Analysis of commercial dyes by capillary column supercritical fluid chromatography

High resolution separations of selected commercial azo, aniline, and anthraquinone dyes by capillary column supercritical fluid chromatography (SFC) are demonstrated. Supercritical n-pentane was used as a mobile phase and provided efficient separations of multi-functional, polar disperse dyes with molecular weights up to approximately 700 daltons.     

J_μ型高效凝胶色谱填料的色谱性质

研究高效聚苯乙烯型凝胶色谱填料的孔径及孔径分布、粒度及粒度分布、凝胶的孔容、比分离度等几个主要物理性能指标。     

涂层剖析方法探讨

进行纺织品或纸张上涂层的剖析,关键是对涂层进行恰当的分离。通过对进口高光泽纸涂层的剖析,获得结论是:应用多种溶剂连续抽提和柱层析分离法能取得较满意的剖析结果。本方法对于纺织品涂层的剖析也有参考价值。     

酮类化合物在含氟和非含氟整体柱上电色谱保留行为比较

利用全氟辛基甲基丙烯酸酯为单体之一,通过原位聚合的方式,制备了1种新型的含氟有机聚合物毛细管电色谱整体柱,并在相同的实验条件下对几种酮类化合物在含氟和非含氟有机聚合物整体柱上的保留行为进行了比较.结果表明,在含氟有机聚合物整体柱上,酮类化合物的保留能力明显减弱,选择性也有所提高.     

推广梁表柱表简化结构设计

从比较传统设计方法与现代化的微型计算机辅助设计方法入手，阐述了在多导与高层建筑结构设计中推广梁表柱表，简化结构设计的可行性、优越性和必然性。     

非极性SE－52交联毛细管柱的研制

采用静态法以臭氧及过氧化二异丙苯（ＤＣＵＰ）为引发剂制备非极性固定液ＳＥ－５２弹性石英毛细管交联柱，制得的柱子具有柱效高、内壁惰性好、耐溶剂冲洗等特点。柱子适用于烷烃类样品分离。方法简便，适用于一般实验室。     

高效液相色谱法测定蜂王浆中的10－羟基癸烯二酸

用自行合成的乙烯基交联氨基键合固定相建立了不经分离，只需稀释就可进样定量分析蜂王浆中１０－羟基癸烯二酸（１０－ＨＤＡ）的高效液相色谱方法．流动相：含１０％甲醇的０．０５ｍｏｌ／Ｌ磷酸缓冲液（ｐＨ４．１～４．３）；检测器：紫外吸收；波长：２１３ｎｍ．该方法简单，快速，用于测定蜂王浆及其制品中的１０－ＨＤＡ，标准偏差小于１．５％，回收率为９５％～１０５％．     

Retention prediction of polycyclic aromatic hydrocarbons in reversed phase liquid chromatography with various mobile phase compositions and column temperatures

Summary The relationship between the logarithmic capacity factor measured in reversed-phase liquid chromatography and the operating conditions including the mobile phase composition and the column temperature is investigated. The strategy described herein can offer the possibility to predict the retention of polycyclic aromatic hydrocarbons without any experiments and standard materials, by utilizing equations describing the relationships between retention, temperature, mobile phase composition and physicochemical properties of the solutes previously stored in the program of the microcomputer-assisted retention prediction system.This concept is one of the most promising techniques for the optimization of the separation conditions in reversed-phase liquid chromatography.