Characteristics and production of thermostable α-amylase

Thermostable α-amylases have application in a variety of industrial processes and enzymes from a substantial number of thermophilic bacteria and fungi have been screened and characterized to varying degrees. The characteristics of these enzymes are summarized in this review. The genetics of α-amylase production inBacillus subtilis is reviewed and classical and recombinant DNA approaches to increasing α-amylase production are discussed.     

Xylanolytic enzyme production by anAspergillus niger isolate

Production of xylanolytic enzymes by anAspergillus niger CCMI 850 isolate was investigated in batch cultures. The effect of the composition of a fermentation medium that did not include chemical inducers, on β-xylanase, β-xylosidase, α-l-arabinofuranosidase, and total cellulase activity was studied. With 4% xylan as the carbon source, about 65 U/mL of β-xylanase was obtained, whereas the total cellulase activity was undetectable, under the specified conditions. This β-xylanase activity represents the highest reported for a wild-type strain ofA. niger. The effect of pH and temperature on the activity of β-xylanase was studied. Partial characterization of the β-xylanase showed that with insoluble birchwood as substrate theK _m andV _max were 0.3 mM and 19 μmol/min, respectively. Aspects of using the crude β-xylanase preparation for applications in the pulp and paper industry were discussed.     

分解水制氢的KNO3—I2循环之研究：Ⅲ.代替电解反应的一个子循环

本文提出了一个硫酸中间盐子循环用以代替分解水制氢的KNO_3-I_2混合循环中的电解反应,从而使原混合循环变为一个纯热化学循环。     

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

The development of express method for detection of endocrine-disrupting chemicals (EDC) such as alkylphenols is required for ecological monitoring. Several attempts have been made to produce antibodies against 4-nonylphenol (NP) in recent years. This work describes the production of new antibodies against NP and also summarizes the characterization of antibodies obtained earlier. Three approaches used to produce alkylphenol-specific antibodies are compared; these are based on: 1. omega-(4-hydroxyphenyl)nonanoic or omega-(4-hydroxyphenyl)heptanoic acid NP derivatives designed to mimic the linear NP isomer; 2. 4-aminophenol, which potentially mimics various substituted phenolic compounds with different side-chain structures at position 4 of the benzene ring; and 3. a mixture of branched NP isomers, conjugated to the carrier protein via a benzene ring by the Mannich reaction, and expected to be the closest mimic of NP structure by preserving its natural alkyl moiety.Fluorescence polarization immunoassays based on different combinations of antibody and labeled antigen for screening detection of NP were developed and structural aspects of assay sensitivity and specificity were investigated. The assays based on the antisera raised against omega-(4-hydroxyphenyl)nonanoic acid and NP conjugate via Mannich reaction are capable of express detection of NP with detection limit of 7 microg mL(-1 )and assay dynamic range of 18-300 microg mL(-1).     

Superoxes,high temperature universal phases in (GC)2

Most of the common classes of organic compounds chromatograph normally on Superoxes. There is no tailing or adverse effect from excessively different activity different activity coefficients. Superoxes are therefore universal phases for gas chromatography. This is also expressed by a wide useful temperature range from ～50° to ～300°. High MW Superox-4 has a MAOT about 20° higher than the lower MW Superox-0.1. Several applications illustrating the versatility of Superox phases in (GC)² are presented.     

煤炭企业固定工的地位及现存问题的改进

本文对当前煤炭企业用工制度改革存在的问题、改进途径和各种形式用工的比例进行了分析,并指出为适应煤矿安全生产及机械化发展的需要,保持固定工的用工比例,克服“铁饭碗在手”等隋性意识,充分发挥他们的骨干作用是问题的关键。为此,本文提出了改变工资结构、劳动结构等相应的改革措施。     

基于水光电力系统时序生产模拟模型的水光优化配比研究

基于考虑系统负荷、新能源发电出力等时序变化特性的时序生产模拟法,建立了水光电力系统时序生产模拟模型;从系统结构性、经济性、灵活性与可靠性方面综合确定最佳水光容量配比,并通过金沙江上游川藏段水光系统验证了模型的合理性。金沙江上游川藏段光伏电站与水电站出力具有较好的年内、日内互补特性;对于2030年水平年,考虑枯期水电留存,913.6万kW水电配套400万kW光伏的方案综合性能更优,其新增非水可再生电量约60亿kW·h。     

Positivity of entropy production in nonequilibrium statistical mechanics

We analyze different mechanisms of entropy production in statistical mechanics, and propose formulas for the entropy production ratee() in a state . When is steady state describing the long term behavior of a system we show thate()0, and sometimes we can provee()>0.     

批量制造业模糊交货期准时化生产计划方法

通过建立批量制造业模糊交货期下的准时化生产计划模型,将隶属度函数引入到生产计划问题中,从而拓宽了准时化生产计划问题的研究范围,并为实际生产计划的编制提供了一种有效途径。