过采样∑-△ADC的原理及单片机实现方法

论述了过采样 Σ- ΔADC的基本原理及结构 ,分析了 Σ- Δ调制器的频域传输特性和系统的信噪比 ,给出了实现不同的 A/D转换精度必须满足的条件和用单片机实现 Σ- ΔADC的具体方法和电路 .实际使用表明 ,该方法测量结果可靠 ,具有实用价值     

一种简易数控直流电流源的设计

介绍了一种简易数控直流电流源的设计。该电流源主要由数字控制部分和恒流源产生电路部分组成。其中单片机为系统的核心控制器,此外还包括键盘输入模块、LCD液晶显示模块以及DA转换模块。在电流源中主通路上,选了两个功率比较大的模拟器件LM338和TIP122,能稳定可靠地输出2A的最大电流。     

微生物基因芯片鉴定及再生利用

生物芯片是一种重要的生物分子检测方法,在科研和医学应用领域被广泛关注。该文以大肠杆菌和黄单胞菌检测为例研究微生物的基因芯片鉴定方法和生物芯片的再生使用价值,采用一种自主研制的新型微阵列芯片扫描仪对微生物鉴定和生物芯片的重复利用开展理论与实验研究。结果表明基因芯片鉴定微生物是非常可靠的,所设计的生物分子探针与芯片表面修饰分子的结合很牢固,5次重复杂交清洗后依然可以产生良好的荧光信号,从而验证了再生芯片使用的可行性。为了提高生物芯片的重复使用次数,论文还对生物芯片的设计与荧光标记方法进行了分析讨论。     

单片机控制IGBT逆变埋弧焊机的研制

针对IGBT逆变埋弧焊机设计了基于单片机80C196KC的控制系统,介绍了系统的软、硬件组成和控制原理.焊接电源采用PWM控制技术,通过电流反馈PI控制算法调节电源输出外特性.送丝及行走小车调速系统采用MOSFET型开关电源,采用电弧电压反馈PI算法,实现了送丝速度的闭环控制.研制的焊机具有参数预置、数字显示和自动引弧收弧等功能.试验结果表明,样机性能稳定,工作可靠,达到了设计要求.     

Sample Injection for Capillary Electrophoresis on a Micro Fabricated Device/On Chip CE Injection

Abstract

Reproducible injection for capillary electrophoresis on a micro device/Lab on chip is not an easy task. Different injection designs (e.g., T‐type, double T‐type, cross) and different injection modes have been applied (pinched injection, gated injection, optically gated injection, pressure/pneumatic injection, double L injection) with various analytes. Problems and properties of the different modes are described.     

Electrokinetic characterization of hybrid NOA 81-glass microchips: Application to protein microchip electrophoresis with indirect fluorescence detection

A low-cost and straightforward hybrid NOA (Norland optical adhesive) 81-glass microchip electrophoresis device was designed and developed for protein separation using indirect fluorescence detection. This new microchip was first characterized in terms of surface charge density via electroosmotic mobility measurement and stability over time. A systematic determination of the electroosmotic mobility (μ_eo) over a wide pH range (2–10) and at various ionic strengths (20–50 mM) was developed for the first time via the neutral marker approach in an original simple frontal methodology. The evolution of μ_eo was proved consistent with the silanol and thiol functions arising from the glass and the NOA materials, respectively. The repeatability and reproducibility of the measurements on different microchips (RSD < 14%) and within 15 days (less than 5% decrease) were successfully demonstrated. The microchip was then applied for the efficient electrophoretic separation of proteins in a zonal mode coupled with indirect fluorescence detection, which is, to our knowledge, the first proof of concept of capillary zone electrophoresis in this hybrid microsystem.     

微流控纸芯片的加工及其分析应用的实验设计*

设计了一个使用喷墨打印法加工微流控纸芯片的实验,在经烷基烯酮二聚体(AKD)的正己烷溶液浸泡过的滤纸上,以三乙醇胺溶液为打印墨水,用喷墨打印机打印出设计好的芯片图案。滤纸加热后,打印区域呈现亲水性,非打印区域为疏水性,从而制备出纸芯片,用该纸芯片通过数字比色法实现了亚硝酸根离子的定量测定。该实验不使用昂贵仪器设备,易普及。通过实验,促使学生了解微流控芯片这一前沿科学技术,锻炼学生细致、灵巧的动手能力,激发学生科技创新活力。     

微流控纸芯片在环境分析检测中的应用

近年来,微流控纸芯片由于低成本、便携化、检测快等优点,在需要快速检测的环境分析领域中展现出了巨大的应用前景.该综述从微流控纸芯片在环境分析中的应用角度,总结归纳了微流控纸芯片在环境分析中的最新研究进展,并展望了其在未来的发展趋势与挑战.论文内容引用150余篇源于科学引文索引(SCI)与中文核心期刊中的相关论文.该综述包...     

Microwave-assisted synthesis of colorimetric and fluorometric dual-functional hybrid carbon nanodots for Fe3+ detection and bioimaging

Carbon nanodots (CDs) based fluorescent nanoprobes have recently drawn much attention in chemo-/bio-sensing and bioimaging. However, it is still challenging to integrate the colorimetric and fluorometric dual readouts into a single CD. Herein, novel hybrid CDs (HCDs) are prepared by a simple microwave-assisted reaction of citric acid (CA), branched polyethyleneimine (BPEI) and potassium thiocyanate (KSCN). As-prepared HCDs show extraordinary properties, including excitation-dependent emission, satisfactory fluorescence quantum yield (46.8%), excellent biocompatibility and optical stability. Significantly, the fluorescence intensity at 450 nm exhibits linear correlation over the Fe³⁺ concentration from 1 μmol/L to 150 μmol/L with a detection limit (LOD) of 52 nmol/L. Meanwhile, the solution color changes from colorless to orange, and the absorbance at 460 nm increased linearly with Fe³⁺ concentration ranging from 0.02 mmol/L to 5 mmol/L (LOD: 3.4 μmol/L). All the evidence illustrates that the HCDs can be conditioned for specific Fe³⁺ sensing with colorimetric and fluorometric dual readouts, which has also been verified with paper-based microchips. The possible mechanism is attributed to the specific interactions between surface functional groups on the HCDs and Fe³⁺. Additionally, the HCDs are successfully applied in sensing Fe³⁺ in wastewater and living cells, demonstrating its potential applications in future environment monitoring and disease diagnosis.