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21.
O. Schullian 《Molecular physics》2019,117(21):3076-3087
ABSTRACTDirect simulation Monte Carlo (DSMC) models have been successfully adopted and adapted to describe gas flows in a wide range of environments since the method was first introduced by Bird in the 1960s. We propose a new approach to modelling collisions between gas-phase particles in this work – operating in a similar way to the DSMC model, but with one key difference. Particles move in a mean field, generated by all previously propagated particles, which removes the requirement that all particles be propagated simultaneously. This yields a significant reduction in computation effort and lends itself to applications for which DSMC becomes intractable, such as when a species of interest is only a minor component of a large gas mixture. 相似文献
22.
一个可靠机器,一个不可靠机器和一个缓冲库构成的系统的定性分析 总被引:9,自引:6,他引:9
郭卫华 《数学的实践与认识》2002,32(6):939-943
本文用纯分析的方法给出了一个可靠机器 ,一个不可靠机器和一个缓冲库构成的系统解的存在唯一性证明 相似文献
23.
运用灰色系统GM(1,1)模型进行预测时,模拟精度和预测精度是关注的焦点.对原始数据构造缓冲算子和进行完整的前期检验,运用等维灰数递补的动态建模方法,针对误差建立残差修正模型,都可以提高预测的可信度.通过归纳建模各阶段可能出现的典型问题以及解决方法,以期对建立优化模型有所帮助.最后建立GM(1,1)模型预测天津滨海机场货邮吞吐量. 相似文献
24.
针对ATM网络中不同业务的需要,提出了一种采用FIFO机制的易于实现的单队列缓存分配方案,但该方案为了获得较高的链路利用率就需要较大的缓存空间·因此进一步提出了旨在减少信息对缓存的占用的多队列链路速率分配算法,并求解了链路速率的分配规则表达式·理论分析表明,该算法能够在一定程度上克服单队列缓存分配算法对缓存空间的耗费,并保证链路速率的合理分配· 相似文献
25.
《Analytical letters》2012,45(6):451-465
Abstract The influence of the composition of buffer solutions on fluoride analyses by the fluoride ion selective electrode was studied. Residual fluoride content of reagents was observed to restrict the use of some buffers and reagents in low-level work. The optimum pH for low-level fluoride determinations was found to be around 5, which can be maintained by a sodium acetate-acetic acid buffer. Of the complexing agents studied, citrate was observed to be the most efficient masking agent for aluminum, but this ability depended strongly on ligand concentration. Citrate was also effective in masking iron (III) and magnesium ions. Tris (hydroxymethyi) aminomethane showed a similar ability to complex aluminum at a pH around 8. However, at this pH the hydroxyde ion interferes in fluoride analysis below 0.1 ppm [fbar]. 相似文献
26.
Sulfated glycosaminoglycans were labeled with biotin to study their interaction with cells in culture. Thus, heparin, heparan sulfate, chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate were labeled using biotin-hydrazide, under different conditions. The structural characteristics of the biotinylated products were determined by chemical (molar ratios of hexosamine, uronic acid, sulfate and biotin) and enzymatic methods (susceptibility to degradation by chondroitinases and heparitinases). The binding of biotinylated glycosaminoglycans was investigated both in endothelial and smooth muscle cells in culture, using a novel time resolved fluorometric method based on interaction of europium-labeled streptavidin with the biotin covalently linked to the compounds. The interactions of glycosaminoglycans were saturable and number of binding sites could be obtained for each individual compound. The apparent dissociation constant varied among the different glycosaminoglycans and between the two cell lines. The interactions of the biotinylated glycosaminoglycans with the cells were also evaluated using confocal microscopy. We propose a convenient and reliable method for the preparation of biotinylated glycosaminoglycans, as well as a sensitive non-competitive fluorescence-based assay for studies of the interactions and binding of these compounds to cells in culture. 相似文献
27.
Zhi Zeng Marina Hincapie Brian B. Haab Samir Hanash Sharon J. Pitteri Steven Kluck Jason M. Hogan Jacob Kennedy William S. Hancock 《Journal of chromatography. A》2010,1217(19):3307-3315
Protein glycosylation represents one of the major post-translational modifications and can have significant effects on protein function. Moreover, changes in the carbohydrate structure are increasingly being recognized as an important modification associated with cancer etiology. In this report, we describe the development of a proteomics approach to identify breast cancer related changes in either concentration and/or the carbohydrate structures of glycoprotein(s) present in blood samples. Diseased and healthy serum samples were processed by an optimized sample preparation protocol using multiple lectin affinity chromatography (M-LAC) that partitions serum proteins based on glycan characteristics. Subsequently, three separate procedures, 1D SDS-PAGE, isoelectric focusing and an antibody microarray, were applied to identify potential candidate markers for future study. The combination of these three platforms is illustrated in this report with the analysis of control and cancer glycoproteomic fractions. Firstly, a molecular weight based separation of glycoproteins by 1D SDS-PAGE was performed, followed by protein, glycoprotein staining, lectin blotting and LC–MS analysis. To refine or confirm the list of interesting glycoproteins, isoelectric focusing (targeting sialic acid changes) and an antibody microarray (used to detect neutral glycan shifts) were selected as the orthogonal methods. As a result, several glycoproteins including alpha-1B-glycoprotein, complement C3, alpha-1-antitrypsin and transferrin were identified as potential candidates for further study. 相似文献
28.
Wang-bing Zhang Xin-an Yang Yuan-yuan Ma Hai-xia Zhu Shang-bing Wang 《Microchemical Journal》2011,97(2):201-206
The determination of trace concentrations of Hg in water samples by the use of electrolytic cold vapor generation (ECVG) system and AFS was studied. Several buffer solutions were used and the detection limits with these systems were found to be by a factor of 1–2 lower than in the conventional electrolytic cold vapor generation system. Comparing with the traditional inorganic acid, phosphate buffer solution (PBS) increased the signal intensity of Hg vapor from electrolytic generation on Pt cathode and reduced the impact of cathode erosion on the stability of signal intensity. Moreover, buffer solution has better interference tolerance. The effects of the electrolytic conditions and interference ions on the ECVG have been studied. Under optimized conditions and with PBS as catholyte the detection limit for Hg was found to be 0.27 ng L−1. The relative standard deviation was 2.8% for 11 consecutive measurements of 1 μg L−1 Hg. This method has been applied in the determination of inorganic Hg in Yangtze River water. 相似文献
29.
Rabindra N. Roy Lakshmi N. Roy Jason G. Grant Mason P. Cummins Bennett J. Tabor III Sarah J. Richards Curtis A. Himes Bret R. Lively Penny L. Blackwell Ashley N. Simon 《Journal of solution chemistry》2002,31(11):861-872
The values of the second dissociation constant, pK
2, for the dissociation of the NH+ charge center of the zwitterionic buffer compounds 4-(N-morpholino)butanesulfonic acid (MOBS), and N-(2-hydroxyethyl)piperazine-N-4-butanesulfonic acid (HEPBS) have been determined from 5 to 55°C, including, 37°C at intervals of 5°C. The electromotive-force (emf) measurements have been made utilizing hydrogen electrodes and silver–silver chloride electrodes. The value of pK
2 for MOBS was found to be 7.702 ± 0.0005, and 8.284 ± 0.0004 for HEPBS, at 25°C, respectively. The related thermodynamic quantities, G
o, H
o, S
o, and C
p
o for the dissociation processes of MOBS and HEPBS have been derived from the temperature coefficients of pK
2. Both the MOBS and HEPBS buffer materials are useful as primary pH standards for the control of pH 7.3 to 8.6 in the region close to that of physiological fluids. 相似文献
30.
乙酰化淀粉/DL-丙交酯接枝共聚物的合成及降解性能研究 总被引:7,自引:0,他引:7
用醋酸乙烯酯和玉米淀粉反应制备出了不同取代度乙酰化淀粉,再用乙酰化淀粉同DL-丙交酯接枝共聚合成乙酰化淀粉/DL-丙交酯接枝共聚物。研究了原料配比,淀粉取代度对接枝反应单体转化率(C%),接枝率(G%)接枝效率(GE%)和接枝支链数均分子量(Mn)的影响,结果表明在给定的试验条件下接枝共聚反应的C%,G%,GE%和Mn可分别达到40%,225%,80%和1.4万。接枝共聚物在磷酸缓冲溶液和户外土壤掩埋降解实验表明,在160天内样品失重率分别为71%和60%,表明合成的乙酰化淀粉/DL-丙交酯接枝共聚物具有很好的降解性能。 相似文献