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11.
A piezoelectric nucleic acid sensor was constructed ofr detection of tumor necrosis factor gene.Two methods were employed for immobilization of nucleic acid probe on gold electrode of piezoelectric crystal.The results show that polyethyleneimine adbesion and glutaraldehyde cross-linking method has higher sensitivity,stability and selectivity than protein A method.The solid-phase nucleic acid hybridization of oligo unclecotides and tumor necrosis factor target gene sequence were monitorde using this sensor.Tumor necrosis factor gene sequence(580bp) was detected by this nucleic acid sensor for the first time. 相似文献
12.
Synthesis of porphyrin nitrogen mustards with potential anti-tumor activities in chemotheraphy and photodynamic therapy 总被引:3,自引:0,他引:3
2,7,12,18-Tetramethyl-13,17-di[3'-N,N'-di(2"-chloroethyl)aminopropyl]porphin and it's 3,8-di(1'-alkyloxyethyl)-analogous or porphyrin-nitrogen mustards were synthesized for the first time Their structures were determined by spectroscopics and elemental analyses.Most of the compounds possess both the chemotherapeutic and photodynamic effects on tumor and deserve further investigation. 相似文献
13.
Tetsuro Kato Ryosuke Nemoto Hisashi Mori Ryoetsu Abe Katsuo Unno Akio Goto Hideyuki Murota Masaoki Harada Motofumi Homma 《Applied biochemistry and biotechnology》1984,10(1-3):199-211
To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing
an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes
of FM-MMC-mc were made: one with the drug as the core and zinc ferrite on its capsular surface (outer type); the other with
both the drug and zinc ferrite as the core (inner type). Both preparations provided a sustained-release property and a sensitive
response to conventional magnetic force, although certain differences in the release rate of drug, magnetic responsiveness,
and particle size were found between the two dosage forms. Animal studies showed that the magnetic microcapsules could be
magnetically controlled in the artery and urinary bladder. VX2 tumors in the rabbit hind limb and urinary bladder were successfully
treated with magnetic control of FM-MMC-mc. Pharmacokinetic study revealed that the targeting of the microcapsules markedly
enhanced the drug absorption into the surrounding tissues for a prolonged period of time. The results indicate the feasibility
and effectiveness of the magnetic microcapsules as a targeted drug delivery system. 相似文献
14.
《Arabian Journal of Chemistry》2022,15(7):103933
In this study, a fingerprint-activity relationship modeling between chemical fingerprints and antirheumatic activity was established, and multivariate statistical analysis was used to evaluate the quality of Taxilli Herba (TH) from different hosts. Characteristic fingerprints of 20 batches of TH samples were generated by high-performance liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (HPLC-Triple TOF-MS/MS), and the similarity analysis was calculated based on thirteen common characteristic peaks by hierarchical clustering analysis (HCA). Subsequently, nine efficacy markers were discovered by combining fingerprints and antirheumatic activity through grey correlation analysis (GCA) and bivariate correlation analysis (BCA). Meanwhile, the content of 5 constituents in 9 markers was determined by high-performance liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (HPLC-QTRAP-MS/MS). The comprehensive quality of TH was assessed using multivariate statistical analysis, including principal components analysis (PCA) and technique for order preference by similarity to ideal solution (TOPSIS). The results showed that a high dose of TH extract could markedly ameliorate arthritis damage compared to other doses, with flavonoids playing an important role in the antirheumatic activity. The comprehensive quality of samples from Morus alba L. (SS) was superior to those from Liquidambar formosana Hance (FXS). The present study will demonstrate the markers associated with efficacy, and provide an applicable strategy for more comprehensive quality control and evaluation of TH. 相似文献
15.
J. Monaselidze Ya. Kalandadze D. Khachidze 《Journal of Thermal Analysis and Calorimetry》1996,46(2):431-440
The influence of mitoxantron (M) and benz(a)pyrene (BP) on chromatin DNA in composition of spleen and liver tissues and cells of BALB/c mice were studied using a high-sensitive differential scanning microcalorimeter. It was established that BP can cause a) the specific breaks in inactive chromatin DNA chain and unfolding of the whole domain (a loop of chromatin) which should lead to uncontrolled genome activation; b) the breaks in the DNA double-helix creating short duplexes.M at low doses, interacting with naked linker DNA of tumor restores the chromatin structure, at high doses or at repeated injections,M causes the disturbance of chromatin structure. 相似文献
16.
张易青 《青海师范大学学报(自然科学版)》2003,(3):80-81
2000—2002年进行颈部肿块细针吸取细胞学检查共600例,有病理组织学证实138例,其中恶性病变66例。淋巴结、甲状腺各占60.10%、23.50%。细胞学阳性158例,占26.33%,可疑恶性32例,占5.33%。和术后病理组织学诊断对照。恶性病变符合率80.30%,良性病变符合率为93.06%,总符合率为86.96%。敏感率(阳性 可疑)淋巴结、甲状腺分别为96.29%和90.00%。本文对假阳性、假阴性病例进行了分析。 相似文献
17.
on the human tumor cell growth MA Yewei ZHOU Xiaoshan QIAN Xinlai ZHAO Qingzheng YANG Jun GAO Xin LI Yanchun LIU Yuying & WANG Zheng . Cancer Institute Peking Union Medical College Chinese Acad-emy of Medical Sciences Beijing China . Beijing Institute of Blood Transfusion Medicine Beijing China 《科学通报(英文版)》2003,(7)
The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha… 相似文献
18.
张荣生 《大理学院学报:综合版》2006,5(4):16-17
目的:探讨胸腔注入顺铂(DDP)联合白细胞介素-2(IL-2)治疗恶性胸腔积液疗效。方法:58例患者均病理确诊为恶性胸腔积液,随机分为二组,治疗组(32例)在胸腔内注入DDP,白细胞介素-2,对照组(26例)在胸腔内注A.DDP,两组均在1周及2周后各重复注药1次,共3次,观察疗效,毒副反应及生活质量。结果:治疗组总有效率为84.4%,对照组57.7%,差异有显著性(P〈0.015)。治疗组Kamofsky评分〉70分者较对照组有显著提高(P〈0.05),两组在毒性反应上无显著性差异(P〉0.05)。结论:顺铂白介素-2胸腔灌注能有效控制恶性胸腔积液,毒副作用少,联合疗效肯定。 相似文献
19.
槲皮素抗癌作用的分子机制 总被引:7,自引:0,他引:7
槲皮素是已知最强的抗癌剂之一。从分子水平的角度阐述了其抗癌作用机制的研究进展。其中包括结合雌激素受体,下调变异的P53蛋白,阻滞细胞周期,抑制酪氨酸激酶,促进凋亡,阻遏热休克蛋白和抑制Ras的表达等等。随着其分子机制的深入研究,可以为临床治疗提供更好的理论依据。 相似文献
20.
利用频率为2.0 MHz,强度为5.0 W/cm2的超声激活血卟啉对在体小鼠艾氏腹水瘤细胞 进行了抑癌作用研究.通过对一次和多次处理的肿瘤体积和重量变化趋势的研究,发现声动力疗法 对艾氏腹水瘤细胞增殖有显著抑制作用,且多次处理比一次处理效果更为明显. 相似文献