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81.
为了探讨生物刺激、生物强化及其联合体系对石油污染物的去除能力,选择最佳的修复策略,更好地发挥微生物修复的优势.以一株蜡状芽孢杆菌石油降解菌为例,以有机营养物、无机营养物和混合营养物为底物,进行生物刺激、生物强化试验及联合降解试验.结果显示,不同修复方式在不同时段的降解效果不同,生物刺激方式在第5天时混合营养物降解效果最好,降解率达到40.98%,在10 d不同营养物的降解率存在明显的差距,而在处理20 d后,降解率基本一致,达到80%.生物强化的处理结果中,以混合营养物为底物时,表现出了优异的降解效果,降解率达到90.23%.研究表明:不同时期不同的修复策略有表现出了对原油的不同去除能力,也为实践中选择合理的修复方式提供科学的理论指导. 相似文献
82.
83.
Dr. Chetan Kumar Arya Swati Yadav Dr. Jonathan Fine Dr. Ana Casanal Dr. Gaurav Chopra Prof. Dr. Gurunath Ramanathan Dr. Kutti R. Vinothkumar Prof. Dr. Ramaswamy Subramanian 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(39):17109-17114
N,N-dimethyl formamide (DMF) is an extensively used organic solvent but is also a potent pollutant. Certain bacterial species from genera such as Paracoccus, Pseudomonas, and Alcaligenes have evolved to use DMF as a sole carbon and nitrogen source for growth via degradation by a dimethylformamidase (DMFase). We show that DMFase from Paracoccus sp. strain DMF is a halophilic and thermostable enzyme comprising a multimeric complex of the α2β2 or (α2β2)2 type. One of the three domains of the large subunit and the small subunit are hitherto undescribed protein folds of unknown evolutionary origin. The active site consists of a mononuclear iron coordinated by two Tyr side-chain phenolates and one carboxylate from Glu. The Fe3+ ion in the active site catalyzes the hydrolytic cleavage of the amide bond in DMF. Kinetic characterization reveals that the enzyme shows cooperativity between subunits, and mutagenesis and structural data provide clues to the catalytic mechanism. 相似文献
84.
新型生物反应墙原位修复石油烃污染地下水 总被引:3,自引:0,他引:3
以功能微生物、泥炭和粗砂为填充介质设计新型生物反应墙,研究了反应墙对地下水中石油烃污染物的修复效果与机理。结果表明:运行期内生物反应墙修复效果良好,苯系物、萘系物及菲去除率为83.6%~99.85%,其中71.23%~99.71%在墙体前半部分被去除。泥炭介质和功能微生物能够稳定发挥对污染物的吸附与降解功能,32.63%~77.98%的BTEX和97.14%~99.81%的目标PAHs被泥炭吸附去除;18.96%~50.98%的BTEX和已吸附于泥炭上的大部分石油烃污染物均显示被生物降解。微生物对污染物的降解可有效延长泥炭的吸附寿命,泥炭对功能微生物的营养供给可使反应墙内长期保持较高的功能微生物数量,每克干介质约含有3.46×106~6.16×109个。因此新型生物反应墙原位修复石油烃污染地下水是可行的。 相似文献
85.
86.
污染土壤原位生物修复理论模型 总被引:2,自引:0,他引:2
本文针对污染土壤原位生物修复技术,建立了土壤中气-液两相流动、污染物扩散与生物降解的理论模型,并进行了计算和分析,讨论了渗透廊的加入对液体饱和度的分布、液膜厚度分布以及污染物浓度分布的影响。结果表明:调节渗透廊内液面高度和渗透廊底面厚度,可以控制土壤中液体饱和度及液膜厚度的分布,且对污染物降解效率具有明显的影响。 相似文献
87.
Luiz Carlos Martins Das Neves Tábata Taemi Miazaki Ohara Miyamura Dante Augusto Moraes Thereza Christina Vessoni Penna Attilio Converti 《Applied biochemistry and biotechnology》2006,129(1-3):130-152
Industrial effluents from the pharmaceutical industry often contain high concentrations of phenolic compounds. The presence
of “anthropogenic” organic compounds in the environment is a serious problem for human health; therefore, it merits special
attention by the competent public agencies. Different methods have been proposed in the last two decades for the treatment
of this kind of industrial residues, the most important of which are those utilizing absorption columns, vaporization and
extraction, and biotechnological methods. Biofiltration is a method for the removal of contaminants present in liquid or gaseous
effluents by the use of aerobic microorganisms, which are immobilized on solid or porous supports. Although several bacteria
can utilize aromatic compounds as carbon and energy source, only a few of them are able to make this biodegradation effectively
and with satisfactory rate. For this reason, more investigation is needed to ensure an efficient control of process parameters
as well as to select the suited reactor configuration. The aim of this work is to provide an overview on the main aspects
of biofiltration for the treatment of different industrial effluents, with particular concern to those coming from pharmaceutical
industry and laboratories for the production of galenicals. 相似文献
88.
Modeling the Enhanced Bioremediation of Organic Contaminants in Pyrite-containing Aquifers 总被引:1,自引:0,他引:1
The bioremediation of organic contaminants in the subsurface is strongly influenced by the existing geochemical environment.
In this study a coupled reactive transport and geochemical model is developed for the simulation of enhanced bioremediation
of organic contamination in the presence of pyrite. The two-dimensional model allows for the simulation of both kinetically
defined as well as geochemical equilibrium reactions. The model is applied to a hypothetical pyrite-containing aquifer contaminated
with petroleum hydrocarbons. Oxygen injected into the aquifer to enhance contaminant biodegradation reacts with pyrite resulting
in reduced oxygen availability, acidification of the subsurface environment and, subsequently, the inadvertent inhibition
of the microbial activity. The reactive transport and geochemical model is used to quantify these processes. The dominance
of the various chemical reactions and the sensitivity of the biodegradation on pyrite content are evaluated. Through groundwater
pH manipulation, the interference of pyrite with the intended remedial action is partially mitigated. It is shown that when
oxygen availability is a limiting factor, the optimal pH that would maximize hydrocarbon degradation may significantly differ
from the pH value that maximizes bacterial activity. 相似文献
89.
Paweł Głuszcz Katarzyna Zakrzewska Irene Wagner-Doebler Stanisław Ledakowicz 《Chemical Papers》2008,62(3):232-238
Wide industrial use of mercury led to significant mercury pollution of the environment. It requires development of cleanup
technologies which would allow treating large volumes of mercury contaminated water in a cost effective and environmentally
friendly way. A novel bio-technology, developed from laboratory to industrial scale in Germany at HZI (former GBF), is based
on enzymatic reduction of highly toxic Hg(II) to water-insoluble and relatively non-toxic Hg(0) using live mercury resistant
bacteria immobilized on a porous carrier material in a fixed-bed bioreactor. Improvement of the original method was based
on the use of activated carbon as a carrier for microorganisms and an adsorbent for mercury. Such integration of the process
should increase the technology efficiency. In order to compare different carrier materials, activated carbon and pumice stones
were used. The strain Pseudomonas putida was immobilized in bioreactors continuously fed with solutions of HgCl2 enriched with nutrients. Simultaneously, experiments in two more reactors were run in the absence of microorganisms to investigate
the influence of nutrients on the adsorption process. In the bioreactor with activated carbon, the outlet mercury concentration
was approximately 50 % of that supplied with pumice. It may be concluded that the use of activated carbon in a fixed-bed bioreactor
enables improvement of the technology by process integration.
Presented at the 34th International Conference of the Slovak Society of Chemical Engineering, Tatranské Matliare, 21–25 May
2007. 相似文献
90.
Effects of Cyclodextrins on Dodecane Biodegradation 总被引:1,自引:0,他引:1
Silvia Steffan Patrizia Tantucci Laura Bardi Mario Marzona 《Journal of inclusion phenomena and macrocyclic chemistry》2002,44(1-4):407-411
Bioremediation of non-chlorinated hydrocarbon-polluted soils is mainly affectedby low bioavailability, due to hydrophobicity of these xenobiotics. In fact, severalmicroorganisms can use hydrocarbons as energy and carbon sources, but theirdegradative activity takes place into the aqueous phase of the soil, where just tracesof hydrocarbons are found because of their low water solubility. So, natural attenuationusually occurs in hydrocarbon-polluted soils, but this process is very slow. It has alreadybeen demonstrated that cyclodextrins increase hydrocarbon solubility and bioavailabilityand accelerate their biodegradation. In this work it was investigated if their efficacy onbiodegradation of a model hydrocarbon (dodecane) is affected by the kind (,-, - and hydroxypropyl--cyclodextrin) and the concentrationof cyclodextrin and by environmental factors such as temperature and composition ofthe microbial indigenous population. The results obtained show that all the testedfactors influence the biodegradation kinetics. The best results were obtained with-cyclodextrin at a concentration near to its water solubility limit; moreover,biodegradation rate increases with temperature and different microbial strains showdifferent degradative activity and metabolic behaviour. 相似文献