胶束电动毛细管色谱法同时测定复方化学消毒剂及日化产品中邻苯二甲醛、对氯间二甲基苯酚及三氯生

建立了邻苯二甲醛(OPA)、对氯间二甲基苯酚(PCMX)和三氯生3种杀菌剂同时分离测定的胶束电动毛细管色谱(MEKC)新方法。详细研究了影响上述3种杀菌剂同时分离与准确定量的因素: 如分离缓冲溶液的浓度及pH,十二烷基硫酸钠(SDS)浓度、样品缓冲溶液等。以40.2 cm (有效长度: 30 cm)×50 μm未涂层熔融石英毛细管为分离柱,20 mmol/L硼砂-80 mmol/L SDS(无需调pH)为分离缓冲溶液,2 mmol/L硼砂-8 mmol/L SDS (含体积分数为10%甲醇)为样品缓冲溶液,检测波长为214 nm。3种杀菌剂的校正峰面积的相对标准偏差(RSD)在1.1%～ 3.8%范围内,迁移时间的RSD均小于0.9%, OPA、PCMX和三氯生的检出限(LOD,信噪比为3)分别为4.0、0.4、0.4 mg/L,定量限(LOQ,信噪比为10)分别为12、1.2、1.2 mg/L,校正峰面积与相应的质量浓度分别在12～2 000 mg/L、1.2～200 mg/L和1.2～200 mg/L范围内具有良好的线性关系,相关系数分别为0.9994、0.9993和0.9995。该法前处理简单,可快速、准确地同时测定3种组分,非常适合常规实验室分析。     

速溶型二氧化氯泡腾片的杀菌效果试验及现场应用

介绍了速溶型二氧化氯泡腾片的杀菌效果,并对现场应用效果进行监测。将泡腾片分别制成50,100,150mg／L的二氧化氯消毒液,对5种常见微生物进行消毒实验,观察消毒效果。将泡腾片制成150mg／L的二氧化氯消毒液,应用于华北制药集团北元有限公司的制药洁净车间,对进入万级洁净区人员的手部、物料传递和万级区环境、设备进行消毒处理,监测现场应用效果。该速溶型二氧化氯泡腾片溶于水后,5min内即可完全溶解、活化;制成的150mg／L二氧化氯消毒液1min就可以杀灭全部受试微生物;活化后的二氧化氯消毒液存放时间超过1d,杀菌能力明显降低。实验得出速溶型二氧化氯泡腾片对细菌具有良好的杀灭效果,但使用时必须现用现配,制成的二氧化氯消毒液存放时间不得超过1d。     

双胶束电动毛细管色谱测定复方化学消毒剂中聚六亚甲基单胍、聚六亚甲基双胍、醋酸洗必泰和苄索氯铵

建立了双胶束电动毛细管色谱(MEKC)分离测定复方化学消毒剂中有效成分聚六亚甲基单胍(PHMG)、聚六亚甲基双胍(PHMB)、醋酸洗必泰(CHA)及苄索氯铵(BTC)的新方法。以50.2 cm(有效长度:40 cm)×50 μm i.d.未涂层熔融石英毛细管为分离柱,20 mmol/L硼砂+30 mmol/L十二烷基硫酸钠(SDS)+5 mmol/L脱氧胆酸钠(SD)+0.8 g/L聚乙二醇 20000为分离缓冲溶液。详细研究了分离缓冲溶液中各组分浓度、样品提取液对分离的影响。4种物质的检出限和定量限均分别为1 mg/L和3 mg/L。4种物质的校正峰面积与相应质量浓度在3~140 mg/L范围内,均具有良好的线性关系,相关系数均大于0.999。回收率在84.1%~109.6%间,相对标准偏差(RSD)均低于6%。用该法测定了11件复方化学消毒剂样品中PHMG、PHMB、CHA和BTC,与产品标识值基本吻合。该法可成功区分单胍与双胍,且操作简单,适用于消毒产品的质量监督。     

Characteristics of Exposure to Chloromethylisothiazolinone (CMIT) and Methylisothiazolinone (MIT) among Humidifier Disinfectant-Associated Lung Injury (HDLI) Patients in South Korea

This study aimed to quantify both chloromethylisothiazolinone (CMIT) and methylisothiazolinone (MIT) dissolved in different product brands and to characterize the exposure to these chemicals among humidifier disinfectant-associated lung injury (HDLI) patients. Both CMIT and MIT dissolved in different humidifier disinfectant (HD) products were quantified using gas chromatography–mass spectrometry. The inhalation level of CMIT and MIT was estimated based on HD-associated factors as reported by HDLI patients. A total of eleven HD products marketed until the end of 2011 were found to contain CMIT and/or MIT. The level of combined CMIT and/or MIT dissolved in these HD products ranged from 12 to 353 ppm. The level varied among HD products and the year of manufacture. The average inhalation levels were estimated to be 7.5, 4.1, and 3.2 μg/m³ for the definite, probable, and possible groups, respectively. If probable and possible groups were collapsed together, the inhalation level of the collapsed group was significantly different from that of the definite group (p < 0.001). All HDLI patients responded as having used HD not only while sleeping, but also as having a humidifier treated with HD within close proximity every day in insufficiently ventilated spaces. These HD use characteristics of patients may be directly/indirectly linked to the HDLI development.