氯霉素残留胶体金免疫层析快速检测法的研制

建立一种快速、简易的检测样品中氯霉素残留含量的胶体金免疫层析法.将抗氯霉素单克隆抗体-胶体金复合物包被在胶体金结合垫上,并将人工合成的氯霉素抗原包被在硝酸-醋酸纤维素薄膜表面作为检测线(T线),其与待测样品中氯霉素竞争结合胶体金标记的氯霉素单克隆抗体,并能以颜色直观显示检测的结果.用GICA与ELISA试剂盒对比检测了...     

核酸适配体氯化血红素比色法检测牛奶中的氯霉素

利用一种基于适配体氯化血红素(hemin)比色分析法快速检测牛奶中的氯霉素(chloram-phenicol,CAP)含量.设计两条DNA链,其中一条为CAP的核酸适配体,在其3′端修饰氯化血红素;另一条为CAP核酸适配体的互补链(cDNA),在其5′端修饰hemin.当体系中无CAP时,两条DNA单链杂交形成DNA双...     

氯霉素的单扫描示波极谱法测定

用单扫描示波极谱法研究氯霉素的电化学行为。在0.3mol/LKCl(pH=7.30)底液中,氯霉素在汞电极上有一线性扫描还原峰,其峰电位为Ep=-0.62V(vs.SCE)。峰电流与氯霉素浓度在1×10-7mol/L～9×10-6mol/L范围内呈现良好的线性关系(其线性相关系数r=0.9992),氯霉素的检出限可达5.0×10-8mol/L。该法用于模拟尿样的测定和氯霉素片剂含量的测定,结果令人满意。     

超高效液相色谱-串联质谱法检测禽畜肉中的氯霉素残留

 研究了一种针对禽畜肉中氯霉素残留量的超高效液相色谱-串联质谱联用分析方法.本方法采用乙酸乙酯萃取禽畜肉中的氯霉素,提取液氮吹至干后用超纯水溶解样品残渣,然后用HLB小柱进行固相萃取,经过净化的样品通过超高效液相色谱进行分离,然后用电喷雾质谱负离子多反应监测模式对氯霉素进行检测.该方法的检出限为0.0025μg/kg,添加水平为0.1~5.0μg/kg时,加标回收率为75.8%~82.5%,相对标准偏差小于10%.该方法具有灵敏度高、选择性好、分析时间短等优点.     

Analyses of Chloramphenicol in Biological Samples by HPLC

Abstract

Chloramphenicol (CAP) is the drug of choice for the treatment of typhoid fever and other diseases and is consumed by millions of people. Chloramphenicol is a broad-spectrum antibiotic, but it has serious side effects and high risk of anemia if taken continuously. The drug persists for a long time in the human body and is likely to damage to the liver, kidneys, and red blood cells, even at low concentrations. From the human health point of view, analysis of low levels of this drug in biological samples is important. This article present an overview of the analysis of CAP in biological samples by high-performance liquid chromatography (HPLC). In addition, the available methods are compared in terms of their applications, efficiencies, and effectiveness.     

氯霉素对斑马鱼幼鱼发育及免疫毒性的研究

以野生型及转基因Tg(lyz:EGFP)斑马鱼幼鱼为研究对象,研究氯霉素对斑马鱼幼鱼的发育毒性及对免疫细胞的影响。用不同浓度的氯霉素分别处理72 h,观察斑马鱼幼鱼的生存率、畸形率和形态变化,以及标记绿色荧光免疫细胞在斑马鱼幼鱼中的数量。结果显示,随着氯霉素浓度(≥100 mg/L)的升高和暴露时间的延长,斑马鱼的存活率和孵化率降低,死亡率升高;氯霉素对斑马鱼幼鱼的致畸作用,主要表现为心包水肿、卵黄囊水肿、脊柱弯曲;通过对Tg(lyz:EGFP)品系的观察,发现氯霉素(≥75 mg/L)可以降低斑马鱼幼鱼免疫细胞的数量。研究表明,氯霉素对斑马鱼幼鱼的致畸和致死作用具有剂量、时间依赖性,并可以导致斑马鱼幼鱼的免疫细胞数量减少,具有免疫毒性。     

Surface‐enhanced Raman scattering (SERS) spectra of chloramphenicol in Ag colloids prepared by microwave heating method

Ag colloids were prepared by the microwave heating method. The UV‐visible spectrum and the transmission electron microscopy (TEM) were employed to characterize the Ag colloids. The surface‐enhanced Raman scattering (SERS) spectra of chloramphenicol (CAP) in Ag colloids were successfully recorded. The Raman spectra of CAP were recorded with good concordance comparing to the theoretical results calculated by the Gaussian'98 program. CAP molecules on the Ag surface are oriented tilted to the particle surface via C20‐O23, N‐O13, and N‐O23, which played an important role in the SERS effect. Copyright © 2009 John Wiley & Sons, Ltd.     

Tuning Selectivity and Sensitivity of Mixed‐polymeric Tetraruthenated Metalloporphyrins Modified Electrodes as Voltammetric Sensors of Chloramphenicol

Mixed Ni?Co tetraruthenated porphyrin films were successfully electropolymerized on glassy carbon electrodes in alkaline solutions (pH=13) of NiTRP and CoTRP, giving excellent electrocatalytic responses for determination of the antibiotic drug chloramphenicol. Electrodes with four different compositions were obtained by varying the ratio of NiTRP and CoTRP in the deposition solution. For the characterization of these materials, SEM, ICP‐MS, EIS and CV were explored. The porphyrin modified glassy carbon electrodes presented two oxidation peaks for chloramphenicol (except for Ni‐25). The potential shifted to lower values as the percentage of CoTRP increased decreasing its oxidation potential in more than 200 mV for Ni‐50 as compared to Ni‐100. Interestingly, the materials with cobalt content larger than 50 % did not show the typical catalytic response of the Ni^IIIOOH species, but rather showed an increase in current at potentials larger than 0.4 V, demonstrating the key role played by nickel in this type of materials. The composition of polymeric mixed porphyrin materials influenced the oxidation current response of both redox waves, resulting in different sensitivities on the calibration curves.     

离子阱时间串联质谱测定食品中的氯霉素

本文采用离子阱时间串联质谱法测定食品中氯霉素残留.样品经混合溶剂提取,C18小柱结合硅胶柱净化后,用双三甲基硅基三氟乙酰胺(BSTFA)衍生后,用GC/MS/MS测定.本法准确、稳定,检测灵敏度可达0.1 μg/kg.