative neural network and applied during inference. In addition, we present a novel algorithm for analysing the deep generative model and clustering features based on their spatial activation maps. This allows features to be grouped together based on spatial similarity in an unsupervised fashion. This results in the meaningful manipulation of sets of features that correspond to the generation of a broad array of semantically significant features of the generated results. We outline this framework, demonstrating our results on deep generative models for both image and audio domains. We show how it allows for the direct manipulation of semantically meaningful aspects of the generative process as well as allowing for a broad range of expressive outcomes.     

Development of Optogenetic Dual-Switch System for Rewiring Metabolic Flux for Polyhydroxybutyrate Production

Several strategies, including inducer addition and biosensor use, have been developed for dynamical regulation. However, the toxicity, cost, and inflexibility of existing strategies have created a demand for superior technology. In this study, we designed an optogenetic dual-switch system and applied it to increase polyhydroxybutyrate (PHB) production. First, an optimized chromatic acclimation sensor/regulator (RBS10–CcaS#10–CcaR) system (comprising an optimized ribosomal binding site (RBS), light sensory protein CcaS, and response regulator CcaR) was selected for a wide sensing range of approximately 10-fold between green-light activation and red-light repression. The RBS10–CcaS#10–CcaR system was combined with a blue light-activated YF1–FixJ–PhlF system (containing histidine kinase YF1, response regulator FixJ, and repressor PhlF) engineered with reduced crosstalk. Finally, the optogenetic dual-switch system was used to rewire the metabolic flux for PHB production by regulating the sequences and intervals of the citrate synthase gene (gltA) and PHB synthesis gene (phbCAB) expression. Consequently, the strain RBS34, which has high gltA expression and a time lag of 3 h, achieved the highest PHB content of 16.6 wt%, which was approximately 3-fold that of F34 (expressed at 0 h). The results indicate that the optogenetic dual-switch system was verified as a practical and convenient tool for increasing PHB production.     

Anisotropic phononic crystal structure with low-frequency bandgap and heat flux manipulation

This study presents a two-dimensional phononic crystal with heat flux manipulation and wide bandgaps of out-of-plane modes within the low-frequency range. The anisotropic matrix made of spiral-multilayered materials with different thermal conductivities, and the coating layer inserted with metal are designed for heat flux manipulation. Rubber-coated metal cylinders are periodically embedded in the anisotropic matrix to obtain the low-frequency bandgaps of out-of-plane modes. Numerical simulation is carried out to validate the heat and elastic characteristics of the spiral-multilayered anisotropic structure and reveal the effects of the laying angle and temperature on the bandgaps. Subsequently, a spiral-multilayered plate with periodic structures is studied, which shows an obvious vibration attenuation in the frequency ranges of the bandgaps and a deflected heat flux from the initial propagation direction. In the experimental investigation, the multi-phase spiral-multilayered anisotropic plate is simplified to a single-phase anisotropic plate made of aluminum. The characteristics of this type of anisotropic phononic crystal structure may pave the way for the design of a new kind of thermo-acoustic metamaterial serving in combined thermal and acoustic environments.     

Noncontact atomic force microscopy: Bond imaging and beyond

It was a long-cherished dream for chemists to take a direct look at chemical bonding, a fundamental component of chemistry. This dream was finally accomplished by the state-of-the-art noncontact atomic force microscopy (NC-AFM) equipped with qPlus force sensors and carbon monoxide (CO) functionalized tips. The resolved interconnectivity between atoms and molecules in NC-AFM frequency shift images is interpreted as chemical bonding, providing essential knowledge of the bond length, bond angle and even bond order. The featured contrast of different chemical bonds can serve as fingerprints for further interpretation of chemical structures toward unknown species synthesized on surfaces. This breakthrough enriches characterization tools for surface science and brings our understanding of on-surface reactions to a new level. Beyond bond imaging, the application of NC-AFM has been extended to quantifying interatomic interactions, identifying three-dimensional nanostructures, manipulating molecules and reactions, as well as determining molecular electronic characteristics. Moreover, some recent efforts address the improvement of the usability and versatility of the bond-resolved NC-AFM technique, including high-resolution molecular investigation on bulk insulators, application-specific tip modification, stable bond imaging above liquid helium temperature and autonomous experimentation implemented by artificial intelligence.     

Non-Lithography Hydrodynamic Printing of Micro/Nanostructures on Curved Surfaces

A key issue of micro/nano devices is how to integrate micro/nanostructures with specified chemical components onto various curved surfaces. Hydrodynamic printing of micro/nanostructures on three-dimensional curved surfaces is achieved with a strategy that combines template-induced hydrodynamic printing and self-assembly of nanoparticles (NPs). Non-lithography flexible wall-shaped templates are replicated with microscale features by dicing a trench-shaped silicon wafer. Arising from the capillary pumped function between the template and curved substrates, NPs in the colloidal suspension self-assemble into close-packed micro/nanostructures without a gravity effect. Theoretical analysis with the lattice Boltzmann model reveals the fundamental principles of the hydrodynamic assembly process. Spiral linear structures achieved by two kinds of fluorescent NPs show non-interfering photoluminescence properties, while the waveguide and photoluminescence are confirmed in 3D curved space. The printed multiconstituent micro/nanostructures with single-NP resolution may serve as a general platform for optoelectronics beyond flat surfaces.     

促红细胞生成素B螺旋表面肽的代谢稳定性结构修饰：设计、合成及其提高的肾保护作用

肾脏缺血缺氧以及再灌注过程都将导致肾小管上皮细胞凋亡,使肾功能严重受损.肾脏的缺血再灌注损伤是移植肾功能延迟恢复的主要原因并能诱导急慢性排斥,影响肾存活率.近年来发现,衍生于促红细胞生成素（EPO）的B螺旋亚基亲水表面序列的肽链（HBSP）,对肾脏缺血再灌注损伤具有显著的保护作用,但其在体内极短的半衰期（约2min）极大地限制了它的临床应用.因此,本研究采用构象约束、全D-构型氨基酸替换和N-端封闭策略,设计了3种类型的EPOB螺旋表面肽衍生物,旨在提高其代谢稳定性环肽的设计采用了对氧化还原稳定的硫醚键和相对刚性的亚砜键两种环合方式.在多肽的合成上,采用微波辅助多肽自动合成和手工合成两种模式有机结合;优化了硫醚环合工艺,应用微波加热进行硫醚环肽的合成,大大提高了产率和效率;利用圆二色（CD）谱确定了亚砜环肽的相对构型.活性实验表明,相对于线性母肽HBSP,本文设计合成的代谢稳定衍生肽对大/小鼠肾脏缺血再灌注损伤均有显著提高的保护作用,且硫醚和R-构型亚砜环肽的肾脏保护活性强于S-构型亚砜环肽.而且,环化确实提高了功能肽的血浆稳定性.因此,本文合成的硫醚环肽一周一次注射剂量等效于线性肽HBSP一日三次剂量对小鼠肾损伤的保护作用.     

Biotransformation and metabolic profile of anemoside B4 with rat small and large intestine microflora by ultra‐performance liquid chromatography–quadrupole time‐of‐flight tandem mass spectrometry

Pulsatilla chinensis (Bunge) Regel is commonly used in Asia, and anemoside B4 (AB4) is its major saponin, with diverse pharmaceutical effects. Previous studies showed that intestinal flora plays an important role in the metabolism of herbs administered orally. In this study, the metabolic profile of AB4 with microflora in rat small and large intestines in vitro was investigated. Gut microflora was collected from different intestinal segments and anaerobically incubated with AB4 at 37°C for 24, 48, 72 and 96 h, respectively. A total of 10 metabolites were detected and identified by ultra‐ performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry, involving the products of oxygenation and deglycosylation reactions. Gut microflora in the large intestine generated more comprehensive metabolic pathways, which appears to be attributable to the wider range of bacterial types and numbers of bacteria. Human cancer cell lines SMMC‐7721, Hela and MCF‐7 were treated with metabolite pools by MTT assay, together with M6 as the greatest deglycosylation product. As a result, M6 exhibited a reduction in cell viability of SMMC‐7721 with an IC₅₀ value of 22.28 ± 1.26 μg/mL. The present study provided scientific evidence for AB4 metabolism in small and large intestines, which is helpful to reveal the active forms of AB4 in vivo .     

Inter-particle interaction induced by broadband radiation

The recent fabrication of optical traps using super-continuum light impresses the need for a theory of inter-particle interactions under such conditions. Development of the theory provides a basis for calculation of the observations expected under experimental conditions. An expression for the inter-particle potential energy induced by continuum states of light is first derived using quantum electrodynamics. This energy expression is cast as a function of the spectral irradiance of the light, and the electric susceptibility of the interacting particles. Specific results are derived for light with a Lorentzian spectrum. It is shown that by filtering part of the spectrum, it is possible to exert control over the length of linear particle chains organized along the Poynting vector. The results exhibit scope for the optical fabrication of moldable structures using broadband light.