丁二酸酐修饰啤酒废酵母菌吸附阳离子染料的研究

以戊二醛为交联剂,用丁二酸酐修饰啤酒废酵母菌表面制成新的生物吸附剂(简称修饰酵母菌).研究发现,修饰酵母菌吸附阳离子染料的性能明显优于未修饰酵母菌.实验结果表明,在室温、溶液的pH=4.0～8.0、吸附时间1 h的条件下,修饰酵母菌能有效吸附亚甲基蓝和中性红.吸附模式以Langmuir单分子层化学吸附为主.修饰酵母菌对...     

非抑制电导阳离子色谱法测定啤酒中的阳离子和胺类化合物

建立了以甲基磺酸(MSA)和乙腈混合淋洗,离子交换色谱分离,非抑制电导检测,分析分离啤酒中阳离子和胺类化合物的方法.色谱条件经优化,在25 min内分离出啤酒中的7种阳离子和胺类化合物,方法的回收率和相对标准偏差分别为94.31%～109.9%和0.10%～1.2%.     

催化动力学光度法测定啤酒中的乙醇

基于乙醇对过硫酸钾与亮绿间的氧化褪色反应的负催化作用 ,建立了一种定量乙醇的新方法 .该法的线性范围为 4.0× 10 -5～ 2 .0× 10 -3 mol/L ;检出限为 7.6× 10 -6mol/L .用于实际啤酒样品中乙醇含量的测定 ,结果良好     

松针和啤酒相互作用吸收光谱研究

采用光谱分析技术,研究了松针萃取液及其与啤酒相互作用的吸收光谱,结果表明,二者大部分波段的吸光度是相似的,松针萃取液和啤酒相互作用吸收光谱的吸光度与松针萃取液的吸光度相比明显增强,且峰值位置产生了一定的位移．但300—375nm段的吸光度有较明显的差异,表现出吸收光谱在吸光度和峰值位置上的差异．     

固相萃取-高效液相色谱法同时测定啤酒中的4种异构化α-酸

建立了固相萃取-高效液相色谱同时测定啤酒中4种异构化α-酸的方法。采用Sep-Pak C18萃取柱,系统研究了啤酒中异构化α-酸的最佳固相萃取条件。选择以2 mL酸化甲醇为洗脱溶剂,萃取前调啤酒样品的pH至2.5。该方法准确可靠,重现性好,4种异构化α-酸的回收率为90.6%～96.4%,相对标准偏差小于4%。异α-酸、二氢异α-酸、四氢异α-酸和六氢异α-酸的最低检测限依次为0.14,0.36,0.33和0.53 mg/L。     

用啤酒酵母试制代可可粉及可可风味饮料的研究

用啤酒厂酵母泥为主要原料，研究以小试规模生产代可可粉的生产工艺，以及用代可可粉调制可可风味饮料的配方。重点研究用化学试剂组合脱酵母臭的方法，研究制出与天然可可粉质地、大小、色泽和风味相似的代可可粉。     

蔗汁生产啤酒发酵特性的研究

论述了在100L啤酒生产设备上,分别用大米与蔗汁为辅料生产啤酒的对照试验,通过考察两种辅料所得麦汁的主要成分,对两种麦汁的外观发酵度、可发酵浸出物、酵母利用还原糖和α-氨基氮的情况、酒精生成量及双乙酰等发酵参数进行研究.结果表明,酵母在添加蔗汁辅料的麦汁中能正常发酵,所得啤酒的各项理化指标均符合国家标准GB4927.     

啤酒发酵过程的建模仿真与控制

针对啤酒发酵过程的特点,建立了数学模型,设计了多变量温度控制系统,并提出一种面向工业过程的多变量时滞系统仿真方法。该控制系统已在自行研制的AUTO－2000集散控制系统中实现,并成功用于安徽圣泉啤酒厂的啤酒发酵温度控制,现场运行情况表明,系统可靠性高,适应性强,有效提高了啤酒的质量和产量,取得了每年1千多万元的经济效益。     

Expedient Synthesis of Lupulones and Their Derivatization to 2,8-7H-Dihydrochromen-7-ones

A convenient and improved method for the synthesis of beta acids or lupulones, which are known to possess e. g. anti-cancer, anti-inflammatory, anti-oxidative and antimicrobial activity, has been developed successfully. Further derivatization of these complex structures to the corresponding dihydrochromen-7-ones, including the natural product machuone, was realized to simplify their analysis and to confirm their molecular structure. In addition to practical and safe laboratory procedures, the advantages associated with this new approach involve the use of water as a solvent and the direct crystallization of lupunones from acetonitrile, rendering our strategy more efficient and benign as compared to available methods.     

Efficient entrapment of inorganic Se species in water and beer samples with functional groups-rich monolith-based adsorbent

An efficient multiple fibers solid-phase microextraction method based on porous monolith was established for Se(IV) and Se(VI) analysis. Poly(4-vinylphenylboronic acid/styrene-co-ethylene dimethacrylate/divinylbenzene) monolith was fabricated and employed as the extraction phase for efficient entrapment of Se(IV) complexed with o-phenylenediamine, followed by elution with a methanol/formic acid (99/1.0, v/v) mixture and quantification by high-performance liquid chromatography with diode array detector. The Se(VI) species was measured by the difference between total inorganic Se and Se(IV) after pre-reduction. Different characterization techniques were employed to inspect the structure and morphology of prepared adsorbent. A series of key extraction factors were optimized so as to achieve the expected extraction performance. Under the optimized separation and capture parameters, the linear range and limit of detection for Se(IV) in water sample were 0.050–200 and 0.013 μg/L, respectively. For beer sample, the corresponding values were 0.010–300 and 0.032 μg/L. The developed microextraction approach was successfully utilized to detect trace Se(IV) and Se(VI) in environmental water and beer samples with satisfactory fortified recovery and repeatability. Results well reveal the attractive merits of the established method in the analysis of Se species, including simple preparation of adsorbent, convenient extraction procedure, good sensitivity, high cost-effectiveness, and eco-friendliness.