碱提芥菜多糖alp-2的分离纯化及理化性质分析

经水反复浸提后的包心芥菜残渣经稀碱浸提,浓缩,乙醇沉淀,透析,DEAE-Sepharose-F F色谱分离纯化得到一种白色粉末状多糖ALP-2。经醋酸纤维薄膜电泳和HPLC分析证明,ALP-2为纯品。ALP-2不含蛋白质、核酸,为非淀粉类均一多糖,其相对分子量为459873。ALP-2完全酸水解后HPLC分析确定糖基组成为鼠李糖、阿拉伯糖、葡萄糖、半乳糖和另外一个待确定的组分。     

茶叶中氟含量及其影响因素

通过不同茶园中茶叶的氟含量、土壤主要理化性质和土壤水溶性氟含量的调查 ,探讨影响茶叶中氟含量的几种主要因素 .结果表明 ,茶叶氟含量随叶龄的增加而增加 ;与土壤理化性质的关系并不显著 ,与土壤水溶性氟呈显著相关关系 .此外 ,土壤水溶性氟与土壤理化性质呈显著相关关系 .     

废次烟叶中茄尼醇的超声提取及HPLC分析测定

废次烟叶中茄尼醇的超声提取及HPLC分析测定;超声提取;高效液相色谱;废次烟叶;茄尼醇     

基于三通道注意力网络的番茄叶部病害识别

对番茄病害进行识别,近年来一直是植物病害预防的研究热点。由于受到复杂背景干扰,番茄叶部病害识别准确率不高,针对这一问题,提出一种基于三通道注意力机制网络的番茄叶部病害识别方法。该网络基于ResNeXt50残差网络,将注意力模块嵌入至残差网络的ResNeXt模块中可以并行提取目标的通道特征和空间特征,获取有效的语义信息。训练阶段通过设计双损失函数和数据增强进一步提升分类准确度,并通过迁移学习网络预训练参数的方式提高网络训练效率。实验结果表明,使用双损失函数和数据增强后,基于三通道注意力网络的番茄病害识别算法在测试集上的平均识别准确率达98.4%,相比于传统机器学习方法和其他神经网络方法的准确率更高,检测速度满足实时性,Kappa系数为0.96,满足叶部病害识别的高精度要求。该方法能够有效地对10种番茄叶部病害进行识别,为植物病害识别提供了一种新的思路。     

复杂载荷下硬涂层对整体叶盘的减振特性

为了研究硬涂层对航空发动机整体叶盘在复杂载荷下的振动影响,通过有限元分析的方法对比研究了其振动特性。首先,建立整体叶盘三维模型,导入ANSYS Workbench软件中,并在叶片的侧面添加NiCoCrAIY+YSZ硬涂层;其次,使用一维插值的方法对航空发动机整体叶盘进行稳态温度场设置,采用Fluent模块对整体叶盘气动载荷进行添加,运用预应力模态分析法研究在离心载荷、温度载荷和气动载荷作用下,硬涂层对整体叶盘非线性振动特性的影响;最后,绘制坎贝尔图,分析在不同转速下硬涂层整体叶盘的共振情况,采用谐响应分析研究其振动位移响应。结果表明：整体叶盘叶尖处更容易受到振动的影响;涂敷硬涂层可以降低整体叶盘的固有频率、响应峰值,且能减少共振点数量。可见硬涂层对整体叶盘减振具有积极影响。     

改进的主动形状模型在植物叶形分类上的应用

本文在继承主动形状模型以地标点序列表示对象形状轮廓,通过归一化序列间的统计关系来研究形状间的相似性和差异性,以及用识别率来体现识别效果这三个基本特征的同时,针对植物叶片识别的特点,对模型进行了改进：增加了独立参数和粗分类步骤,使叶片识别免受非形状分类因素的干扰;在归一化进程中分别应用覆盖面积半径和生物学参照点进行伸缩变换和旋转变换,使归一化更符合生物学特征;在分类时通过设置相似度阈值,强化了同类叶片的标准,从而减少了一些非同类的误归入事件的发生;最后设计了三组实验来研究各影响因素独立变化时对识别率的影响,同时引入了误差矩阵和相似度矩阵,分别来体现错分的事件的分布情况并揭示了产生错分的原因。经过这些改进,本文实现植物叶片图像的批量识别和分类,提供了一种查询错分分布的方法并指出提高识别率的研究方向。     

Antifungal Evaluation and Molecular Docking Studies of Olea europaea Leaf Extract,Thymus vulgaris and Boswellia carteri Essential Oil as Prospective Fungal Inhibitor Candidates

Background: The present study investigated the antifungal activity and mode of action of four Olea europaea leaf extracts, Thymus vulgaris essential oil (EO), and Boswellia carteri EO against Fusarium oxysporum. Methods: Fusarium oxysporum lactucae was detected with the internal transcribed spacer (ITS) region. The chemical compositions of chloroform and dichloromethane extracts of O. europaea leaves and T. vulgaris EO were analyzed using GC-MS analysis. In addition, a molecular docking analysis was used to identify the expected ligands of these extracts against eleven F. oxysporum proteins. Results: The nucleotide sequence of the F. oxysporum lactucae isolate was deposited in GenBank with Accession No. {"type":"entrez-nucleotide","attrs":{"text":"MT249304.1","term_id":"1824655600","term_text":"MT249304.1"}}MT249304.1. The T. vulgaris EO, chloroform, dichloromethane and ethanol efficiently inhibited the growth at concentrations of 75.5 and 37.75 mg/mL, whereas ethyl acetate, and B. carteri EO did not exhibit antifungal activity. The GC-MS analysis revealed that the major and most vital compounds of the T. vulgaris EO, chloroform, and dichloromethane were thymol, carvacrol, tetratriacontane, and palmitic acid. Moreover, molecular modeling revealed the activity of these compounds against F. oxysporum. Conclusions: Chloroform, dichloromethane and ethanol, olive leaf extract, and T. vulgaris EO showed a strong effect against F. oxysporum. Consequently, this represents an appropriate natural source of biological compounds for use in healthcare. In addition, homology modeling and docking analysis are the best analyses for clarifying the mechanisms of antifungal activity.     

3种抗寒保护剂对大叶黄杨叶片抗冻性的影响

聚乙二醇、氯化钙及二甲基亚砜3种抗寒保护剂单一喷施处理大叶黄杨叶片后,可减少冻害胁迫过程中电解质的渗透率及MDA含量,并且POD活性较对照组有所提高,这表明3种抗寒保护荆均能不同程度增强大叶黄杨叶片抗冻性,其中Ca^2＋作用效果最佳,同时对上述3种抗冻鉴定指标比较后认为,电解质渗透率能更灵敏、客观地评价大叶黄杨叶片抗冻性。     

Potts Model on Maple Leaf Lattice with Pure Three-Site Interaction

We use Monte Carlo method to study three-state Potts model on maple leaf lattice with pure three-site interaction. The critical behavior of both ferromagnetic and antiferromagnetic cases is studied. Our results confirm that the critical behavior of the ferromagnetic model is independent of the lattice details and lies in the universality class of the three-state ferromagnetic Potts model. For the antiferromagnetic case the transition is of the first order. We have calculated the energy jump and critical temperature in this area. We find there is a tricritical point separating the first order and second order phases for this system.     

基于蒙特卡罗法的植物叶片可靠性分析

为探索植物叶片各变量参数及加载载荷与其可靠性之间密切关系,采集石栗新鲜植物叶片,通过三坐标测量仪获取植物叶片的坐标数据点云,在ANSYS中建立简化的叶片有限元模型,基于蒙特卡罗法对叶片模型施加随机风载求解计算.结果表明:假如模拟次数足够多,运用ANSYS 软件与蒙特卡罗相结合的随机有限元法分析植物叶片的可靠性是可行的.