氮源水解液对链霉菌转谷氨酰胺酶合成的影响

研究了常压及高压条件下以豆饼粉、棉籽粉酸水解液作氮源,对链霉菌(Streptomyces)HS-1产转谷氨酰胺酶的影响．初步试验高压条件下以所得豆饼粉水解物作氮源,链霉菌(streptomyces)HS-1产转谷氨酰胺酶的酶活可达3．86μmol／(min·mL)．对高压条件下豆饼粉的水解条件进行确定：以3mol／L的盐酸水解4h所得水解物的发酵酶活较高,为5．17μmol／(min·mL),且进一步实验以蛋白胨与豆饼粉水解液作混合氮源时发酵酶活较单氮源高．     

花生麸中膳食纤维含量的测定

采用Prosky法测定了花生麸中膳食纤维的含量，测定结果显示花生麸中水溶性膳食纤维含量为9．26％，水不溶性膳食纤维含量为39．58％。     

从油茶饼提取皂素的工艺研究

本文报导用有机溶剂法从油茶饼提取、纯化皂素的工艺,以及油茶籽皂素的主要技术指标的测定方法与测定结果。通过与国内外同类技术及产品的比较,说明油茶籽皂素的质量达到国内外同类产品的水平。     

基于高通量测序对四川怀远特色发酵食品微生物群落结构分析

为了研究四川怀远特色传统发酵食品的微生物多样性,利用Illumina HiSeq 2500高通量测序平台,对当地豆腐帘子和冻糕的微生物群落结构进行了系统分析.结果表明,豆腐帘子的菌群主要隶属于变形菌门(Proteobacteria)和担子菌门(Basidiomycota),检测出40个细菌属和21个真菌属,主要优势微生物是Acinetobacter和Trichosporon.冻糕的菌群主要隶属于厚壁菌门(Firmicutes)和子囊菌门(Ascomycota),检测出36个细菌属和23个真菌属,主要优势微生物是Lactobacillus和Kazachstania.     

漳平水仙饼茶中总黄酮提取工艺优化研究

采用超声波辅助提取漳平水仙饼茶总黄酮,在单因素试验的基础上,选取乙醇浓度、液料比、超声功率和提取时间为自变量,总黄酮的得率为响应值,应用Box-Behnken中心组合试验设计建立数学模型,进行响应面分析。结果表明:最优的提取工艺条件为:乙醇体积分数62%,液料比25∶1(m L/g),超声功率400 W,提取时间36 min,水仙饼茶总黄酮得率为3.125 mg/g与模型预测值3.136 mg/g基本一致。     

Triacylglycerols and Other Lipids Profiling of Hemp By-Products

Hemp seed by-products, namely hemp cake (hemp meal) and hemp hulls were studied for their lipid content and composition. Total lipid content of hemp cake and hemp hulls was 13.1% and 17.5%, respectively. Oil extraction yields using hexane, on the other hand, were much lower in hemp cake (7.4%) and hemp hulls (12.1%). Oil derived from both hemp seeds and by-products were primarily composed of neutral lipids (>97.1%), mainly triacylglycerols (TAGs), determined by SPE and confirmed by NMR study. Linoleic acid was the major fatty acid present in oils derived from hemp by-products, covering almost 55%, followed by α-linolenic acid, covering around 18% of the total fatty acids. For the first time, 47 intact TAGs were identified in the hemp oils using UPLC-HRMS. Among them, TAGs with fatty acid acyl chain 18:3/18:2/18:2 and 18:3/18:2/18:1 were the major ones, followed by TAGs with fatty acid acyl chain of 18:3/18:3/18:2, 18:2/18:2/16:0, 18:2/18:2/18:1, 18:3/18:2.18:0, 18:2/18:2/18:0, 18:2/18:1/18:1 and 18:3/18:2:16:0. Besides TAGs, low levels of terpenes, carotenoids and cannabidiolic acid were also detected in the oils. Moreover, the oils extracted from hemp by-products possessed a dose-dependent DPPH radical scavenging property and their potencies were in a similar range compared to other vegetable oils.     

疏水型色谱饼对人血清蛋白质组学样品的快速分离与制备

建立了疏水型色谱饼(10 mm×20 mm i.d.)与反相色谱(RPLC)离线二维色谱快速分离制备人血清蛋白质组学样品,并用基体辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)进行检测的方法。以4种标准蛋白质的稀溶液为模型进行分离富集,得到细胞色素c(Cyt-c)与肌红蛋白(Myo)的检出限均为1 pmol/μL,溶菌酶(Lys)和胰岛素（Ins）的检出限为0.1 pmol/μL。将此方法用于人血清蛋白质组学样品的分离与制备,随着血清处理量的增大,质谱可检出的组分数目与信号强度均增加,当血清处理量达到1.0 mL时,可检出低丰度蛋白质或多肽285个(相对分子质量均在15000以下)。研究中将1 μg Cyt-c加入到0.5 mL血清中,用上述方法在分离富集低丰度Cyt-c上取得了很好的效果。结果表明,采用疏水型色谱饼与反相色谱联用技术不仅可对血清样品中低丰度蛋白质进行有效的分离和富集,而且一次样品的处理量大,可显著提高低丰度蛋白质的分析、检测水平。     

The Effect of Precipitation pH on Protein Recovery Yield and Emulsifying Properties in the Extraction of Protein from Cold-Pressed Rapeseed Press Cake

Rapeseed is the second most cultivated oilseed after soybean and is mainly used to produce vegetable oil. The by-product rapeseed press cake is rich in high-quality proteins, thus having the possibility of becoming a new plant protein food source. This study aimed to investigate how the precipitation pH affects the protein yield, protein content, and emulsifying properties when industrially cold-pressed rapeseed press cake is used as the starting material. Proteins were extracted under alkaline conditions (pH 10.5) with an extraction coefficient of 52 ± 2% followed by precipitation at various pH (3.0–6.5). The most preferred condition in terms of process efficiency was pH 4.0, which is reflected in the zeta potential results, where the proteins’ net charge was 0 at pH 4.2. pH 4.0 also exhibited the highest protein recovery yield (33 ± 0%) and the highest protein concentration (64 ± 1%, dry basis). Proteins precipitated at pH 6.0–6.5 stabilized emulsions with the smallest initial droplet size, although emulsions stabilized by rapeseed protein precipitated at pH 5.0–6.0 showed the highest emulsion stability at 37 °C for 21 days, with a limited layer of free oil. Overall, emulsion stabilized by protein precipitated at pH 5.0 was the most stable formulation, with no layer of free oil after 21 days of incubation.     

ZPH—蛋白粉饲料的研制

ZPH——蛋白粉以棉饼为主要原料经脱毒精制而成。其脱毒率为89.6-90.4%,粗蛋白含量为46.76-56.96%,平均为50.86%,在原基础上提高9.66-19.6%,平均提高13.33%,得率为52-66.5%平均为59.78%。该项研究和产品对缓解国内蛋白质饲料资源不足有较大的现实意义和经济意义。     

城市污泥低压氧化的中试研究

研究是国内首次较大规模的城市污泥低压氧化中试试验、结果表明,在170～185℃温度的反应温度下,在纯氧存在条件下,低压氧化可以取得较好的污泥减容改性效果、污泥上清液中的化学需氧量(COD)由平均l972mg/L上升至8000～12000mg/L,悬浮固体(SS)溶解率20％～60％,脱水泥饼的固体质量分数为45％～50％,热值约为8000kJ／kg.