Use of a pentachlorophenol degrading bacterium to bioremediate highly contaminated soil

ASphingomonas species that mineralizes high concentrations of pentachlorophenol (PCP) was isolated from a PCP-contaminated EPA Superfund site. This bacterium, identified asSphingomonas sp. strain RA2, is able to degrade PCP at concentrations of up to 300 μg/mL in liquid culture. This organism was tested for its ability to degrade high concentrations of PCP in a soil that did not contain organisms capable of degrading high concentrations of PCP. When inoculated into contaminated soil,Sphingomonas sp. RA2 mineralized PCP at concentrations of 300, 600, 900, and 1200 μg PCP/g of soil, but was unable to mineralize 1500 μg PCP/g of soil. Only very minimal loss of PCP was seen in uninoculated soils. The results of this study demonstrate thatSphingomonas sp. RA2 may be a useful organism for remediation of sites contaminated with high concentrations of PCP.     

离体条件下影响芦荟增殖的若干因素

从接种方式、茎基部直径、培养方式、糖浓度、无机盐浓度和接种芽的状态等方面研究了影响离体条件下芦荟增殖的因素。结果表明：劈接是目前最好的方式；芦荟茎基部直径对其再生率有影响，0．5cm最好；增殖培养基中蔗糖浓度3％和1／8MS无机盐浓度最好。     

浅谈金针菇的栽培技术

金针菇是别具一格的食用菌,既有蘑菇的鲜美味,又有银耳嫩滑的胶质,还有金针菜清脆的甘甜味。发展金针菇栽培具有较大的经济效益。本文对每一过程中所必需的技术要点和注意事项进行了初步分析。     

接种量对泔脚发酵产氢余物甲烷化的强化研究

 以城市生活垃圾厌氧消化污泥为接种物,考察了15％、30％、45％、60％的接种量对泔脚发酵产氢余物中温（36℃）批式厌氧消化的影响。结果表明：泔脚发酵产氢余物的产沼能力随着接种量的增大而增强。结合Gompertz模型拟合结果及理论分析,60%的接种量为泔脚发酵产氢余物沼气化的最佳接种量,其沼气化的延迟时间λ、产甲烷率、生物气中甲烷的最高体积含量分别为：4.37d,388.72mL/g,80.4％。消化结束后,除产沼过程失败的15％接种量以外,厌氧消化余物的pH值在 6.70～7.00之间,这为沼气中甲烷的高体积含量（高于常规厌氧消化的最高甲烷体积含量75%）提供了证据。     

B和B+Ti孕育对β相铜基合金的后作用

本文给出了β相Cu基合金在固溶化处理和轧制过程中,B和B+Ti孕育对试样晶粒生长的后作用的研究结果.根据相应试样的断裂性能、断口形貌和含B析出物的分析,探讨了它们的后作用机理.最后,提出了获得优质Cu ZnAl形状记忆合金材料的最佳工艺.     

葡萄幼胚培养技术的研究(Ⅰ)——激素对胚培养的影响及培养基的筛选

选用巨峰、里扎马特和无核白葡萄的幼胚接种在附加了GA3、IAA、6-BA等的不同激素水平的MS培养基上进行培养,结果表明花后60d的巨峰和花后50d的无核白的幼胚在附加GA30.5mg.L-1、IAA2.0mg.L-1、6-BA0.5mg.L-1的培养基上分别得到了44.0±8.9%和96.0±8.9%的发育率与31.9±6.4%和37.5%的成苗率;花后60d的里扎马特幼胚在附加GA30.5mg.L-1、IAA0.5mg.L-1、6-BA0.5mg.L-1的培养基上得到了76.0±8.9%的发育率和63.5±5.5%的成苗率.筛选出了适合不同品种的最佳胚培养、萌发以及成苗培养基,为葡萄新品种的选育提供了新的方法与技术依据.     

Preclinical Testing of Radiopharmaceuticals for the Detection and Characterization of Osteomyelitis: Experiences from a Porcine Model

The development of new and better radioactive tracers capable of detecting and characterizing osteomyelitis is an ongoing process, mainly because available tracers lack selectivity towards osteomyelitis. An integrated part of developing new tracers is the performance of in vivo tests using appropriate animal models. The available animal models for osteomyelitis are also far from ideal. Therefore, developing improved animal osteomyelitis models is as important as developing new radioactive tracers. We recently published a review on radioactive tracers. In this review, we only present and discuss osteomyelitis models. Three ethical aspects (3R) are essential when exposing experimental animals to infections. Thus, we should perform experiments in vitro rather than in vivo (Replacement), use as few animals as possible (Reduction), and impose as little pain on the animal as possible (Refinement). The gain for humans should by far exceed the disadvantages for the individual experimental animal. To this end, the translational value of animal experiments is crucial. We therefore need a robust and well-characterized animal model to evaluate new osteomyelitis tracers to be sure that unpredicted variation in the animal model does not lead to a misinterpretation of the tracer behavior. In this review, we focus on how the development of radioactive tracers relies heavily on the selection of a reliable animal model, and we base the discussions on our own experience with a porcine model.     

发酵法生产番茄红素培养方法的改进及优化

研究了应用三孢布拉氏霉菌生产番茄红素的发酵工艺。针对菌种生长能力的降低,首次采用了孢子悬液直接接种的方式,并对菌种孢子的生长条件进行了优化。另外,提出了直接发酵培养的一级培养工艺,该工艺稳定、简便,不仅提高了番茄红素的产量,而且缩短了发酵培养时间。