毛细管区带电泳法测定冬虫夏草中的腺苷、腺嘌呤和尿嘧啶

建立了毛细管区带电泳法测定天然和人工冬虫夏草中腺苷、腺嘌呤和尿嘧啶含量的分析方法。采用15mmol/L硼砂＋14mmol/L磷酸二氢钠＋5％（V／V）甲醇（pH＝9.5）作为缓冲体系，在电压为18kV和检测波长为254nm的条件下，冬虫夏草提取液中的腺苷、腺嘌呤和尿嘧啶实现了基线分离。定量分析表明，3种成分的校正峰面积与质量浓度呈较好的线性关系(r≥0.9991）。考察了缓冲溶液的pH值、浓度及有机改性剂对腺苷、腺嘌呤和尿嘧啶迁移行为的影响。     

金属配合物-寡聚核苷酸定位断裂剂研究进展

核酸切割试剂与寡聚核苷酸(ODN)偶联制得的人工核酸酶能在特定位点断裂DNA或RNA,为人工核酸酶的分子设计提供了一种新方法.本文综述了金属配合物-ODN识别切割试剂的偶联方式及其与靶分子的作用机制,并指出了今后的研究方向.     

Synthesis and Characterization of Two Novel High Valent Dinuclear Complexes with a Triphenolate Ligand Bearing Functional Groups

Two novel high valent complexes [M2(III, III)L(p--OAc)2].PF6 (M=Mn, Fe; 9) were prepared, where L was the tri-anion of 2,6-bis{[(2-hydroxy-3-(morpholin-4-yl methyl)-5-tertbutyl benzyl)(pyridyl-2-methyl)amino]methyl}-4-methyl phenol which contained additional phenolic, tert-butyl and morpholin-4-yl methyl groups compared to its parent [Mn2(Ⅱ, Ⅱ)(bpmp)(μ-OAc)2]-ClO4 (10). These improvements decreased the difference between the new model and (Mn)4 cluster (OEC in nature).     

基于支持向量机的羰基化合物红外光谱研究

设计了一个4层分等级分类系统，通过支持向量机技术对羰基化合物的红外光谱成功地进行了信息分类和提取，并与人工神经网络进行比较，结果表明：支持向量机对羰基类化合物红外光谱分类效果优于人工神经网络；另外详细探讨了表征酰胺类化合物的特征光谱峰片段对识圳伯仲叔酰胺的影响，证明了酰胺的N-H伸缩振动峰对识别伯仲叔酰胺贡献最大，为构建红外光谱智能解析系统提供了定量依据。     

人工光合作用反应中心的研究进展

本文对几种人工光合作用反应中心系统,做一个简单的综述,其中包括叶绿素和细菌叶绿素二聚体,卟啉二聚体,卟啉-苯醌共价键络合物以及其他合成中心。     

Artificial red cells with crosslinked hemoglobin membranes

Artificial cells containing concentrated hemoglobin (Hb) solution were prepared by interfacial polymerization of Hb with glutaraldehyde (GA) in liquid membrane capsules (LMC). A solution containing 30% of Hb was emulsified in mineral oil as red cell-size microdroplets, and this emulsion was dispersed in an aqueous phase containing glutaraldehyde to form LMC. The LMC were semipermeable templates that held the microdroplets of Hb in suspension while GA diffused through the oil to the microdroplet surfaces. The GA crosslinked Hb at the surface of each microdroplet to form an artificial red-cell membrane encapsulating Hb solution. A water-soluble surfactant was used to eject the cells from the LMC and suspend them in saline. Several surfactants were evaluated. Cell size was controlled by agitation speed during preparation of the original emulsion. Cells of 2.52 = ±1.69 μm were prepared. The encapsulated Hb retained capacity to bind and release O₂. The cells had aP ₅₀ of 8.9 torr (1200 Pa) and a capacity of 0.55 cc O₂/g of total Hb, indicating that the crosslinked portion of the Hb did not contribute to O₂ transport.     

蛋白芯片法同时检测食品中克伦特罗与莱克多巴胺的残留量

建立了食品中克伦特罗和莱克多巴胺残留含量同时检测的微阵列蛋白芯片法。结果显示:克伦特罗的线性范围为0.07～1.2 ng/g;莱克多巴胺的线性范围为0.05～0.8 ng/g。猪肉、猪肝中上述两种物质的加标回收率为74%～132%,相对标准偏差均小于10%。将所建立的方法与HPLC-MS方法进行对比,两者检测结果一致。该方法简单、快速、通量高,可用于实际样品中克伦特罗和莱克多巴胺残留量的检测。     

Solar energy conversion from water photolysis by biological and chemical systems

The production of chemicals and fuels, or energy-rich compounds, from water by sunlight is described as a particularly attractive means for the conversion of solar energy to a valuable renewable resource. The redox properties of photoexcited molecules and the operating mechanism of light-driven systems are first considered. The mechanism of water oxidation carried out by higher plants and green algae-which is actually one of the most important biochemical reactions—as well as that of artificial photosystems, up-to-now designed trying to simulate the natural process with higher efficiency and simplicity, are likewise discussed. A number of biological and chemical light-driven systems are presented as practical ways to solar energy conversion.     

Heat capacities of concentrated multicomponent aqueous electrolyte solutions at various temperatures

The specific heat capacities of the aqueous multicomponent system NaCl +KCl+MgCl₂+CaCl₂ with ionic strength between 8.3 and 9.6 (resembling Dead Sea waters) were measured between 15°C and 45°C. The obtained data were fitted to an empirical equation as a function of concentration and temperature. The thermodynamic functions of the studied multicomponent system were found to be strongly influenced by changes in MgCl₂ concentrations. The application of Young's rule to such concentrated systems was checked at 25°C. The calculated (by Young's rule) specific heat capacitiesC _p and apparent molar heat capacities C_p, of these multicomponent electrolyte solutions were in reasonable agreement with the measured values (–0.008 J-g^–1-K^–1 and –2.6 J-mol^–1-K^–1, respectively).     

裂纹悬臂方薄板的振动辐射声场

为研究含穿透裂纹悬臂方板的振动辐射声场，根据有限元模态分析理论，提取了含人工斜向和十字形穿透裂纹的悬臂方薄板各单元的相关物理参数，同时将瑞利积分离散化，进而计算出裂纹悬臂方薄板辐射声场的空间分布，并通过实验测定了含裂纹悬臂方薄板辐射声场的功率谱。结果表明，斜向裂纹越长，二、四、六阶模态的频率降低越多，而一、三、五阶模态频率随斜向裂纹长度的增大表现为先增大再减小的规律；十字裂纹越长，各阶模态的频率降低越多。穿透裂纹的出现不仅使悬臂板的模态频率有明显的变化，而且声场在空间的分布特征也有显著的变化。