圆弧青霉突变株HB01碱性脂肪酶基因的克隆和表达

圆弧青霉(Peniciuium cyclopium)突变株可产生一种具有工业价值的碱性脂肪酶(alkaline lipase,PEL),通过RT-PCR基因克隆及序列测定,获得了PEL完整的基因序列,该基因全长855bp,编码1个由285个氨基酸残基组成的酶蛋白,根据氨基酸组成推导该脂肪酶蛋白的分子量约为30kD.     

When VSEPR Fails: Experimental and Theoretical Investigations of the Behavior of Alkaline‐Earth‐Metal Acetylides

The synthesis, structural, and spectral characterization as well as a theoretical study of a family of alkaline‐earth‐metal acetylides provides insights into synthetic access and the structural and bonding characteristics of this group of highly reactive compounds. Based on our earlier communication that reported unusual geometry for a family of triphenylsilyl‐substituted alkaline‐earth‐metal acetylides, we herein present our studies on an expanded family of target derivatives, providing experimental and theoretical data to offer new insights into the intensively debated theme of structural chemistry in heavy alkaline‐earth‐metal chemistry.     

Charge‐Separated and Molecular Heterobimetallic Rare Earth–Rare Earth and Alkaline Earth–Rare Earth Aryloxo Complexes Featuring Intramolecular Metal–π‐arene Interactions

A new family in town! Treatment of a rare‐earth metal (Ln) and either a potential divalent rare‐earth metal (Ln′) or an alkaline earth metal (Ae) with 2,6‐diphenylphenol (HOdpp) at elevated temperatures (200–250 °C) afforded heterobimetallic aryloxo complexes (see figure). Both a charge‐separated species, [(Ln′/Ae)₂(Odpp)₃][Ln(Odpp)₄], and a neutral species, [AeEu(Odpp)₄], were obtained and crystallographically characterised.

     

新型铜配合物[Cu(PyPt)(NO3)2]合成、结构、表征及其与DNA相互作用

本文报道了新型铜配合物[Cu(PyPt)(NO3)2](HPyPt是N-2-吡啶基-N'-苯基硫脲)的合成、结构、波谱表征及其与DNA相互作用研究.该配合物属三斜品系P1空间群,其中a=0.761 52(15)nm,b=0.80957(16)nm,c=1.311 3(3)nm,α=76.01(3)°,β=89.59(3)°,γ=68.24(3)°,V=0.7255(2)nm3,Z=2.利用荧光光谱法、紫外光谱法和粘度法初步研究了[Cu(PyPt)(NO3)2]与小牛胸腺DNA的相互作用,计算其与DNA结合常数Kb为1.63×103 L·mol-1.     

Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Mercury Based on the Reaction between Alkaline Phosphatase and Mercury Using Triton X-100 as a Sensitizer

A new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace mercury has been established, using Triton X‐100 as a sensitizer. The regression equation of working curve was ΔI_p=11.40m(Hg²⁺)+1.569 (ag·spot^?1, n=6, ΔI_p=I_p1?I_p2, I_p1 and I_p2 referred to the phosphorescence intensity of the blank reagent and the test solution, respectively), and correlation coefficient (r) was 0.9984. The RSD valus of the determination of 0.016 and 8.0 ag·spot^?1 Hg²⁺ were 4.1% and 1.7% (n=8), respectively, indicating that the method had good repeatability. The limit of detection (LOD) calculated by 3Sb/k was 7.0 zg·spot^?1 Hg²⁺ (corresponding concentration: 1.8×10^?17 g·mL^?1, Sb=0.025, n=11). This method has high sensitivity, selectivity and precision, which was applied to determination of trace mercury in water samples with the result being agreed very well with that of dithizone extraction spectrophotometry.     

Synthesis and Antiviral Activities of Chiral Thiourea Derivatives

An environmentally benign method has been developed for the synthesis of novel chiral thiourea derivatives in high yields in ionic liquid [Bmim]PF6. The ionic solvent can be recovered and reused without any loss of its activity. The target compounds were characterized by elemental analysis, IR, 1H NMR and 13C NMR spectral data. According to the preliminary bioassay, some of the chiral thiourea analogues exhibited moderate in vivo antiviral activities against TMV at a concentration of 500 mg/L. Title chiral compound 3i was found to possess good in vivo protection, inactivation and curative activities of 57.0%, 96.4% and 55.0%, respectively against TMV with an inhibitory concentration at 500 mg/L. The title chiral compound 3i revealed better inactivation effect on TMV (EC50＝50.8 µg/mL) than Ningnanmycin (EC50＝60.2 µg/mL).     

The investigation on the relative stability of different clusters of thiourea dioxide in water using gas phase quantum chemical calculations

The relative stability of different clusters of thiourea dioxide (TDO) in water is examined using gas phase quantum chemical calculations at the MP2 and B3LYP level with 6‐311++G(d,p) basis set. The possible equilibrium structures and other energetic and geometrical data of the thiourea dioxide clusters, TDO‐(H₂O)_n (n is the number of water molecules), are obtained. The calculation results show that a strong interaction exists between thiourea dioxide and water molecules, as indicated by the binding energies of the TDO clusters progressively increased by adding water molecules. PCM model is used to investigate solvent effect of TDO. We obtained a negative hydration energy of ?20.6 kcal mol^?1 and free‐energy change of ?21.0 kcal mol^?1 in hydration process. On the basis of increasing binding energies with adding water molecules and a negative hydration energy by PCM calculation, we conclude thiourea dioxide can dissolve in water molecules. Furthermore, the increases of the C? S bond distance by the addition of water molecules show that the strength of the C? S bonds is attenuated. We find that when the number of water molecules was up to 5, the C? S bonds of the clusters, TDO‐(H₂O)₅ and TDO‐(H₂O)₆ were ruptured. © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2009     

Synthesis of thiourea derivatives of the alkaloid anabasine and crystal structure of <Emphasis Type="Italic">N</Emphasis>-(anabasino1-thiocarbonyl)furan-2-carboxamide

A series of new N-acylsubstituted thioureas, the compositions and structures of which were determined by IR and PMR spectroscopy and mass spectrometry, were synthesized from the alkaloid anabasine. The crystal structure of one of the products, N-(anabasino-1-thiocarbonyl)furan-2-carboxamide, was confirmed by x-ray structure analysis. It was also shown that this compound exhibited moderate antibacterial activity. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 183–185, March–April, 2009.     

杂环苄基锡二氨基硫脲席夫碱配合物的合成

二氨基硫脲与芳香醛(酮)反应,合成了3个三齿Schiff碱配体;配体与二苄基二氯化锡或三苄基氯化锡反应合成了6个未见文献报道的三齿配体杂环有机锡(Ⅳ)配合物,其结构经1H NMR, IR和元素分析表征.     

添加剂丙烯基硫脲对镍电沉积的影响研究

采用循环伏安、线性扫描和恒电位阶跃电化学方法结合扫描电镜研究了不同浓度的丙烯基硫脲(ATU)对NH₃-NH₄Cl-H₂O体系镍在玻碳电极上的电沉积过程的影响. 循环伏安测试、线性扫描以及恒电位暂态曲线一致表明ATU的加入对镍电沉积具有阻化作用,并且随着ATU浓度的增加其阻化作用增强;恒电位暂态曲线结果表明,镍的电结晶是按瞬时形核三维生长机理进行的,随外加电位负移,晶体向外生长速率增大;ATU的加入没有改变镍的形核方式,但形核数密度增大,并且减小晶体向外生长的速率;扫描电镜结果表明,ATU的加入可以细化晶粒,得到整平、致密的镍沉积层.