取代苯甲酸的QSBR研究

定义新的原子点价（gi）并建构生物降解性连接指数（^mM）．其中的M、^1M与取代苯甲酸的生物降解速度常数（1gk）具有良好的相关性：-1gl=-0．7832＋0．2637^0M—0．0716^1M，R^2=0．9889，计算值与实验值基本吻合．     

三聚氰胺快速生物降解研究

研究三聚氰胺在特定温度条件下的快速生物降解。将受试物三聚氰胺和参比物定量加入一定体积的已接种少量微生物的培养基中,三聚氰胺作为唯一的有机碳源,在黑暗恒温条件下保持受试物溶液完全充满密闭瓶。根据在28d中溶解氧的消耗来计算降解率。受试物三聚氰胺生物降解时微生物菌落消耗的氧量经平行试验的接种空白对照校正后,以ThOD的百分数表示降解率。参比苯甲酸钠28d降解率为71.4%,而三聚氰胺降解率为6.73%。判定三聚氰胺属于不易快速生物降解物质。     

生物可降解PLA-PEG共聚物微球的制备及表征

首先利用开环聚合的方法 ,制备了聚乳酸 -聚乙二醇嵌段共聚物 .然后以此为基材 ,采用乳化溶剂蒸发法 ,制备了聚乳酸 -聚乙二醇嵌段共聚物微球 ,分别研究了聚合温度、聚合时间和聚乙二醇的分子量等对共聚物的特性粘数 [η]的影响以及稳定剂、乳化剂、共聚物 [η]、搅拌速度及油 /水比对共聚物微球的影响 .     

淀粉-丙烯酸接枝共聚物的生物降解研究

采用真菌生长法和土壤掩埋法对淀粉－丙烯酸接枝共聚高吸水树脂的生物降解性能进行了研究，运用表现观察法、重量测定法对试样的降解过程进行了测试，并对原有的测试方法作了改进，试验结果表明，淀粉－丙烯酸接枝共聚物具有较好的生物降解性能。     

Tetrahydroisoquinoline N-methyltransferase from Methylotenera Is an Essential Enzyme for the Biodegradation of Berberine in Soil Water

Berberine (BBR), a Chinese herbal medicine used in intestinal infection, has been applied as a botanical pesticide in the prevention of fungal disease in recent years. However, its degradation in the environment remains poorly understood. Here, we investigated BBR’s degradation in soil water from different sources accompanied by its effect on bacterial diversity. Our results indicated that BBR was only degraded in soil water, while it was stable in tap water, river water and aquaculture water. Bacterial amplicon results of these samples suggested that the degradation of BBR was closely related to the enrichment of Methylotenera. To reveal this special relationship, we used bioinformatics tools to make alignments between the whole genome of Methylotenera and the pathway of BBR’s degradation. An ortholog of Tetrahydroisoquinoline N-methyltransferase from plant was discovered only in Methylotenera that catalyzed a crucial step in BBR’s degradation pathway. In summary, our work indicated that Methylotenera was an essential bacterial genus in the degradation of BBR in the environment because of its Tetrahydroisoquinoline N-methyltransferase. This study provided new insights into BBR’s degradation in the environment, laying foundations for its application as a botanical pesticide.