Formation of Glyco-Functionalized Platinum Complexes by Cross-Metathesis and Evaluation of Their Efficacy in Inhibition of Lung Tumor Cell Lines

Syntheses of carbohydrate-functionalized platinum complexes resembling presently employed cytostatics were performed. Mono-allylated sugar substrates obtained in two steps from glucose and galactose were connected with 2-allyl diethyl malonate by cross-metathesis. Following hydrogenation and acidic cleavage of the ester and alkylidene functionalities gave dicarboxylated glycoconjugates, which were transformed into their diammine platinum complexes. The antitumor activities of these platinum complexes were checked by sensitivity testing with 11 lung cancer cell lines. The novel glucose-platinum complex proved to be comparable to the drug carboplatin.     

Garcinielliptone G from Garcinia subelliptica Induces Apoptosis in Acute Leukemia Cells

Cytotoxicity and apoptosis-inducing properties of compounds isolated from Garcinia subelliptica leaves were investigated. The hexane-soluble portion of MeOH extracts of G. subelliptica leaves that showed cytotoxic activity was separated to yield seven compounds 1–7. Chemical structure analysis using NMR spectroscopy and mass spectrometry confirmed that compound 1 was canophyllol, and compounds 2–7 were garcinielliptones N, O, J, G, F, and garcinielliptin oxide, respectively. Among them, garcinielliptone G (5) showed growth inhibition by causing apoptosis in THP-1 and Jurkat cells derived from human acute monocytic leukemia and T lymphocyte cells, respectively. Apoptosis induced by garcinielliptone G (5) was demonstrated by the detection of early apoptotic cells with fluorescein-labeled Annexin V and increases in cleaved caspase-3 and cleaved PARP protein levels. However, the addition of caspase inhibitor Z-VAD-FMK did not affect growth arrest or apoptosis induction. These results suggest that garcinielliptone G (5) can induce both caspase-3 activation and caspase-independent apoptosis. Therefore, garcinielliptone G (5) may be a potential candidate for acute leukemia treatment.     

差分收敛法对双原子分子高J值转动谱线的预言

为了更精确地预言转动量子数J ≥ 100时双原子分子R支和Q支的振转跃迁谱线, 本文在考虑了转动能级展开式中高阶小项H_v的前提下重新推导出能更好地预言R支和Q支跃迁谱线的物理解析公式. 另一方面, 通过对差分收敛法计算过程的细致分析, 从物理误差的角度提出了一个在没有实验数据作为参照时仍然能有效收敛的重要物理判据. 应用这些新公式和新判据对TiF和CO分子R支振转跃迁谱线和TiF分子Q支振转跃迁谱线进行了研究. 结果表明: 包含高阶小项H_v的新公式的预言结果精度比原有不含H_v的公式的结果提高了一个数量级; 新判据的使用能更有效地减小预言谱线的可能误差, 提高预言结果精度. 最后通过与最小二乘法计算结果的对比, 进一步说明新公式和新判据在预言R支和Q支振转跃迁谱线数据方面的有效性和准确性.     

格栅尾翼(舵)外形参数对气动特性的影响

为研究格栅尾翼/舵主要几何参数--格数、格壁厚度、格壁前缘倒角对其气动特性的影响,在翼高、翼宽、弦长一定的条件下,设计了一组具有不同格数、格壁厚度和格壁前缘倒角的格栅尾翼模型,进行了风洞测力实验,得到了格数、格壁厚度、格壁前缘倒角对气动特性影响的基本规律.基于对实验结果的分析,提出了适于滑翔增程制导武器采用的格栅尾翼气动外形参数的选择方法及对结构设计和材料的要求.     

Preparation of monoclonal antibody based indirect competitive ELISA for detecting 19-nortestosterone residue

19-Nortestosterone (NT) has been illegally used in horse racing to boost physical performance, and in animal husbandry to accelerate weight gain. To monitor the abuse of NT, our goal was to develop a commercial enzyme linked immunosorbent assay (ELISA) kit. For this purpose, hybridomas were prepared by fusing NS0 mouse myeloma cells with splenocytes isolated from immunized BALB/c mouse. Noncompetitive and competitive indirect ELISA were used to screen positive cell clones. To optimize the indirect competiti...     

不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异

以人正常胃黏膜上皮细胞系GES-1和不同恶化程度的人胃癌细胞系(低恶化AGS细胞系、中恶化SGC-7901细胞系、高恶化BGC-823细胞系)为研究对象,采用激光共聚焦显微镜的荧光漂白恢复技术及划痕标记荧光染料示踪技术研究不同恶化程度的胃癌细胞系细胞间隙连接通讯(Gap junctional intercellular communication,GJIC)功能的差异;采用荧光定量PCR技术和间接免疫荧光技术检测间隙连接蛋白(Connexin43,Cx43)基因在mRNA和蛋白水平的表达差异,探求其与胃癌恶性程度的相关性.结果表明,在正常胃细胞系和低、中及高恶化胃腺癌细胞系中,细胞间的GJIC功能随着恶性程度的升高而减弱(AGS、SGC-7901)或消失(BGC-823),且Cx43蛋白及mRNA的表达量随着恶性程度的升高而下调(AGS、SGC-7901)或缺失(BGC-823).这提示胃癌恶性程度与胃癌细胞的GJIC功能显著相关(P<0.05),GJIC在胃癌发生中起重要作用.     

小麦三雌蕊基因Pis1的多效性研究初报

利用小麦三雌蕊突变体与绵阳29杂交,再以绵阳29为轮回亲本,多次回交,并结合人工选择,构建了小麦三雌蕊性状的近等基因系.对小麦三雌蕊突变体,绵阳29以及小麦三雌蕊性状近等基因系的小穗数﹑穗长﹑穗粒数﹑叶长﹑叶宽﹑茎粗﹑千粒重和单株粒重等8个农艺性状进行了分析,结果表明:其中除小穗数和穗长没有显著性差异,其余6个农艺性状均表现出显著性差异.近等基因系在穗粒数、叶长、叶宽、茎粗和单株粒重这5个农艺性状明显高于轮回亲本,而千粒重明显低于轮回亲本.由此可见Pis1基因有增加穗粒数、叶长、叶宽、茎粗和单株粒重的效应,有降低千粒重的副效益.     

METHOD–OF–LINES SOLUTION OF TIME–DEPENDENT TWO–DIMENSIONAL NAVIER–STOKES EQUATIONS

A novel approach to the development of a code for the solution of the time-dependent two-dimensional Navier–Stokes equations is described. The code involves coupling between the method of lines (MOL) for the solution of partial differential equations and a parabolic algorithm which removes the necessity of iterative solution on pressure and solution of a Poisson-type equation for the pressure. The code is applied to a test problem involving the solution of transient laminar flow in a short pipe for an incompressible Newtonian fluid. Comparisons show that the MOL solutions are in good agreement with the previously reported values. The proposed method described in this paper demonstrates the ease with which the Navier–Stokes equations can be solved in an accurate manner using sophisticated numerical algorithms for the solution of ordinary differential equations (ODEs).     

圆偏振飞秒激光脉冲与低压氙气体靶相互作用软X射线辐射实验研究

利用平场光栅谱仪,分别在2和3 kPa的低气压下,测量了脉宽35 fs的圆偏振超强超短激光脉冲与5 mm长氙气体靶相互作用产生的波长在5～60 nm范围内的离子谱线。2 kPa时最强的跃迁为XeⅧ：4d105s(2S_1/2)—4d95s5p(2P_3/2)的17.085 6 nm线,3 kPa时最强的跃迁为11.343 nm的XeⅦ 4d105s2(1S0)—4d95s25f(3P₁)跃迁。两种气压下,Xe均被电离到XeⅦ,XeⅧ,XeⅨ态。     

温度场熵在矩形散热翅片中的应用

在热力学熵和物理场熵的基础上定义了温度场熵的概念,并根据物理场熵的计算方法推导出了温度场熵的计算公式。将此公式应用于矩形散热翅片的研究中,根据翅片表面温度分布函数,计算出翅片表面的温度场熵。在与翅片传热效率比较后可定量地描述矩形散热翅片的热力学完善程度。