电聚合吡咯膜修饰的葡萄糖氧化酶电极

自制的铂丝电极经铂化处理后,将葡萄糖氧化酶与单体吡咯用循环伏安法共聚合到电极上,铂化后电极表面积的增大增加了固定的酶量,同时也增大了电极对Ｈ２Ｏ２的催化能力,使电极对葡萄糖的响应电流增大到７．５ｍＡ／（ｍｏｌ·Ｌ＾－１）,还测试了聚合液中酶浓度、ｐＨ值和聚合时间等因素对电极特性的影响。     

再生丝心蛋白和羧甲基纤维素复合膜的特性及其在有机相葡萄糖生物传感器中的应用

用再生丝心蛋白和羧甲基纤维素构成的复合膜作为固定葡萄糖氧化酶的基质,并用红外光谱测试了该复合膜的结构,用扫描电子显微镜观察了其形态．用上述复合膜材料固定葡萄糖氧化酶和羧酸二茂铁（或７,７,８,８－四氰代二甲基苯醌,ＴＣＮＱ）制成的葡萄糖生物传感器具有较好的灵敏度与稳定性     

葡萄糖氧化酶及过氧化氢酶的生物合成

研究了黑曲霉细胞的生长及其葡萄糖氧化酶、过氧化氢酶的生物合成．ＣａＣＯ３是２种酶的产酶诱导因子,其产酶模式都是延续合成型,最大比生长速度μｍａｘ、菌体得率Ｙ（Ｘ／Ｓ）、葡萄糖氧化酶及过氧化氢酶合成得率Ｙ（ＧＯＤ／Ｓ）、Ｙ（ＣＡＴ／Ｓ）分别为０．０９７ｈ－１、０．０７５ｇ．ｇ－１、２９．３４μｍｏｌ·ｍｉｎ－１·ｇ－１、３８９．１７μｍｏｌ·ｍｉｎ－１·ｇ－１．也研究了各种物质对葡萄糖氧化酶及过氧化氢酶活力的影响,金属离子对葡萄糖氧化酶有抑制,阴离子对葡萄糖氧化酶和过氧化氢酶有抑制,还原性小分子化合物是葡萄糖氧化酶的激活剂     

鸭血烯酸对艾氏癌生长的抑制作用

鸭血烯酸是由鸭血清中提取的不饱和脂肪酸．在体外实验中具有使艾氏腹水癌细胞膨胀失活的作用．用于治疗艾氏癌患鼠,可抑制其腹水癌和实体瘤的生长．与环磷酰胺（CPA）或氟脲嘧啶（SFU）相比,鸭血烯酸的毒性较低,疗效较高,约20％～40％被治愈的患鼠,可长期存活．     

Ferrocene-Nafion修饰厚膜碳糊电极的葡萄糖传感器研究

以基于丝网印刷技术制作的厚膜碳糊电极为基底电机,用二茂铁为介体、Nafion修饰厚膜碳糊电极制备了葡萄糖传感器．Nafion涂于电极表面上形成的膜具有强的附着力,防止了二茂铁及酶的流失,电极稳定性提高．并且由于低的工作电位（＋025vsSCE）和Nafion膜的阳离子交换作用,基本上消除了电活性物质（抗坏血酸、尿酸）的干扰,具有防污能力．该酶传感器的检测上限可达18mmol／L,响应时间小于60s.     

针刺对胸内肿瘤化学疗法中自由基等指标的影响

恶性肿瘤的化学疗法会产生自由基升高和白细胞数目降低等副作用,临床表现恶心、呕吐、厌食、疲乏等症状。 本文应用针刺“足三里”、“阴陵泉”、“内关”等穴位配合化学疗法,结果血中脂质过氧化物(LPO)降低,白细胞增多、消化道症状和疲乏感降低,这说明针刺疗法可作为化学疗法的重要支持手段。 针刺降低自由基的原理估计是激发了体内降解酶的作用。     

葡萄糖氧化酶电极的制备

葡萄糖氧化酶电极的制备于秀娟，周定，郭京华（哈尔滨工业大学应用化学系哈尔滨１５０００６）关键词葡萄糖氢化酶，酶电极，制备酶与电极的连接即酶膜的形成是制备酶电极的关键步骤之一，有许多种方式［１～９］．葡萄糖氧化酶（ＧＯＤ）电极是研究较早和报道较多的一类...     

Comparison of different strains of the yeastYarrowia lipolytica for citric acid production from glucose hydrol

Four commercial strains and two mutants of the yeast species Yarrowia lipolytica were screened using batch fermentation. Strain Y. lipolytica A-101-1.14 (induced with UV irradiation) was found to be the most suitable for citric acid production from glucose hydrol (39.9% glucose and 2.1% other sugars), a byproduct of glucose production from potato starch. The specific rate of total citric and isocitric acid production was 0.138 g/g.h, the yield on consumed glucose 0.93 g/g, and the productivity achieved was as high as 1.25 g/L.h. All of the tested yeast strains were able to utilize only the glucose from the glucose hydrol medium. Thus, some residual higher oligosaccharides remained in the process effluent.     

Interaction of adenine and thymine with aqueous sugar solutions

The solubility of the nucleic acid bases, adenine and thymine, in aqueous erythritol, xylose, glucose, and sucrose solutions has been studied. The solubility of adenine increases linearly with glucose and sucrose concentration, whereas with the other reagents a nonlinear increase is observed. Below 1.5M reagent concentration, the solubility of adenine increases in the order erythritol < robose, xylose < glucose < sucrose. The solubility of thymine in these solutions, on the other hand, decreases, increases, or does not change depending upon the reagent. The effect of temperature on the solubility of adenine and thymine in sugar solution indicates that the transfer of these molecules from water to sugar solution is exothermic.Presented in part at the VIIth All-India Symposium in Biophysics held at Visva Bharati University during October 1976.