Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

A Novel Digital Audio Watermarking Scheme in the Wavelet Domain

We present a novel quantization-based digital audio watermarking scheme in wavelet domain. By quantizing a host audio‘s wavelet coefficients (Integer Lifting Wavelet Transform ) and utilizing the characteristics of human auditory system ( HAS), the gray image is embedded using our watermarking method. Experimental results show that the proposed watermarking scheme is inaudible and robust against various signal processing such as noising adding, lossy compression, low pass filtering, re-sampling, and re-quantifying.     

基于稳态视觉诱发电位的脑电控制上肢康复机器人

针对现阶段基于脑机接口(brain-computer interface,BCI)的康复机器人存在多目标分类时间长、识别准确率仍有待提升的问题,设计了一种由脑电信号控制的上肢康复机器人,对脑电信号中的稳态视觉诱发电位(steady-state visual evoked po-tential,SSVEP)分类,进而判断...     

ABR-BAF组合工艺处理大蒜切片废水

研究了厌氧折流板反应器(anaerobic baffled reactor, ABR)与曝气生物滤池(biological aerated filter,BAF)的组合工艺处理大蒜切片废水的运行特点。结果表明：ABR的最佳水力停留时间（HRT）为24?h,有机负荷(COD)小于6?kg/m3·d为宜,COD的平均去除率可达87%;BAF的最佳HRT为16?h,最佳气水比为10∶1,进水有机负荷(COD)宜保持在1.1～1.4?kg/m3·d,COD的平均去除率为82.0%。ABR BAF组合工艺COD总去除率保持在98.4%～98.7%之间,出水的COD质量浓度为79～94?mg/L,出水水质满足GB8978 1996《污水综合排放标准》一级标准。     

基于多重响应的大跨度空间网格结构重要构件评估方法

为统一大跨度空间网格结构在进行连续性倒塌分析时移除构件的判别方法.基于稳定与强度失效模式,给出大跨度空间网格结构重要构件初选范围的选取方法.在此基础上提出了基于初选范围的多重响应分析法,并将该方法通过算例进行验证后应用于世界大学生运动会主体育场中,得到此类折面空间网格结构的重要构件分布.该分析结果与实际工程的设计结果吻合较好.研究表明:基于初选范围的多重响应分析法简单实用,能够快速全面地评估大跨度空间网格结构中构件的重要性,弥补了现有方法只考虑单一结构响应的不足,解决了目前连续性倒塌分析中初始失效构件判别没有统一标准的缺陷,使备用荷载路径法得到了更广泛的应用.     

延迟容忍网络中基于不同转发行为路由策略研究

在延迟容忍网络(DTN)中,大多数已有的路由算法在整个数据传输过程中采用单一的数据转发行为。本文提出了一种基于人类移动模式设计不同数据转发行为的路由策略。该策略将个体移动划分为两种状态：一种是Normal状态,另外一种为Small状态,在Normal状态时,采用三因素标准来严格选取中继节点,在Small状态时,采用小范围洪泛来降低端到端延迟。仿真结果表明该策略与传统策略相比可有效提高路由效率。     

爆炸荷载下FRP加固双向板动力响应数值模拟

为了研究外贴FRP加固钢筋混凝土双向板的抗爆性能,采用有限元软件ANSYS/LS-DYNA对其在爆炸荷载下的动力响应进行模拟,比较分析了不同CFRP粘贴层数、粘贴密度和不同FRP材料对其抗爆性能的影响.结果表明,相同爆炸荷载下,随着CFRP粘贴层数和粘贴密度的增加,加固板抗爆性能不断增强,但考虑经济性和延性,CFRP粘...     

土工格栅加固路桥过渡段的动测试分析

在秦沈铁路客运专线乙试验工点现场试验的基础上,对土工格栅加固后的路桥过渡段基床动响应特性及其影响因素进行了分析和研究.研究结果表明:随着行车速度增大,过渡段基床动应力增量为正;但当行车速度超过临界速度(220～230 km/h)后,基床动应力不再增加,反而减小;基床动应力的变化特征与其在基床中的位置密切相关,沿线路纵向水平方向上,动应力随距桥台背距离的增大而呈减小趋势;沿深度方向上,动应力随深度的增加而呈衰减趋势;过渡段沉降差的存在导致基床动应力增大,严格控制过渡段沉降差是高速铁路设计和施工的关键.     

Frequency invariant beamforming via jointly optimizing spatial and frequency responses

Inbroadbandarraysignalprocessing ,thespec tralcharacteristicsofthetargetaremostlyusedintargetdetectionandtargetclassification .Sincetheradiatednoiseofthetargetgenerallyspreadsoverawidefrequencyband ,distortioninthepowerspec trummightappearifthebeamformerresponsevarieswithfrequency .Theso calledfrequencyinvariantbeamformer (FIB)techniqueisrequiredasaremedy .AnFIBisabeamformerinwhichtheresponseisap proximatelyconstantoversomedesignbandwidth .InRef .[1],anapproachtodesigningbroadbandbeamformer…     

具有硝化作用的异养菌脱除氨氮的性能研究

从消化污泥中筛选、分离出具有硝化作用的异养菌用于校园生活污水的处理。工艺采用厌氧折流板反应器(ABR)和序批式活性污泥反应器(SBR)。结果表明：当ABR停留时间5h，SBR曝气时间3h，投菌量50g／L，温度(28～30)℃，CODCr／NH3-N≥4．4时，其对NH3-N、CODCr、浊度去除率分别达84．1％、94．7％、93．0％。