抗酮洛芬多克隆抗体的制备及其间接竞争ELISA检测方法的建立

分别将酮洛芬与牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制得免疫原和包被原,经过免疫新西兰白兔制备多克隆抗体,抗体经纯化后效价为1:128000。使用自制的抗体,建立了测定酮洛芬的间接竞争酶联免疫吸附(ic-ELISA)新方法。ic-ELISA的线性范围为0.010～10.0μg/L,IC50为0.235μg/L,最低检测限为0.0040μg/L,线性回归方程为y=-22.97ρ+104.5(R2=0.980),与布洛芬、双氯酚酸的交叉反应率均小于4%,方法可用于水体中酮洛芬的检测。     

Competitive Binding of Drugs to the Multiple Binding Sites on Human Serum Albumin: A calorimetric study

A multiple-site competitive model has been developed to evaluate quantitatively the equilibrium competition of drugs that bind to multiple classes of binding sites on human serum albumin (HSA). The equations, which are based on the multiple-class binding site model, assume that competition exists at individual sites, that the binding parameters for drug or drug competitor pertain to individual sites, and also that the binding parameters for drug or competitor at any given site are independent of drug or competitor bound at other sites. For the drug-competitor pairs, ethacrynic acid (EA) -caproic acid (C6), -lauric acid (C12), and -palmitic acid (C16), the reaction heat of EA binding to HSA was measured in the absence and the presence of fatty acids at the molar ratio of 3:1 with HSA at pH 7.4 and 37°C by isothermal titration microcalorimetry. The calorimetric titration data induced by the presence of fatty acids were directly compaired to the computer simulation curves by the corresponding multiple-site competititve models, which were precedently calculated from binding parameters of EA and fatty acids. In the case of EA-C12 or -C16 competitive binding, EA binding at the first and the second classes of binding sites on HSA were instantaneously inhibited by C12 or C16, resulting that the binding constant of the first class of binding sites of EA were decreased and that the second class of binding sites on HSA entirely disappeared. In the competition between EA and C6, the first class of binding sites of EA was diminished by C6, resulting in the decrease of the binding constants and the number of binding sites in the first class of EA, whereas, the second class of binding sites was unaffected. The multiple-site competitive model assuming site-site competition could be directly comparable to the calorimetric data and be suitable to account for the competitive processes for drugs bound to the multiple-class of binding sites on HSA.This revised version was published online in November 2005 with corrections to the Cover Date.     

武术进入奥运会的条件分析

自20世纪80年代始,当代中国武术开始走向世界,要走进奥运殿堂,成为正式比赛项目,还存在着一些不利因素.通过不利条件和有利条件的对比,进行了论证和分析,得出武术进入奥运是大势所趋.     

提升中国航空旅游信息业竞争力的对策研究

中国航空市场的飞速发展,使国内外相关领域的知名企业都在积极寻找适当的切入点,中国航空旅游信息业面临严峻挑战,企业必须提升竞争力应对新形式。本文分析了目前国内企业在管理上所存在的问题,并就企业如何通过实现管理的精细化来提升企业竞争力提出了几点建议。     

论竞争情报与企业发展

介绍了竞争情报产生的背景及其特点,阐述了竞争情报获取的途径及其在国内外的应用现状,讨论了竞争情报在企业生存与发展中的重要作用。     

竞争情报服务--图书馆有偿服务的方向

从实施有偿服务的必要性和现状出发,指出竞争情报服务是图书馆有偿服务的最主要的形式,并提出了开展竞争情报服务的措施和方法。     

无标底招标的博弈分析

随着全球经济的飞速发展,在世界范围内已经逐渐形成了一个规模宏大的国际工程市场.对于国际工程项目,世界上普遍采用竞争性招标选择项目承包商.采用这种方式,业主可以在国际市场上找到最有利于自已的承包商,无论是在价格和质量方面,还是在工期和技术方面,都可以满足自己的要求.国际工程项目竞争性招标一般都采用无标底招标,而在我国,为了和国际接轨,也在尝试推行无标底招标.但是,在我国,这种招标方式还很不成熟、不完善,隐含着很多对业主不利的因素.所以,为业主设计一套科学合理的招标,无疑有很大的实用价值和应用前景.利用博弈论作为理论研究工具,建立了无标底招标的博弈模型,从理论的角度证实了无标底招标是优于有标底招标或复合招标的.     

基于高特异性单克隆抗体的间接竞争ELISA检测食品中胭脂红的研究

该研究设计合成了一种胭脂红半抗原,分别采用重氮化法和戊二醛法将半抗原与载体蛋白偶联制备人工抗原,通过免疫Bal b/c小鼠及杂交瘤技术成功筛选制备了胭脂红高特异性单克隆抗体,与苋菜红、柠檬黄等结构类似物无交叉反应.基于该抗体建立了间接竞争酶联免疫分析方法用于检测食品中胭脂红残留.该方法对胭脂红的半抑制浓度(IC50)和...     

建立基于网络的企业竞争情报系统

阐述基于现代网络的企业竞争情报的3个层次,提出了我国有条件的企业应尽快建立基于网络的企业竞争情报的观点。     

市场机制环境下情报研究所与竞争对手比较研究

确定了情报研究所的竞争对手,比较了情报研究所及其竞争对手的优势与劣势,分析了情报研究所发展中面临的困境,提出了建立面向用户的信息服务中心的发展策略。