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Summary This paper demonstrates that the relative standard deviation (RSD) of measurements in a capillary electrophoresis system can
be predicted theoretically from the baseline and the signal shape at low sample concentration. The only requirements for prediction
of the uncertainty are the Fourier transform of the baseline (here 2048 data points) and the observation of signal shape.
The micellar electrokinetic chromatography (MEKC) of acetaminophen and caffeine is taken as an example. The optimum is defined
here as the condition of the lowest RSD or highest precision. The optimum single wavelength is selected from between 220 and
350 nm for the MEKC system equipped with a photodiode array detector. The optimum time domain of signal integration is shown
to be even narrower than the entire signal region, providing an RSD value about half that for integration of the entire region.
The theory is in good agreement with observed RSD values. 相似文献
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在不同操作电压和温度下,SDS-蛋白质在无胶筛分电泳的实验结果表明,电泳力学迁移模型(EMMM)比传统经验式更能准确地反映实验事实.SDS-蛋白质对构象参数(Rc)随热力学温度增加而变化,在288—298K范围内Rc迅速变小,而在298~313K范围内,温度变化对其影响不大.不同的SDS-蛋白质在一定条件下具有相近的活化能,该结论进一步从热力学方面支持了EMMM的一个基本结论,即不同相对分子质量Mr的SDS-蛋白质在同一NGS实验条件下具有相似的形状或构象,并与SDS-蛋白质分子为长棒状这一客观事实相符合. 相似文献
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A new approach based on the effect of pressure in CZE is suggested for acceleration of particle migration in electrophoretic runs resulting in reduction of the analysis time. It provides conditions for studying fast processes in suspensions. The effect of pressure on the migration of silica spheres with average diameters of 100, 150, and 390 nm was studied by CZE at an applied voltage of 25 kV. The particle hydrodynamic behavior was also investigated under the same capillary dimensions and BGE composition. The total particle mobility (excluding the average flow rate) was found to increase with increasing the pressure applied and particle size. The particle migration mechanism explaining the effect of pressure on particle velocity was shown to be almost the same as in wide‐bore hydrodynamic chromatography. It is based on changing radial distribution of particle concentration along the capillary cross section depending on particle diffusivity. On the basis of this mechanism appearance of a zone of negatively charged particles in electropherograms ahead of the marker peak can be explained. 相似文献