A Novel Fluorescence Detection Method For Organosilakes

Abstract

Quinones such as phenanthraquinone and anthraquinone react with organosilanes giving persistent, strongly fluorescent radicals. the reactions are photochemically initiated, rapid, and facile; detection of concentrations of silanes in the 10^?7 M range are readily achieved. the starting materials do not fluoresce making the procedure simple, selective and specific.     

大黄蒽醌类化合物电喷雾质谱研究

采用电喷雾-离子阱质谱(ESI-ITMS)法,通过一级质谱全扫描和二级质谱碰撞诱导解离技术,研究5种大黄蒽醌衍生物(大黄素、芦荟大黄素、大黄酸、大黄素甲醚、大黄酚)的质谱行为及分子结构与裂解规律间的关系,并对大黄药材中总游离蒽醌提取物进行了电喷雾质谱检测.实验结果显示,5种大黄蒽醌类化合物一级质谱负离子出峰较好,被测样品均为基峰或第二强峰,未发现聚合体离子及加合离子产生,二级质谱各碎片离子归属明确,特征性强.实验结果可应用于大黄蒽醌类化合物的结构分析及进一步的代谢产物研究,并为大黄药材有效成分的鉴定提供了一种快速,灵敏的检测方法.     

反相高效液相色谱测定大黄及其制剂中蒽醌类成分

建立1个同时测定大黄及其制剂中的芦荟大黄素、大黄酸和大黄素含量的反相高效液相色谱法.采用Waters Spherisorb ODS2(5μm,Φ4.6 mm×250 mm)色谱柱,以体积比为85∶15的甲醇-0.1%磷酸溶液为流动相,流速为1.5!mL/min,柱温为40℃,检测波长为430 nm.在上述色谱条件下,芦荟大黄素、大黄酸和大黄素在10 min内得到了有效的分离和检测.方法简便、快速、灵敏、线性范围宽,可用于大黄及其制剂中芦荟大黄素、大黄酸和大黄素的定量测定.     

铕对决明发根生长及有效成分生成的作用

以决明发根为材料,在培养基中附加０．００１～１０ｍｇ．Ｌ＾－１Ｅｕ＾３＋（ＥｕＣ１３）,测量发根的鲜重和干重,用高效液相色谱法测定游离蒽醌化合物含量。发有低剂量（０．００１,０．０１,１．０ｍｇ．Ｌ＾－１）Ｅｕ＾３＋抑制生长,０．００１ｍｇ．Ｌ＾－１Ｅｕ＾３＋抑制作用最强,干重比对照降低２２％;较低剂量（１０ｍｇ．Ｌ＾－１）Ｅｕ＾３＋处理的含量高于对照２６＾,高于Ｅｕ＾３＋１．０ｍｇ．Ｌ＾－１处理     

Simultaneous quantification of five anthraquinones in rat plasma by high-performance liquid chromatography with fluorescence detection

A sensitive high-performance liquid chromatographic method with fluorescence detection (excitation 435 and emission 515 nm) was established and validated for quantification of five anthraquinones (aloe-emodin, rhein, emodin, chrysophanol and physcion) in rat plasma. Following a single-step liquid-liquid extraction, the analytes and internal standard (1,8-dihydroxyanthraquinone) were separated on a reversed-phase C(18) column with water-phosphoric acid-methanol as mobile phase at a flow rate of 1 mL/min. The linear ranges of the calibration curves were 6.5-1300 ng/mL for aloe-emodin, 20-4000 ng/mL for rhein, 40-8000 ng/mL for emodin, 15-3000 ng/mL for chrysophanol and 13-2600 ng/mL for physcion. The lower limit of quantification was 6.5 ng/mL for aloe-emodin, 20 ng/mL for rhein, 40 ng/mL for emodin, 15 ng/mL for chrysophanol and 13 ng/mL for physcion. The mean accuracy was 94.3-105.1% for aloe-emodin, 90.3-108.8% for rhein, 92.6-106.7% for emodin, 95.8-103.8% for chrysophanol and 98.7-101.2% for physcion. The within-batch and between-batch precisions were < or = 5.5% and < or = 13.4%, respectively. This method is suitable for determining the five anthraquinones in plasma simultaneously and thus investigating the pharmacokinetics of anthraquinones from Xiexin decoction in rats.     

虎杖灌胃大鼠血尿中蒽醌类代谢物的LC-MS分析

通过检测虎杖提取物灌胃后大鼠血浆及尿液中的代谢产物, 建立一种用液相色谱 电喷雾串联质谱（LC-MS）分离鉴定复杂样品中主要蒽醌类代谢物的方法. 液相色谱条件： 色谱柱为Agilent XDB-C18（4.6 mm×250 mm, 5 μm）, 流动相为水 甲醇梯度洗脱, 柱温为25 ℃. 质谱条件： ESI离子源, 毛细管电压3.5 kV, 负
离子检出模式. 结果表明: 在大鼠灌胃虎杖提取物后的血浆样品中检测出大黄素、 大黄酚和大黄素甲醚3种代谢产物; 尿液中检测出大黄素、 大黄素葡萄糖醛酸酯、 芦荟大黄素硫酸酯和芦荟大黄素葡萄糖醛酸酯4种代谢产物.     

Effects of Anthraquinones on Immune Responses and Inflammatory Diseases

The anthraquinones (AQs) and derivatives are widely distributed in nature, including plants, fungi, and insects, with effects of anti-inflammation and anti-oxidation, antibacterial and antiviral, anti-osteoporosis, anti-tumor, etc. Inflammation, including acute and chronic, is a comprehensive response to foreign pathogens under a variety of physiological and pathological processes. AQs could attenuate symptoms and tissue damages through anti-inflammatory or immuno-modulatory effects. The review aims to provide a scientific summary of AQs on immune responses under different pathological conditions, such as digestive diseases, respiratory diseases, central nervous system diseases, etc. It is hoped that the present paper will provide ideas for future studies of the immuno-regulatory effect of AQs and the therapeutic potential for drug development and clinical use of AQs and derivatives.     

Isolation and identification of anthraquinones of Caloplaca cerina and Cassia tora

Five hydroxyanthraquinone derivatives physcion, emodin, fallacinal, teloschistin, and 1,3-dimethoxy-8-hydroxy-6-methyl-9,10-anthraquinone were isolated from the lichen Caloplaca cerina growing in Serbia. Three anthraquinone derivatives, namely physcion, emodin, and rhein were isolated and identified from the Thai medicinal plant Cassia tora. Presented at the 33rd International Conference of the Slovak Society of Chemical Engineering, Tatranské Matliare, 22–26 May 2006.     

应用固定化Baker's酵母还原醌类化合物的研究

苯醌、萘醌和蒽醌类化合物在水溶液中能被固定化Baker's酵母还原。本文研究了影响固定化Baker's酵母还原活性、醌类化合物还原速度的因素及还原产物的分离和稳定性。