Determination,residue and risk assessment of trifloxystrobin,trifloxystrobin acid and tebuconazole in Chinese rice consumption

A simple and rapid analytical method for the detection of trifloxystrobin, trifloxystrobin acid and tebuconazole in soil, brown rice, paddy plants and rice hulls was established and validated by liquid chromatography with tandem mass spectrometry. Acceptable linearity (R² > 0.99), accuracy (average recoveries of 74.3–108.5%) and precision (intra- and inter-day relative standard deviations of 0.9–8.8%) were obtained using the developed determination approach. In the field trial, the half-lives of trifloxystrobin and tebuconazole in paddy plants were 5.7–8.3 days in three locations throughout China, and the terminal residue concentrations of trifloxystrobin and tebuconazole were <100 and 500 μg/kg (maximum residue limits set by China), respectively, at harvest, which indicated that, based on the recommended application procedure, trifloxystrobin and tebuconazole are safe for use on rice. The risk assessment results demonstrated that, owing to risk quotient values of both fungicides being <100%, the potential risk of trifloxystrobin and tebuconazole on rice was acceptable for Chinese consumers. These data could provide supporting information for the proper use and safety evaluation of trifloxystrobin and tebuconazole in rice.     

In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).     

Oxidative Epigallocatechin Gallate Coating on Polymeric Substrates for Bone Tissue Regeneration

Plant derived flavonoids have not been well explored in tissue engineering applications due to difficulties in efficient formulations with biomaterials for controlled presentation. Here, the authors report that surface coating of epigallocatechin gallate (EGCG) on polymeric substrates including poly (L‐lactic acid) (PLLA) nanofibers can be performed via oxidative polymerization of EGCG in the presence of cations, enabling regulation of biological functions of multiple cell types implicated in bone regeneration. EGCG coating on the PLLA nanofiber promotes osteogenic differentiation of adipose‐derived stem cells (ADSCs) and is potent to suppress adipogenesis of ADSCs while significantly reduces osteoclastic maturation of murine macrophages. Moreover, EGCG coating serves as a protective layer for ADSCs against oxidative stress caused by hydrogen peroxide. Finally, the in vivo implantation of EGCG‐coated nanofibers into a mouse calvarial defect model significantly promotes the bone regeneration (61.52 ± 28.10%) as compared to defect (17.48 ± 11.07%). Collectively, the results suggest that EGCG coating is a simple bioinspired surface modification of polymeric biomaterials and importantly can thus serve as a promising interface for tuning activities of multiple cell types associated with bone fracture healing.     

健康住宅建设技术要点分析

通过对健康住宅建设技术要点的分析介绍，阐述了它对我国人居环境、健康的影响，以及它的重要性和对生态环境可持续发展的影响。并结合国内外健康住宅发展状况，提出了我国建设健康住宅应注意采取的技术措施及评价指标。     

期权理论在高新企业价值评估中的应用

分析了高新技术企业特点，说明了传统的企业价值评估方法不能应用于对高新技术企业的评估。如果把高新技术企业投资看成是为了获得一个未来取得现金流的机会，高新技术企业具有期权性质，就可以用期权理论及Black-Scholes模型评估高新企业的价值。     

祁连山东段冲积扇的发育时代及其成因

第四纪期间祁连山东段山麓地带广泛发育了多级冲积扇,它们是研究区域构造抬升与气候变化最为直接的载体.本文主要通过ESR,IRSL以及14C等绝对测年手段对该区冲积扇的形成年代进行研究,结果表明祁连山东段北麓最高级冲积扇形成于0.85 MaBP,而毛毛山南麓的最高级冲积扇形成于0.43 MaBP.随后各地又相继发育了0.25,0.16,0.06,0.01 MaBP等多级冲洪积台地.根据主要冲积扇的形成年代及其与区域构造气候事件的对比,认为它们是构造运动与气候变化共同作用的产物.     

嫖宿幼女罪构成要件探讨

司法解释构成嫖宿幼女罪必须要具备明知要件,这种解释违背了立法本意,不利于实际司法操作,不利于对此种犯罪行为的打击.     

IFS-BN结合的辐射源威胁评估方法

针对辐射源威胁评估实时性、复杂性和信息不确定性的问题,将直觉模糊集与贝叶斯网络相结合,提出一种基于IFS-BN的辐射源威胁评估方法。IFS-BN算法通过直觉模糊集刻画贝叶斯网络的节点信息,充分描述节点信息的确定性,不确定性以及犹豫度。同时,将直觉模糊集求得的各辐射源先验概率代入贝叶斯网络模型之中,得出后验概率进行威胁等级排序,并在此基础上建立了相应的评估指标体系。仿真分析表明,算法具有较好的可行性与有效性。与经典贝叶斯网络评估方法相比,IFS-BN算法的结果更加客观合理。     

具有急慢性阶段的MSIS流行病模型阈值和稳定性结果

系统研究了具有急性和慢性两个阶段的MSIS流行病模型.由两节构成,第1节建立和研究了具有急慢性阶段的MSIS流行病模型;第2节在第1节的基础上建立和研究了具有慢性病病程的MSIS流行病模型.第1节的模型是四个常微分方程构成的方程组.第2节的模型既含有常微分方程,又含有偏微分方程.运用微分方程和积分方程中的理论和方法,得到了这两个模型再生数R0的表达式.证明了当R0<1时,无病平衡态是全局渐近稳定性,给出了各模型地方病平衡态的存在性和稳定性条件.