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161.
M. J. Medina Hernández S. Sagrado Vives M. C. García Alvarez-Coque 《Mikrochimica acta》1992,108(3-6):293-298
Automation by flow injection analysis with Spectrophotometric detection of the determination of total amino acids and proteins witho-phthalaldehyde is not straightforward. The use of spectrophotometry, instead of spectrofluorimetry, and of N-acetyl-L-cysteine, instead of the conventional mercaptoethanol is advantageous because of the lower variability of absorptivities with respect to fluorescence yields, and the larger stability of the derivatives. Under adequate working conditions and with leucine as reference, the procedure can be used for the evaluation of total amino acids. A similar procedure is proposed for the analysis of proteins in a sample. Limits of detection are 1 × 10–5
M for amino acids, and 1 × 10–6
M for proteins, respectively. 相似文献
162.
The present short review deals with electroanalytical aspects of electrochemical response of ascorbic acid (Vitamin C) at conducting and electrogenerated polymer modified electrodes. Two main topics are considered: (i) electrocatalytic oxidation of ascorbate at conducting polymer modified electrodes, leading to electroanalytical techniques for ascorbate assay, and (ii) retardation of ascorbate penetration through a layer of electrogenerated polymers, leading to permselective coatings and their diverse uses, especially for biosensing devices. 相似文献
163.
为了评估基于snappyHexMesh方法生成的自动网格体系在二维柱体绕流大涡模拟中的适用性,比较了该自动网格体系与人工网格体系对于Re为3 900圆柱绕流和Re为22 000方柱绕流的数值模拟结果。通过设置合理的计算域以及数值格式,采用snappyHexMesh自动网格以及人工网格的算例都表现出良好的数值稳定性。将不同网格体系的数值模拟结果与物理试验结果进行对比,结果表明,采用snappyHexMesh网格可以提高数值求解效率;圆柱绕流对网格体系的变化比较敏感,不同密度的snappyHexMesh网格会显著影响圆柱气动力特征以及尾流区域的流场结果;snappyHexMesh网格体系可以准确预测方柱绕流,在方柱绕流大涡模拟中具有相较于圆柱绕流更好的适用性。 相似文献
164.
针对人口老龄化背景下的城轨换乘站客流组织问题,从影响因素分析出发,量化老年乘客生理特征对乘客行动力影响的外在表现,并运用社会力模型描述换乘站内行人运动。借助AnyLogic仿真软件,以重庆市某换乘站为实验对象构建地铁换乘站仿真模型。通过设计多场景仿真实验,结果发现老年乘客比例对站内客流速度、进站时间、换乘时间都有明显影响,尤其是在站内客流瓶颈处和高差变化处的影响更为明显。最后,对适老背景下城轨车站设计和运营提出针对性建议。 相似文献
165.
静脉系统是心血管系统的重要组成部分.脉搏波在血液流动中有着突出的重要性.本文主要研究静脉血流动力学模型基本波的相互作用.血流动力学模型是2×2严格双曲型方程组,其基本波包括疏散波和激波,属于血液流动中的脉搏波.基本波相互作用后血管截面面积和血流速度发生相应的变化. 相似文献
166.
Analytical solutions of the lattice Boltzmann BGK model 总被引:1,自引:0,他引:1
Analytical solutions of the two-dimensional triangular and square lattice Boltzmann BGK models have been obtained for the plane Poiseuille flow and the plane Couette flow. The analytical solutions are written in terms of the characteristic velocity of the flow, the single relaxation time , and the lattice spacing. The analytic solutions are the exact representation of these two flows without any approximation. Using the analytical solution, it is shown that in Poiseuille flow the bounce-back boundary condition introduces an error of first order in the lattice spacing. The boundary condition used by Kadanoffet al. in lattice gas automata to simulate Poiseuille flow is also considered for the triangular lattice Boltzmann BGK model. An analytical solution is obtained and used to show that the boundary condition introduces an error of second order in the lattice spacing. 相似文献
167.
泵引起的液压系统振动分析 总被引:2,自引:0,他引:2
永青 《北京科技大学学报》1995,17(4):350-354
通过液压系统产生振动和噪音的振源及振动量传递的分析,开发了测定泵的振源特性的新方法,-2压力/2系统”法,介绍了用阻抗求解管内压力脉动的方法及通用仿真程序包,实验表明,仿真结果与实测结果一致。 相似文献
168.
Dietmar Krämer Erwin Rosenberg Achim Krug Robert Kellner Wolfgang Hutter Werner Hampel 《Mikrochimica acta》1994,116(4):183-189
Chloride is determined indirectly by Spectrophotometric flow injection analysis. Two systems are compared, both based on the principle of ion exchange of easily detectable anions versus chloride from suitable mercury salts. The first method is based on the exchange of chloride with chloranilate which is detected at 332 nm or at 306 nm in neutral or in acidic medium respectively. In the second case, chloride reacts with Hg(SCN)2. The liberated thiocyanate forms a strongly coloured complex with Fe(III) in acidic solution with an absorption maximum at 460 nm. Both methods have a detection limit of about 5 mol Cl–/l (175 ng/ml). In the case of the thiocyanate method, the relative standard deviation is about 2% (7 measurements) in the range of 5 to 150 mol/l and decreases significantly to a value of approximately 0.2% at higher concentrations; for the chloranilate method it is 10% for lower and about 1% for higher concentrations respectively. 相似文献
169.
LetN = (G, T, c, a) be a network, whereG is an undirected graph,T is a distinguished subset of its vertices (calledterminals), and each edgee ofG has nonnegative integer-valuedcapacity c(e) andcost a(e). Theminimum cost maximum multi(commodity)flow problem (*) studied in this paper is to find ac-admissible multiflowf inG such that: (i)f is allowed to contain partial flows connecting any pairs of terminals, (ii) the total value off is as large as possible, and (iii) the total cost off is as small as possible, subject to (ii). This generalizes, on one hand, the undirected version of the classical minimum cost maximum flow problem (when |T| = 2), and, on the other hand, the problem of finding a maximum fractional packing ofT-paths (whena 0). Lovász and Cherkassky independently proved that the latter has a half-integral optimal solution.A pseudo-polynomial algorithm for solving (*) has been developed earlier and, as its consequence, the theorem on the existence of a half-integral optimal solution for (*) was obtained. In the present paper we give a direct, shorter, proof of this theorem. Then we prove the existence of a half-integral optimal solution for the dual problem. Finally, we show that half-integral optimal primal and dual solutions can be designed by a combinatorial strongly polynomial algorithm, provided that some optimal dual solution is known (the latter can be found, in strongly polynomial time, by use of a version of the ellipsoid method).This work was partially supported by Chaire municipale, Mairie de Grenoble, France. 相似文献
170.
H. Kunita 《Journal of Theoretical Probability》1994,7(2):279-308
We discuss the Cauchy problem of a certain stochastic parabolic partial differential equation arising in the nonlinear filtering theory, where the initial data and the nonhomogeneous noise term of the equation are given by Schwartz distributions. The generalized (distributional) solution is represented by a partial (conditional) generalized expectation ofT(t)°
0,t
–1
, whereT(t) is a stochastic process with values in distributions and
s,t
is a stochastic flow generated by a certain stochastic differential equation. The representation is used for getting estimates of the solution with respect to Sobolev norms.Further, by applying the partial Malliavin calculus of Kusuoka-Stroock, we show that any generalized solution is aC
-function under a condition similar to Hörmander's hypoellipticity condition. 相似文献