时代、民族、个性与绘画的反思

论述了时代、民族、个性之间相辅相成的关系,举出了不同国度、不同历史时代、不同学术流派的具体事例,阐述了在绘画创作中民族传统是土壤、时代是空气、艺术家的个性是推动力的观点,及如何处理三者间的关系,提出艺术家个性化的误区及对策.     

Recognition of ring pairs in the data analysis of Cherenkov detectors

We present a new algorithm for the identification of ring pairs in Cherenkov detectors. The approach combines linear regression methods for the construction of an appropriate reference quantity, methods from statistics for the evaluation of hypotheses and Monte Carlo simulation runs for the tuning of threshold levels.     

基本图素分类的综合判定

本文采用关于手绘图形基本图素的“角度和变量”与“最大距离”综合判定分类法,以流程图符为例,实现了手绘基本图形的在线机器识别.系统的识别率达94.0%～99.5%,无明显等待.     

TNFa在血管内皮细胞ECV304中的靶向表达研究

在缺失了3'LTR U3区内病毒的启动子／增强子序列的逆转录病毒载体pLXSNd中,用血管内皮生长因子受体KDR的特异性启动子调控了TNFa在血管内细胞ECV304中的靶向表达。将构建的载体pLXSN-TNFa,pLXSNd-KDRp-TNFa和空载体pLXSN用PA317细胞包装后获得重组病毒,并用重组病毒分别感染NIH3T3细胞和ECV304细胞,培养物上清的ELISA结果证明,KDR启动子指导的TNFa在KDR阳性细胞ECV304中的表达量为在KDR阴性细胞NIH3T3中的表达量的8倍;而TR指导的TNFa在这两种细胞中的表达无明显差异,实现了TNFa在血管内皮细胞中的靶向表达,这可能为肿瘤基因治疗提供新途径。     

New Chiral Kemp's Acid Diamides for Chiral Amine Recognition by 1 H NMR

Two Kemp's acid diamides were synthesized and applied to chiral amine recognition using ¹H NMR analysis. One derivative based on 1-(1-naphthyl)ethylamine had good chiral recognition of six amines and was useful to determine the optical purity for three amines, i.e., methylbenzylamine, 1-(1-naphthyl)ethylamine and 1- henylpropylamine,however, the cyclohexylethylamine derivative showed little discrimination for the amines studied. Together with the results for alkylamines, it was shown that aromatic structure was important for aromatic shielding anisotropy and –– interactions between host and guest. The structure of the 1-(1-naphthyl)ethylamine derivative in solution was also considered based on ¹H NMR data and computer simulation.     

Chiral recognition in the resolution of enantiomers by GLC

Summary Chiral recognition of many enantiomeric solutes by a chiral amide stationary phase is based mainly on hydrogen bonding. A chiral-recognition-factor CHI is proposed, given by the difference of the enthalpy change in the enantiomer discrimination, standardized with respect to the specific interaction of the solutes with the diamide core of the stationary phase. The r?le of the entropy part is also discussed. By extrapolation of the retention behaviour to elevated temperature, peak inversion of enantiomers is predicted. Presented at the 15th International Symposium on Chromatography, Nürnberg, October 1984     

顺式-二(乙二胺)二硝基合钴(Ⅲ)苹果酸盐的晶体结构及手性识别

合成了标题化合物，并用X-射线衍射法确定了该分子的晶体结构。化合物Δ（λλ），Λ（δδ）－cis－[Co（en）2（NO2）2]2d，l-C4H4O5，Co2C12N12O13H22，Mr=657．94．晶体属单斜晶系，空间群为P21/c，晶胞参数：a=13．969（4），b=6.440（1），c＝14．408（4）A，β=94.37（1）°，V=1292．3A3，Z=2，Dc=1．69g／cm3，μ=105.833cm－1，F（000）=668.最终偏离因子R=0.066，Rw=0．085。结构分析表明晶体存在无序现象，其阴离子C4H4O52-有两种排列方式。R-构型和S-构型的几率各为50％，手性识别是通过Δ构型的阳离子与阴离子R-构型C原子上羟基氧形成氢键相互作用；Λ构型的阳离子与阴离子S-构型C原子上羟基氧形成氢键相互作用而实现的。     

TRANSFER OF FOREIGN GENES BY ELECTROPORATION AND THEIR EXPRESSION IN MAMMALIAN CELLS

Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK-cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK-cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10~(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed celts into nude mice.     

脑神经退行性疾病中的有机化学-朊病毒(疯牛病)中的蛋白物理有机化学和自由基化学

通过关于“普里昂”蛋白病毒疾病的已有临床、医学生理、免疫和化学等方面的现象，讨论了朊病毒当中的部分蛋白氧化损伤和蛋白自由基化学本质。