Construction of ice nucleation active <Emphasis Type="Italic">Enterobacter cloacae</Emphasis> for control of insect pests

Ice nucleation active(INA)bacteria are the most potent heterogeneous ice nuclei in nature,which have become an important biological resource for diverse applications.Many researches have proved that INA bacteria can raise the supercooling points(SCPs)of insect pests,then reduce their cold hardiness.However,INA bacteria‘s inefficient colonization on the surface or in the guts of insects,and the high incidence of frost injury induced by their release hampered the application of INA bacteria in controlling insect pests in agricultural fields.In this study,we constructed a recombinant plasmid mob-Tn5-iceA with the ability of broad-host-range conjugal mobilization and integration of the ina gene of icaA into chromosomal DNA of many gram-negative bacteria by Tn5 transposition.In addition,Ent.cloacae strains stably carrying iceA and expressing high ice nucleation activity(INA),even in the absence of antibiotic pressure,were constructed through conjugal mobilization and Tn5 transposition.Ent,cloacae strains have benn reported to be able to efficently colonize in the guts of insects,but have weak plant epiphytic ability.Therefore,these transgenic Ent.Cloacae may be promising candidates for control of insect pests in agricultural fields.     

核对称轴不同相对取向对熔合动力学的影响

计算了核对称轴不同相对取向时的熔合位垒.基于双核模型观念，考虑了熔合与准裂变的竞争，通过数值法求解主方程，计算了⁷⁶Ge+²⁰⁸Pb，⁴⁸Ca+²⁴⁴Pu核对称轴不同相对取向对熔合概率的影响，探索了最有利于超重元素合成的弹靶相对取向.取向不同时，对熔合反应的影响较大，计算结果表明弹靶碰撞为腰对腰时，更有利于发生熔合反应. 关键词： 超重元素 熔合概率 变形核 方向角度     

抗冰导管架平台结构动力安全评估

基于现场冰激振动监测资料，论述了抗冰导管架平台进行动力失效评估的必要性，分析了在动冰荷载作用下抗冰导管架平台的动力失效模式，指出动力失效模式主要包括振动失效模式和疲劳失效模式，并介绍了基于现场实测冰力数据建立的直立结构冰力谱的计算方法．作为应用实例，利用ANSYS软件建立了Jz9—3GCP平台的有限元模型，分析了其结构特性，并对其进行动力结构安全评估． 此方法对于抗冰平台的设计和安全评估具有一定的借鉴意义．     

Recent temperature increase recorded in an ice core in the source region of Yangtze River

Interests on climate change in the source region of Yangtze River have been raised since it is a region with the greatest warming over the Tibetan Plateau (TP). A 70-year history of precipitation δ~(18)O has been recovered using an ice core record retrieved in a plat portion of the firn area in the Guoqu Glacier (33°34′37.8″N, 91°10′35.3″E, 5720 m a.s.l.), Mt. Geladaindong (the source region of Yangtze River), in November, 2005. By using a significant positive relationship between ice core δ~(18)O record and summer air temperature (July to September) from the nearby meteorological stations, a history of summer air temperature has been reconstructed for the last 70 years. Summer temperature was relatively low in 1940s and high in 1950s to the middle of 1960s. The lowest temperature occurred in the middle of 1970s. Temperature was low in 1980s and dramatically increased since 1990s, keeping the trend to the begin-ning of the 21st century. The warming rate recorded in the ice core with 0.5℃/10 a since 1970s is much higher that that in the central TP and the Northern Hemisphere (NH), and it becomes 1.1℃/10 a since 1990s which is also higher than these from the central TP and the NH, reflecting an accelerated warm-ing and a more sensitive response to global warming in the high elevation region.     

英语语调调核位置探析

从英语语调结构和信息结构理论出发,揭示了英语调核位置的选择主要是根据整个语调组的信息中心--主要信息或新信息在哪里而确定的,并进一步阐述了影响调核位置的因素:语言的上下文关系、讲话时的情景、心理因素(包括联想和习惯因素,以及情感状态).     

Excitatory effect of histamine on neuronal activity of rat cerebellar fastigial nucleus Jn vitro

The cerebellar fastigial nucleus （FN） holds an important role in motor control and body balance. Previous studies have revealed that the nucleus is innervated by direct hypothalamocerebellar hletaminergic fibers. However, the functional role of histaminergic projection in cerebellar FN has never been established. In this study, we investigated the effect of histamine on neuronal firing of cerebellar FN by using slice preparations. Sixty-five FN cells were recorded from 47 cerebellar slices, and a vast majority of the cells responded to histamine stimulation with an excitatory response （58/65, 89.2%）. Perfusing slices with low-Ca^2＋/high-Mg^2＋ medium did not block the histamine-induced excitation （n=10）, supporting a direct postsynaptic action of histamine on the cells. Furthermore, the excitatory effect of histamine on FN neurons was not blocked by selective histamine H1 receptor antagonist triprolidine （n=15） or chlorpheniramine （n=10）, but was effectively suppressed by ranitidine （n=15）, a highly selective histamine H2 receptor antagonist. On the other hand, highly selective histamine H2 receptor agonist dimaprit （n=20） instead of histamine HI receptor agonist 2-pyridylethylamine （n=16） mimicked the excitatory effect of histamine on FN neurons. The dimaprit-induced FN neuronal excitation was effectively antagonized by selective histamine H2 receptor antagonist ranitidine （n=13） but not influenced by selective histamine H1 receptor antagonist triprolidine （n=15）. These results demonstrate that histamine excites cerebellar FN cells via the histamine H2 receptor mechanism and suggest that the hypothalamocerebellar histaminergic fibers may modulate cerebellar FN-mediated sensorimotor integration through their excitatory innervations on FN neurons.     

基于产品概念设计理论的快速冰酒器三维结构的设计

本文主要包括的是产品概念设计的要求和作用的分析和理论知识结合实际的设计实例。快速冰酒器作为产品概念设计的一个实例，对其功能，造型，结构，材料，色彩和各个零部件的连接和装配关系等要素进行设计。采用pro／E软件建模概念产品快速冰酒器的零件结构图，部分装配图和最终效果图。还包括有关的文字解释和总结等内容。     

超重核区的双幻性质研究

应用V-S公式、连续介质模型和Strutinsky壳修正能量理论计算结果，对SHEs区的核素进行α衰变能及寿命、基态形状及其变化和壳能级结构进行了研究。并对各种理论模型计算结果进行比较分析，认为298114最有可能成为超重核中唯一的球形双幻核。     

偶坐标S=18～6核素分布的特点

在正方形核素图中，坐标偶常数Ｓ＝１８－６的核素分布显示出系统的规律性，这七个偶Ｓ常数列的连续核表现出坐标Ｓ＝１８有７核，坐标Ｓ＝４与１２各有５核，坐标Ｓ＝１０至６的四列都是三核，呈现出以１，２，４组相同的递变特点。它们的范围下界是坐标Ｈ＝０，偶偶核以坐标（１２，４）为中心联系，其首中终坐标（６，２）（１２，４）（１８，６）以（６，２）的倍数联系。     

Dynamics of Ice Surfaces

The dynamics of faceted and partially faceted single crystal and polycrystalline ice are reviewed with an emphasis on the manner in which microscopic effects produce macroscopic shapes. Our understanding of the former is rooted in the basic kinetics common to all materials and our pursuit of the latter is largely motivated by the striking patterns exhibited in the natural environment when water changes phase to become ice.