Microcalorimetry, energetics and binding studies of DNA-dimethyltin dichloride complexes

The interaction of dimethyltin dichloride (Me₂SnCl₂) with calf thymus DNA was studied at 27 °C, pH 7.6 using various techniques including isothermal titration calorimetry (ITC) and UV-Vis, fluorescence and IR spectrophotometries. The binding isotherm and enthalpy curve for Me₂SnCl₂-DNA interaction was a biphasic transition process. This was determined by the analysis of the binding data with the Hill equation. The first phase of the enthalpy curve (exothermic process) was consistent with the first set of binding site, the second phase (endothermic process, less exothermicity) was consistent with second set of binding site from the cited interactions. Our results showed that the first set of binding sites is occupied by one mole of ligand bound per near 1 base pair of DNA. The DNA-ethidium bromide (EB) complex, in the presence of Me₂SnCl₂, caused the quenching of the fluorescence emission. The Scatchard plots illustrated a non-intercalating manner for such quenching. The DNA-EB complex results indicated that the binding of Me₂SnCl₂ is with the phosphate groups of DNA at low ligand concentrations (<9 mM). This was confirmed with the IR spectrophotometric spectra. However, the binding at higher ligand concentrations (>9 mM) was with the base groups of DNA. Therefore, these results suggest that the Me₂SnCl₂ binding to DNA at low concentrations occurs through an outside interaction by an exothermic process. However, the partial unfolding of the DNA caused at higher concentrations of Me₂SnCl₂ is through an endothermic process involving interactions with the base groups.     

Surface area of papermaking woodpulps used by the British paper industry

The surface area was determined for various papermaking woodpulps: bleached eucalyptus globulus sulphate pulp; bleached eucalyptus grandis sulphate pulp; bleached betula verrucosa sulphate pulp; bleached pine/spruce sulphate pulp; bleached pine/spruce sulphate pulp fines. The method of negative adsorption was used which gives an effective wet surface area. By looking at negative adsorption data more closely, some inference can be made about the morphology of the substrate.Glossary of symbols B (e²/2kT)^1/2 - ¯C concentration of fibre per unit volume of pulp - 1 - ¯¯c - h angle between pore and the axis of the bed in a permeable material - K permeability coefficient - k Boltzmann constant - k ₀ shape factor for pores in a permeable material - n _i concentration of co-ion in the bulk solution - n _i experimentally observed increase in concentration of co-ion in the bulk solution - S surface area - S _t wS - S ₀ surface area per unit volume of permeable material - V _t total volume of solution - V excluded volume - V _s excluded volume accessible to water but not to ions - V _obs experimentally observed excluded volume - w weight of dry fibre - ¯ effective volume of fibres in a permeable pad - surface potential at outer Helmholtz plane     

浅谈开放教育与图书馆服务

广播电视大学图书馆是电大的文献信息中心,随着开放教育这一新的教育模式的出现,图书馆也应在整体上做出相应的改革,树立“以人为主体”的服务理念。未来图书馆事业发展的核心是信息增值服务和人本服务。     

中国小冰期区域气候演化研究

在我国不同地区通过不同代用资料如冰芯、树轮、孢粉、湖泊沉积物、历史文献记录等恢复的小冰期气候的基础上, 分析总结了小冰期我国气候演化的区域分异特征,并探讨其原因机制,力图为未来气候变化提供参考。     

浅论新型师生关系的建构

随着素质教育的不断推进和深化，建立新型的师生关系在教学改革中显得尤为重要。通过师生相互沟通使学生参与教学决策，通过了解学生需求以融合师生感情并增强学生的主体意识，从而在更高的层次上激发学生参与教学活动和教学管理的积极性、主动性，使学生能够开放思维的空间，激发创新灵感，并真正成为学习的主人。     

陕西省人均粮食与人均耕地变化研究

对陕西省人均粮食及人均耕地变化进行了时空差异分析,结果表明:(1)近50余年的绝大多数年份为人均粮食占有量的递增期,且不同时段的增幅不同;粮食增长率大于人口增长率的时期,对应人均粮食的增长时期;(2)1978—1998年绝大多数地、市人均粮食递增,且地域差异明显;(3)近50多年来全省耕地面积总体逐年递减,近10年来减少之势加剧;同期人口数量的持续增长,造成人均耕地面积急剧减少.相关回归分析显示:各地、市人均粮食绝对增量与其相对增量之间呈正相关,人均粮食增加量占粮食增量的份额整体偏低;提高粮食生产水平及控制人口是增加全省人均粮食占有量的重要途径.     

论FDI对西部制度变迁的影响及对策

本文首先对我国西部地区利用FDI的现状进行了描述 ,然后运用回归分析的方法分析了FDI和制度变迁的关系 ,指出FDI是影响区域制度变迁的重要因素 ,FDI在区域分布上的不均衡严重制约着西部地区制度变迁的进程。西部地区应该加快体制改革的步伐 ,改善投资环境 ,吸引FDI ,进而通过FDI带动西部地区制度变迁的进程 ,最终形成FDI和制度变迁相互推动、相互促进的良性循环。     

Temperature variations on the Tibetan Plateau over the last two millennia

The paleoclimate data recovered from ice cores,tree rings and lake sediments indicate regional features of cfimatic change on the Tibeta n Plateau (TP) during the last 2000 years. The composite temperature reconstructions in-dicate that several main climatic episodes, such as the “LittleIce Age“ between 1400 and 1900, the “Medieval Warm Pe-riod“ in 1150-1400, a less warm period in 800-1100, and an earlier cold period between the 3rd and 5th centuries,occurred in the TP. In addition, temperature varied from region to region. The period from AD 800 to 1100, which waswarm in northeastern TP, was contemporaneous with cool-ing in the western and southern TP. The southern TP ex-perienced warming between 1150 and 1400. For western TP,the δ^18O records of the Guliya ice core indicate that the pe-Hod 1250-1500 witnessed a clear warming. Large-scaletrends in the temperature history from northeastern TP aremore similar to those in eastern China than are the trendsfrom the Guliya ice cap far to the west and southern TP. The most prominent similarities between the temperature varia-tions of the TP and eastern China are such cold phases as 1100-1150, 1500-1550, 1650-1700 and 1800-1850, andthe latter three cold events match with three widespreadg lacial advances which occurred on the TP during the Little Ice A2e.     

并联电压负反馈输入、输出电阻的讨论

电路中引入负反馈,使输入、输出电阻均发生变化,并且输入、输出电阻对放大电路有很大的影响.从并联电压负反馈的角度,对输入电阻和输出电阻给出了下列4种方法的定量讨论.     

烟草中多酚氧化酶的生理生化特征及其活性控制的研究

对烟草叶中的多酚氧化酶(PPO)进行了部分纯化，对比了叶中PPO在苗期和田间的活性差异，研究了其活性变化规律，发现生长旺盛期PPO活性高于其他时期，研究了烟叶在不同水势条件下PPO活性变化规律，发现水势过高、过低，PPO活性均较低，在一872．78kPa水势条件下，PPO活性最高，所以细胞中的含水量是影响PPO活性的因素之一．研究了PPO在35℃、40℃、45℃、50℃、55℃、60℃、65℃、70℃和75℃等烘烤温度条件下的热敏感性，发现：酶的最适反应温度为35℃；温度由35℃升至75℃，活性逐渐下降，在每一温度下保持5min，活性趋于稳定，用回归法得出了烟叶中PPO的下降百分数与温度之间的关系式．用双倒数作图法比较了NaDiCa、EDTA、巯基乙醇和硫脲4种常用抑制剂对烟叶中PPO活性的抑制强度，结果表明：同一浓度下它们对PPO的抑制强度依次递减，而NaDiCa抑制效率明显高于其他3种抑制剂；CuCl2可以促进PPO活性，用回归法得出了活力增加百分数与二价铜离子浓度的关系式。